Study Guide for BIOL 420 at KU
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This 1 page Study Guide was uploaded by an elite notetaker on Friday February 6, 2015. The Study Guide belongs to a course at Kansas taught by a professor in Fall. Since its upload, it has received 14 views.
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Date Created: 02/06/15
Biology 420 Biochemistry Seminar Spring 2003 Dr G 80l2 Haw 864 3939 pgegenkuedu Study guide for Piccirilli I993 Piccirilli et al I993 Premise What is the basic biochemical question these researchers are trying to answer a What are the two main roles of divalent metal ions in RNA catalyzed reactions b Why is it difficult to differentiate between these roles Background What led the authors to perform this study a What is the Tetrahymena self splicing intron b How is this particular construct made into a two component enzyme plus substrate Technical What methods were used to answer the questions being asked a How does the sulfur substitution used in this experiment differ from that used by Harris b How were the first order chemical rate constants determined c How was enzymesubstrate binding measured Results What key experiments did the researchers do to answer their question a What is the key experiment that the authors do to answer this question b What are the two key points of evidence that a metal ion makes a direct inner sphere coordinate bond with the 5 oxygen c What is the thio effect How was the role of metal ion determined to be in catalysis rather than in enzymesubstrate binding Outcome How did the data obtained answer the original question a What roles do the authors propose for Mgll ion b What evidence is there for involvement of more than one metal ion in the reaction c Do the data actually demonstrate that the intron is a true metalloenzyme
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