New User Special Price Expires in

Let's log you in.

Sign in with Facebook


Don't have a StudySoup account? Create one here!


Create a StudySoup account

Be part of our community, it's free to join!

Sign up with Facebook


Create your account
By creating an account you agree to StudySoup's terms and conditions and privacy policy

Already have a StudySoup account? Login here

BIOL 2281 Midterm 1

by: Ming-Han Lu

BIOL 2281 Midterm 1 BIOL 2281

Ming-Han Lu
GPA 3.96

Preview These Notes for FREE

Get a free preview of these Notes, just enter your email below.

Unlock Preview
Unlock Preview

Preview these materials now for free

Why put in your email? Get access to more of this material and other relevant free materials for your school

View Preview

About this Document

Hey guys! I finished this study guide pretty quickly, and I incorporated some of my BIO 1 notes into it. It has some diagrams and pictures in it, so if you have any questions, feel free to send me ...
Introductory Biology Laboratory
Wen Lin, Elizabeth Pickett
Study Guide
Biology, sciences
50 ?




Popular in Introductory Biology Laboratory

Popular in Biology

This 6 page Study Guide was uploaded by Ming-Han Lu on Monday October 10, 2016. The Study Guide belongs to BIOL 2281 at University of Texas at Dallas taught by Wen Lin, Elizabeth Pickett in Fall 2016. Since its upload, it has received 143 views. For similar materials see Introductory Biology Laboratory in Biology at University of Texas at Dallas.


Reviews for BIOL 2281 Midterm 1


Report this Material


What is Karma?


Karma is the currency of StudySoup.

You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!

Date Created: 10/10/16
Introductory Biology Laboratory BIOLOGY 2281 Fall 2016 Class Information Lecture:  Monday: 12:00­12:50 P.M. at SLC 1.102 Lab:  at SLC 2.215       See Table below:  Day of the Section # Time Instructor Week Monday 102 1:00 PM – 3:45 PM  Dr. Lin Monday 109 4:00 PM – 6:45 PM  Dr. Hill  Tuesday 103 10:00 AM –12:45 PM Dr. Hill Tuesday 104 1:00 PM – 3:45 PM Dr. Hill Tuesday 101 4:00 PM – 6:45 PM Dr. Lin Wednesday 105 10:00 AM –12:45 PM Dr. Lin Wednesday 106 1:00 PM – 3:45 PM Dr. Lin Wednesday 110 4:00 PM – 6:45 PM Dr. Pickett  Thursday 107 10:00 AM –12:45 PM Dr.Lin Thursday 108 1:00 PM – 3:45 PM Dr.Lin Friday 111 9:00 AM – 11:45 AM Dr. Pickett Instructor Contact Information  Dr. Wenju Lin  Dr. Elizabeth Pickett  Dr. Brenna Hill Instructor Office hours and locations: (announced on Aug 29)  THE FIRST MONDAY MEETING STARTS ON AUG 29 IN SLC 1.102  THE FIRST LAB STARTS ON  Aug 29, 30, 31 and Sep 1, 2 IN SLC 2.215.  There are no labs on the week of Sep 5, 6, 7, 8, 9. Your Section’s Teaching Assistants Name Email Address COURSE PRE­REQUISITES: BIOL 2311 COURSE DESCRIPTION The primary goal of this semester­long course is to provide you with opportunities to learn bioinformatics and various laboratory skills and techniques used in molecular biology.  Lectures discuss the theoretical  1 aspects of the experiments carried out in the laboratory. Each laboratory experience builds or  interconnects with the others and seeks a balance between biological content and conceptual  understanding. The curriculum is tailored to the mission and strengths of the Department of Molecular  and Cell Biology at the University of Texas at Dallas.   STUDENT LEARNING OBJECTIVES/OUTCOMES Objectives: The goal of this course is to give students opportunities for hands­on learning of  biological principles. This course teaches students the basic concepts of bioinformatics; the  microbiological concepts and techniques such as microscopy and aseptic handling of  microorganisms; bacterial transformation; eukaryotic cell divisions; biochemical concepts and  techniques such as properties and identification of macromolecules, determination of the rate of an  enzyme­catalyzed reaction and protein gel electrophoresis; DNA­centered molecular biology  principles and techniques including polymerase chain reaction, restriction digestion, plasmid mapping  and DNA agarose gel electrophoresis. Each laboratory experience builds or interconnects with the  others and seeks a balance between biological content and conceptual understanding.    Outcomes: Students will therefore: 1. Be able to define, explain, and give examples of the basic concepts in bioinformatics, structure and properties of biologically important macromolecules, enzyme kinetics, eukaryotic cell divisions and  bacterial transformation, and polymerase chain reaction. 2. Be able to perform basic molecular biology techniques in DNA manipulation. 3. Be able to use common biological laboratory skills, techniques and instrumentations. 4. Learn how to properly present and process data, interpret data analytically and draw appropriate  conclusions.   COURSE MATERIAL  Biology 2281 Lab Manual, 2016 by Dr. Wenju Lin, Dr. Elizabeth Pickett, Dr. Brenna Hill and Dr.  Alice Zhou: Files of lab procedures and lecture slides will be posted at on  Aug 20, no purchase required. Printed Lab procedures are required for each lab exercise.  Several topics include pre­lab, graph paper and report pages that need to be printed on letter or A4 size paper single­sided .  Adobe reader needed.   Starting Fall 2016, NEW Classroom Polling Software Turning Point Cloud requires students  respond with Turning Technologies ResponseCard RF LCD (“clicker”) OR with a mobile  device. Both options will be supported in BIOL2281. Students must create an account with  the system through eLearning. Every student must have a Turning License in order to  activate their account. Students are expected to have a license (and clicker OR smartphone  app) by lecture #2 and to use it in every lecture session for the duration of the semester.   Below is the UTD Bookstore’s list of options for students and the price:   1. Clicker device + 1 year Turning License - $49.75 2. Clicker device + 2 year Turning License - $62.00 3. Used rental Clicker device - $19.60 4. 1 year Turning License - $39.25 5. 2 year Turning License - $43.00 Options 4 & 5 would be applicable if decide to use ONLY their mobile devices to respond.  2 The Bookstore will provide a limited number of free licenses on a first­come, first­served  basis to students who have previously purchased a clicker device from them.  Suggested reference books: Textbook for BIOL2311 or Biology, Raven et al., 8  Edition, McGraw­Hill, 2007. ISBN: 0­07­ 291845­4 COURSE EVALUATION/GRADING SCHEMES Students may earn a maximum of 505 points.  The following table lists the details of assessment items and  the point distributions.  The final grades for the course will be assigned as follows: (note partial grades such  as A­ or B+ etc will be issued and each letter grade listed below includes partial grades) A (A­, A, A+):  450­505;  B (B­, B, B+): 400­449;  C (C­, C, C+): 350­399; D (D­, D, D+): 300­349; F : 0­299 Assessment Activity Points Your points Mid­term Exam (including lab practicals) 100 Final Exam (including lab practicals) 110 10 out of 11 Post­Lab Reports (@ 20 pts each) 200 9 out of 10 Pre­lab/ quizzes (@ 10 pts each) 90 Clicker points 5 Total 505  Post­Lab Reports:  The format of lab reports will vary from week to week depending upon the type of  experiment that was performed. Although lab exercises will be done in groups of two or more students,  each student must turn in his or her own typed report for grading. Your report should reflect your  independent processing and presentation of data and answering related questions posted for current  semester. Do not copy material from other students. Do not allow any other student to see or copy your  work.  Any form of scholastic dishonesty will not be accepted.  Your work will be graded based on  neatness, accuracy and completeness. If you are not physically present during a particular lab, you are not entitled to turn in a post­lab report for credit.  One of the lowest report grades except report  E11 will be dropped.   Lab Exams:  Midterm and final exams will be given during the scheduled lab periods. Each exam will be composed of two parts: a lab practical part and a written test. The lab practical portion will carry  approximately 1/3 of the total exam grade and will focus on important laboratory techniques. The format  of the written test will be primarily short answers and may include diagrams and illustrations.  They are  designed to evaluate your understanding of the basic biological concepts and laboratory methodologies. You are responsible to study the contents of the lab sessions that you fail to attend.  Pre­Lab Assignments/Quizzes:  Most of the lab exercises will have either pre­lab assignments or pre­ lab quizzes to make sure that you are prepared for the experiment before you come to the lab.  Pre­labs and quizzes are INDEPENDENT work.  Do not copy material from other students. Do not allow other  students to copy your work. You must turn in the completed “Pre­lab” at the beginning of the scheduled  lab. Quizzes will also be administered at the beginning of lab. Students arriving late will not be given  extra time to complete the quiz. One of the lowest pre­lab/quiz grades will be dropped. 3  eLEARNING: All course related lecture notes and other material including announcements, photos of  the lab results, review questions, and all of the grades will be promptly posted on eLEARNING. Please  check it regularly. Contact your instructor with concerns regarding grades as soon as possible. Grades  regarding E1­E5 will be finalized on Oct 21 , grades regarding E6­E11 will be finalized on Dec 2.   COURSE POLICIES  Attendance and Class Participation: Attendance of all lecture and laboratory sessions ON TIME is  extremely important and thus mandatory, and will be recorded for each lab period. Your performance in  the course is dependent on your attendance, so please make every effort to attend all classes as  scheduled. Moreover, you are also expected to actively participate in all class activities.  If you miss the first meeting of the lab due to late registration, it is your responsibility to contact your  instructor by e­mail BEFORE Sep 5.  Failure to do so will result in lost points in the course and will  negatively impact your grade.   Switching lab sessions after your scheduled lab is over is strictly prohibited.  Switching is only allowed if  you have a valid reason such as a medical/graduate school interview or a planned medical treatment.   To get approval for any section switch, you must inform your instructor at least three days before your  planned absence. You will have the opportunity to earn points based on your participation in the lecture class as  documented via use of Turning Point. You will not be able to earn participation points if you fail to bring  your personally registered device to lecture. Additionally, you will not earn participation points if you fail  to properly utilize your device during lecture (i.e., turning it on, electronically registering in class,  responding to questions, etc.).  Each question is worth 1 point – 0.5 points for responding and 0.5 points for correct answers. Students who earn between 70%­100%, 60­69%, 50­59%, or 0­49% of the total  possible points will receive 5, 3, 1, or 0 course points respectively.  Points will be awarded during  lectures #2­11.  Practice questions designed to accustom you to using the Turning Point response  system will be presented during lecture #1, but will not contribute to your score.  Pre­read:  Before you come to each lab, read the procedural handout for background information and  procedures for the experiment you will be doing.  This helps you not only do well on the pre­lab quizzes  or pre­lab assignments, but also to save you time and avoid unnecessary mistakes during the lab.  Part of the lab procedures can include assignments that are due at the beginning of the lab session  or the report pages that will be completed in the lab session.   Late work:  Pre­labs are due at the beginning of your lab session.  No late pre­labs will be accepted.   Post­lab reports are due at the beginning of the next scheduled lab unless otherwise noted (Report 4  and Report 11 are due at the end of lab session). Make a complete copy of your lab report before  turning it into the TA. Any post­lab reports that are late will be assessed a 3­point penalty for each DAY  they are late. Arrange the time and location with your graduate TA to turn in your late reports.  It is your  responsibility to confirm that your TA actually received your late reports.   Missed Exam/Quiz No make­up exams or quizzes will be administered.   Lab Safety: See handout provided on eLearning. Safety glasses will be provided in the laboratory. 4 COURSE OUTLINE/CALENDAR  THE FIRST MONDAY MEETING STARTS ON AUG 29 IN SLC 1.102  THE FIRST LAB STARTS ON  Aug 29, 30, 31 and Sep 1, 2 IN SLC 2.215.  There are no labs on the week of Sep 5, 6, 7, 8, 9. Week of  Monday through Friday Lab Exercises and Pre­Lab or Report Monday Exams Quiz  due (in Lecture Topic lab) Aug 29­Sep2 E1:  Bioinformatics Aug 29:  E1  Sep 5­9 No labs on Sep 5, 6,7, 8, 9 Sep 5:No lecture Sep 12­16 E2:  Microscopy Pre­Lab E2 R1 Sep 12: E2 Sep 19­23 E3: Microbial Techniques Quiz ­ E3 R2 Sep 19: E3  Sep 26­30 E4:  Eukaryotic Cell Divisions  Quiz ­ E4 R4 Sep 26: E4  Analysis of E3 results Oct 3­ 7 E5:  Restriction Enzyme Digest and Plasmid  Pre­lab E5 R3 Oct 3: E5  Mapping Oct 10­ 14 Mid­term Exam (E1­E5) /  lab practical  R5 Oct 10: E6  Oct 17­21 E6:    Biochemical Testing of Macromolecules  Pre­lab E6 Oct 17: E7 E7:   Extracting and Amplifying mtDNA Day 1 Quiz ­ E7 Oct 24­28 E7:  Extracting and Amplifying mtDNA Day 2 Quiz ­ E8 R6 Oct 24: E8 E8:  Bacterial  Transformation Calculation sheet Oct 31­Nov 1­4 E9:  Spectrophotometry Pre­lab E9 R7 Oct 31: E9  Analysis of E8 results Nov 7­11 E10:   Enzyme Assay  Quiz ­ E10 R8, R9 Nov 7: E10  Nov 14­18 E11:   Protein Separation by Gel  Pre­lab R10, Nov14: E11 Electrophoresis E11 R11 Nov 21­25 No labs Nov 21: No lecture Nov 28­Dec 2 Final Lab Exam/Lab Practical (E5  Nov 28: No lecture Electrophoresis, E6­E11) Abbreviations: E= Experiment; R=Report  Report 4, Calculation sheet of E8 and Report 11 are due at the end of assigned lab hours 5 The information contained in the following link constitutes the University’s policies and procedures segment  of the course syllabus.  Please go to­policies for these policies. These descriptions and timelines are subject to change at the discretion of the Professors.  6


Buy Material

Are you sure you want to buy this material for

50 Karma

Buy Material

BOOM! Enjoy Your Free Notes!

We've added these Notes to your profile, click here to view them now.


You're already Subscribed!

Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'

Why people love StudySoup

Bentley McCaw University of Florida

"I was shooting for a perfect 4.0 GPA this semester. Having StudySoup as a study aid was critical to helping me achieve my goal...and I nailed it!"

Jennifer McGill UCSF Med School

"Selling my MCAT study guides and notes has been a great source of side revenue while I'm in school. Some months I'm making over $500! Plus, it makes me happy knowing that I'm helping future med students with their MCAT."

Steve Martinelli UC Los Angeles

"There's no way I would have passed my Organic Chemistry class this semester without the notes and study guides I got from StudySoup."

Parker Thompson 500 Startups

"It's a great way for students to improve their educational experience and it seemed like a product that everybody wants, so all the people participating are winning."

Become an Elite Notetaker and start selling your notes online!

Refund Policy


All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email


StudySoup has more than 1 million course-specific study resources to help students study smarter. If you’re having trouble finding what you’re looking for, our customer support team can help you find what you need! Feel free to contact them here:

Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to

Satisfaction Guarantee: If you’re not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.

Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.