Study Guide for BIOC 462A at UA 2
Study Guide for BIOC 462A at UA 2
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Date Created: 02/06/15
ENZYMES SELF STUDY QUESTIONS 1 A chemical reaction has a A G0 2000 ldmol If this were an enzymecatalyzed reaction what can you predict about the kinetics a It will exhibit very rapid kinetics b It will exhibit very slow kinetics c The kinetics of the reaction can not be predicted d The kinetics depend on the nature of the reactants e The kinetics depend on the nature of the products 2 Ifthe standard free energy for an uncatalyzed reaction is 2500 kJmol then what can you say about the standard free energy for the enzymecatalyzed reaction 3 Ifthe activation energy for an uncatalyzed reaction is 25 ldmol then what can you say about the activation energy for the enzyme catalyzed reaction 4 Is it possible for pH to affect the activity of an enzyme in a range where none of the side chain groups of the protein are titrated Explain 5 Explain the following observation A rule of thumb is that a chemical reaction will go twice as fast for every 100 rise in temperature This is true for enzymes up to a certain temperature at which point the reaction no longer occurs 05 Assuming they have equal affinity for the enzyme why would a noncompetitive inhibitor be a more effective drug than a competitive inhibitor 7 Why is it advantageous to the cell to produce proteolytic enzymes in the form of zymogens 509 Why does Vm but not Km depend on the amount of enzyme used in an enzyme reaction Km and Vm for two substrates A and B are 40 M 25 u molsec and 05 mM 15 u molsec respectively At low concentration lt1 mM which substrate will react most rapidly 10 TosylLphenylalanine chloromethyl ketone is a specific inhibitor of chymotrypsin a Why is this compound specific for chymotrypsin b Can you suggest a way to make a similar compound that would be specific for trypsin 11 The following table presents the rates v u molsec at which an enzyme converts its substrate to product Rxn 1 represents data for the uninhibited reaction and Rxns 2 and 3 represent data collected in the presence of two different inhibitors each present at lOmM Assuming the amount of enzyme is the same in each case determine Km and Vm for the enzyme and for each inhibitor determine K and the type of inhibition S mM Vrxn 1 V rxn2 V rxn3 1 H 25 2 H 40 5 63 10 H 76 20 H 90 l2 Methanol is oxidized by alcohol dehydrogenase ADH to the highly toxic formaldehyde Drinking methanol is fatal because of the production of formaldehyde methanol itself is hannlzss and 5 excntzdbythz whey ADII wd alsa axmud athzx alcahnls sh 5 eemx a Based an he mfannman pmpuse 5 mm mm an enema whn has Weed meer b What addmaml mfannmmn vmuld yml medbefm sh a hemmem cmddbe mam mswms 1 m n 12 e m can mt mkz mypxedwonns sham kmzucs mm thznnndymmm am slam because A 339 dues mt dzpnd an m pane mm reactams m mam whzxeas u mmsdmsdzpndanthz Fem n w be me am Enzymzs amyaccelzmz me we at whmh a alumna reasonn reachas eqmllhn lm mt u eqmllhnnm pusman n mustbe 1mm Enzymes accelzmm nutmn nus bylawznng me emm snugny me reasonn Yes1 hz subsmhe meme mmeem farm maxdznabmdm me enzymz Enzymes are pmtems andm hlghtzmpnmre me enzymz ls damth Th2 presence af meme wmdd mum effechvemssa hz mmmpeme mm Th2 presence a hz meme wmdd mt 51M me effechvenzss a hz mmmpemwe mam Because me zymngens m Inactive Mpmhects me eeu mm pmtzalync dzgndmmn Vcamams a m mm m cancemnnan afenzymz v mag K 15 a meme cancemnonn and 139s dz nmnn dnesnm malqu Mennfanhz camenmtmnafenzyme abeeme VmKmxs 3n faxsubslmz Emit 25 fars nsmm A Thzm39axe at law ememm s nsmte a wA be used mm ef clzndy a This eamwmbm m u spcx cnypuckzt afchymnh39ypsmvm ag Flag gmnp b Rzplace me Flu wnh an Axg whwh wmddbmd m txypsm s spcx cnypuckzt K 32mm quotquot m maysee m taxman 2 me mum ls eampenme wnha K1 7 5 mm m taxman 3 me mum ls mmmpeme wnh K 5 mM man u Rllclhnl n new are m m u ms u u a 1m mam were gwene uml u ethamlvmuldacns acamptmve mum armeumx madman mm m hdnzys a exexece u mum b m wuld med m kmw me mauve K afADH fax alumni and mm m mde m kmw hnwmvch ethamha gm sa as m prevemmzthaml madman
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