Notes for class (10-13)
Notes for class (10-13) BIOL 4801 A
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This 2 page Bundle was uploaded by Katherine on Tuesday October 13, 2015. The Bundle belongs to BIOL 4801 A at Indiana University of Pennsylvania taught by Dr. Ruby in Fall 2015. Since its upload, it has received 25 views. For similar materials see Special Topics in Biology at Indiana University of Pennsylvania.
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Date Created: 10/13/15
Habenular a5 nicotinic receptor subunit signaling controls nicotine intake Withdrawal symptoms include anxiety depression trouble sleeping headaches increased appetite and problems concentrating A5 nAChRs neuronal nicotinic acetylcholine receptors Ligand gated ion channels encode by CHRNA5 A513B4 nAChR subunit variations increase the risk of tobacco addiction Studied quotmore extensively that other members of the nicotinicoid family of receptors 1 Habenula a Posterior to the thalmus b Associated with the pineal gland c Role in quotdecision making by regulating dopaminergic These two together are activated by high doses of nicotine Animals a Male and female mice i A5 knockouts and wildtypers ii Male Wistar rats 4 Self administration a Self administration of nicotine infusions b Autoshaping 1 hr daily sessions 5 Lentivirus administration a Lentivirus are effective gene delivering vectors b For quotrescuequot experiment CHRNA5 was cloned in to the lentivirus c For quotknockdownquot studies shRNAs were put against CHRNA5 d Injections of lentivirus in the MHb 6 Radiolabelling a 86Rb to measure nAChR signaling b Synaptosomes generated from lPN MHb hippocampus striatum thalamus and cortex 7 BSR electrodes a Electrodes implanted in the intracranial cavity b Tested reward thresholds 8 Using self administration mice consumed about 15 mg 9 Knockout mice consumed greater amounts a Higher motivation to seek out drug b lntensi cation of reinforcing properties 10Knockouts a Less dramatic increase the nicotine intake keeps increasing for the wild types b Lentivirus infection cells were found with in the habenula interpeduncular region of the knockouts 11Control and nicotine intake a Function and expression can be rescued via the lentivirus 12A6 nAChRs inhibit brain reward function a Brain stimulation reward thresholds after knockdown b Low doses of nicotine lowers BSR thresholds i Similar with lentivirus rats c Higher doses of nicotine lowers BSR thresholds UJN d No way to stop yourself from overconsumption e Higher density of nicotine within the wild types High doses of drugs make animals stress and stop the knock outs don39t care as much 13ntroduced lidocaine to the IPN and MHb increased responding for nicotine a VTA inactivation decreased nicotine intake 14NMDA lead to increased nicotine at higher dose 15Concusions a The habenulointrpeducular pathway as a kaye neurocircuit controlling nicotine intake b Individuals de cient in a5 nAChRs are insensitive c Malefemale mice v just male rats
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