Lecture notes and recordings
Lecture notes and recordings PCB 3063
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This 6 page Bundle was uploaded by Jisun Ban on Saturday October 10, 2015. The Bundle belongs to PCB 3063 at University of North Florida taught by Waddell in Fall 2015. Since its upload, it has received 218 views. For similar materials see Genetics in Biology at University of North Florida.
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Date Created: 10/10/15
1 True or False A typical chromosome can contain several hundred to several thousand genes arranged in linear order along the DNA molecule present in the chromosome True 2 True or False The sequence of nucleotides in the human genome is 909 identical from one person to the next False it s 999 identical 3 True or False Renaturation is the separation of DNA strands False 4 True or false DNA polymorphisms are rare in natural populations of most organisms False 5 True of false Most genes are present in pairs in the nonreproductive cells of most animals and higher plants True 6 LD rnonwzp po rnonwgt 5 rI39IUOwZD rnonwgt Which of the following is false regarding the denaturation of DNA DNA can be denatured with high heat DNA can be denatured by high pH denaturation of DNA can be detected by measuring the UV absorbency at 260 nm denaturation disrupts the hydrogen bonds holding the strands together DNA can be denatured by low salt conditions V Which of the following is false regarding Tm The Tm represents the midpoint of the narrow temperature range at which DNA denatures The Tm increases with G C content The Tm decreases with G C content V The Tm decreases with the A T content Tm can be determined by monitoring the change in UV absorbency at 260 nm DNA fragments migrate in an electric field because DNA molecules are negatively charged V are positively charged are neutral in charge are double stranded contain numerous covalent bonds Endonucleases remove amino acids from the carboxyl end of a polypeptide chain connect two DNA fragments together remove terminal nucleotides from the 339 end of a polynucleotide chain connect two oligopeptides together break internal phosphodiester bonds in a single or double stranded nucleic acid molecule V 10 Which of the following statements about PCR is false Page 1 PCR stands for polymerase chain reaction PCR uses short synthetic oligonucleotide primers PCR uses a DNA polymerase to synthesize DNA PCR can be used to obtain large quantities of a particular DNA sequence PCR does not require knowledge of the DNA sequences at the ends of the region to be amplified V WUPP 12 Which chemical group is at the 539 end of a single polynucleotide strand hydroxyl group phosphate group V diester group purine base nitrogen group Wong 13 Which chemical group is at the 339 end of a single polynucleotide strand phosphate group hydroxyl group V purine base nitrogen group methyl group WUDPUP 14 The purine bases are thymine and cytosine thymine and guanine adenine and guanine V cytosine and adenine cytosine and guanine rnonwgt 15 The pyrimidine bases are thymine and cytosine V thymine and guanine adenine and guanine cytosine and adenine cytosine and guanine rnonwgt 16 What is the base sequence of a DNA strand that is complementary to 539 ATCGGATTC 339 sequence A 539 ATCGGATTC 339 B 539 CTTAGGCTA 339 C 539 GAATCCGAT 339 V D 539 TAGCCTAAG 339 17 If guanine makes up 23 of the nucleotides in a sample of DNA then thymine would make up what percentage of the bases Page 2 A 22 B 30 c 40 D 27 V E 15 18 Which of the following is not true regarding the structure of DNA A Each chain makes one complete turn every 34 angstroms B Two strands are antiparallel C onic bonds are the main force holding the strands together V D The purine adenine base pairs with the pyrimidine thymine E The paired bases are parallel to one another and perpendicular to the long axis of the helix 19 A palindrome is a sequence that reads differently when read from either direction the type of sequence recognized by most restriction enzymes a sequence that is the same when read from either direction a sequence that is used for DNA synthesis both B and C V W U n w gt 20 Which of the following sequences are palindromes A 539 CCAG 339 B 539 CCCC 339 C 539 CCAGG 339 D 539 CCATGG 339 V E 539 ACCGGA 339 21 What kind of ends are produced by the restriction enzyme Cfol which recognizes the sequence 539GCGJC 339 and cleaves each strand of DNA helix at the site represented by the downward arrow A Blunt ends B 539 overhang C 339 overhang V D none ofthe above 22 Which of the following is a characteristic of restriction enzymes A most restriction enzymes recognize a single site B the restriction site is recognized regardless of the source of the DNA C the number of times a piece of DNA is cut is determined by the number of times the restriction site is present in the DNA D most restriction sites are palindromes E A B C and D V F A and C G B and D Page 3 23 539 ACG 339 is half of a palindromic restriction site What is the complete sequence A 539 ACGACG 339 B 539 ACGCGT 339 C 539 ACGGCA 339 D 539 ACGTGC 339 24 In a random sequence consisting of equal proportions of all four nucleotides what is the average distance between consecutive occurrences of the restriction sites for the Hinfl 539 GlANTC 339 where N stands for any nucleotide and the downward arrow represents the cleavage site A 64 bp B 128 bp C 256 bp D 1024 bp 25 In a random sequence consisting of equal proportions of all four nucleotides what is the probability that a particular short sequence of nucleotides matches a restriction site for a restriction enzyme with a 4 base cleavage site A 04 B 025 c 05 D 044 E 0254 F 054 28 The physical or biochemical expression of the genotype is called the A phenotype B homozygous allele C heterozygous allele D transcription E single nucleotide polymorphisms 29 A is defined as any difference in DNA no matter how it is detected whose pattern of transmission from generation to generation can be tracked A mutation B genetic marker C genetic drift D consensus sequence 30 The detection of DNA markers usually requires that be fragmented into pieces of manageable size that can be manipulated in laboratory experiments Page 4 A ribosomes B mRNA C genomic DNA D mitochondrial DNA 31 The presence of a primer with a free 339OH group is essential for DNA polymerase to synthesize DNA since no known DNA polymerase is able to initiate chains 32 A restriction enzyme is a nuclease that recognizes a short nucleotide sequence in a DNA molecule and cleaves the molecule at that site 33 The technique used to separate molecules on the basis of their different rates of movement in an electric field is known as electrophoresis 34 Synthesis of DNA is catalyzed by an enzyme called and synthesis of RNA is catalyzed by an enzyme known as RNA polymerase 35 The thymine found in DNA is replaced in RNA by the closely related pyrimidine uracil 36 According to Chargaff39s rules the amount of guanine in doublestranded DNA molecule equals that of cytosine and the amount of thymine is the same as that adenine 37 DNA strands are said to be antiparallel because the phosphate linkages in the backbones run in opposite directions 39 The alternative forms of a gene are called alleles of the gene 40 The initial approach to the identification of a disease gene is to find DNA markers that are genetically linked with the disease gene in order to determine its chromosomal location a procedure known as mapping 41 Restriction enzymes that have symmetrical cleavage sites yield DNA fragments that have blunt ends 42 The method for selective amplification of a DNA fragment is called the polymerase chain reaction 43 A Single Nucleotide Polymorphism or SNP is present at a particular nucleotide site if the DNA molecules in the population often differ in the identity of the nucleotide pair that occupies the site 44 Because of their high degree of variation among people STRs are widely used in DNA fingerprinting to establish an individual39s identity for use in criminal investigation and parentage determinations 45 The utility of DNA markers in locating and identifying disease genes results from genetic linkage which means the tendency for genes that are close together in a chromosome to be inherited together Page 5
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