Lab Exam 1
Lab Exam 1 Microbiology 20214
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This 3 page Study Guide was uploaded by Jade Frederickson on Tuesday January 19, 2016. The Study Guide belongs to Microbiology 20214 at Texas Christian University taught by Dr. Curtis in Fall 2015. Since its upload, it has received 59 views. For similar materials see Intro to Microbiology in Biology at Texas Christian University.
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Date Created: 01/19/16
Lab Exam # 1 Question and Answer Guide Exercise 2. Use of Compound Microscope 1. Name the four types of lenses and their magnification, and the total magnification of each lens. 2. Label and describe: eyepiece (10X), fine focus, coarse focus, objective lenses, stage, adjustments for mechanical stage, condenser, iris diaphragm for condenser, and rheostat 3. What is the refractive index? Why is oil needed for the 100X lens? Exercise 3. Bacteria and Bacterial Structures (prepared slides) 1. Explain the depth of focus and why it is important in viewing the strings. What is the order of the strings? 2. Name the three morphologies of bacteria. Identify strept- and staph- 3. Name the structures of endospores, flagella, and the cell capsule. What should you be looking for in each stain? 4. Name the two types of fungi and protozoa examined. Exercise 4. Sampling the Environment 1. Microorganisms are ______________ meaning that they: 2. How should plate samples be stored? What are the incubation procedures? 3. How can colony morphology be described? (at least three different ways) Exercise 5. Aseptic Technique 1. What does asepsis mean and why do scientists do it? Why does proper aseptic technique yield? 2. How do you sterilize the transfer loop? 3. How else do you increase chances of avoiding contamination? 4. Where are microorganisms usually transferred from? 5. If a culture is on a slant or a plate, growth will be ____________________________. This means that you should not ____________________________________________________________. 6. What is better: a large gob or a small loopful? Why? Exercise 6. Pure Culture Methods (Streak Plates) 1.
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