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UF / Chemistry / CHM 3303 / in eukaryotic cells, where does the basal transcription apparatus bind

in eukaryotic cells, where does the basal transcription apparatus bind

in eukaryotic cells, where does the basal transcription apparatus bind

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AGR3303 Mid-term 2 09/23/2016 1. The function of DNA polymerase I involves removal of RNA primers (during DNA replication)  a. 5′ → 3′ exonuclease activity. b. 3′ → 5′ exonuclease activity. c. 5′ → 3′ telomerase activity. d. 3′ → 5′ telomerase activity. e. 5′ → 3′ gyrase activity. 2. For which of the following is the “end-replication problem” relevant? a. Circular DNA b. Linear chromosomes c. The centromere region of a chromosome d. Rolling circle model of replication e. Theta model of replication 3. Telomerase uses _____________ to synthesize new DNA. a. exonuclease activity b. a licensing factor c. strand invasion d. a DNA template e. an RNA template 4. DNA replication in eukaryotes differs from replication in prokaryotes in that: a. DNA replication in eukaryotes is conservative, whereas in prokaryotes it is semiconservative. b. Eukaryotes have bidirectional replication from an origin, whereas in prokaryotes replication always  proceeds in one direction from an origin. c. Eukaryotic chromosomes have many separate origins of replication, whereas prokaryotic chromosomes  have a single origin of replication. d. Linear eukaryotic chromosomes are replicated by a mechanism called theta replication, whereas circular  prokaryotic chromosomes are replicated by the rolling circle mechanism. 5. What would the result be if a specific sigma subunit of bacterial RNA polymerase were mutated? a. Nothing would result; the sigma subunit is not essential. b. RNA polymerase would still bind DNA at specific sites, but will fall off after joining together only a few  RNA nucleotides. c. RNA polymerase would initiate transcription at random locations on the DNA. d. The core enzyme would not be as stable as usual. 6. In bacteria, the elongation stage of transcription: a. Is carried out by the RNA polymerase core enzyme. b. Involves unwinding of only short regions of the DNA by RNA polymerase. c. Generates positive supercoils ahead of the transcription bubble.d. Formation of DNA-RNA hybrid in transcription bubble. e. All of the above. 7. A rho-independent transcription terminator includes: a. inverted repeats that fold into a hairpin when transcribed. b. a string of 7–9 U’s in the DNA template. c. a region in the RNA molecule that causes RNA polymerase to pause. d. All of the above. e. Both a and c. 8. In eukaryotes, transcriptional activator proteins do all of the following EXCEPT: a. form part of the RNA polymerase II holoenzyme. b. bind either the regulatory promoter or enhancer of a gene. c. increase the rate at which transcription is initiated by RNA polymerase II. d. b & c 9. While very similar in fundamental aspects, bacterial and eukaryotic gene transcription differ in that: a. Bacterial transcripts are always 3′ → 5′, whereas eukaryotic transcripts often 5′ → 3′. b. In eukaryotes, the chromosome is modified to expose DNA before transcription, whereas a prokaryotic  chromosome does not need modification. c. Only small regions of the bacterial chromosome are unwound at a time by RNA polymerase, whereas in  eukaryotes RNA polymerase unwinds large regions of the DNA at a time.  d. b & c 10. Where are promoters usually located? a. Upstream of the start site b. Downstream of the start site c. Near nucleotide -100 d. Near the hairpin loop e. Downstream of the terminator 11. In eukaryotic cells, where does the basal transcription apparatus bind? a. Core promoter b. Regulatory promoter c. Terminator d. Enhancer e. Ribozyme 12. Which of the following statements is FALSE regarding TFIID? a. It contains a TATA binding protein. b. It aids in initiation of transcription. c. It binds to the core promoter. d. It binds to the TATA box. e. It is a transcriptional activator protein.13. What is the function of general transcription factors? a. They are DNA sequences to which RNA polymerase binds. b. They direct nucleosome assembly. c. They bind to regulatory promoters to increase the rate of transcription. d. They bind to enhancers to allow minimal levels of transcription. e. They are a part of the basal transcription apparatus. 14. Unlike transcriptional termination in prokaryotes, termination in eukaryotes (by RNA polymerase II): A. depends on a specific sequence of RNA. B. depends on a protein, Rat. C. involves cleavage of the pre-mRNA. D. involves exonuclease activity. E. All of the above. 15. Which of the following is a sequence of DNA where transcription is initiated? a. Hairpin loop b. TBP c. Initiator d. Sigma factor e. Promoter 16. The list of events below describes eukaryotic pre-mRNA processing. Please select the choice that lists the  events in correct sequential order. 1. Recognition and binding the 3′ AAUAAA sequence by specific protein factors  2. Cleavage at the poly(A) site  3. Addition of the 5′ cap  4. Export to the cytoplasm  5. Addition of the poly(A) tail  a. 3, 1, 2, 5, 4 b. 2, 3, 4, 5, 1 c. 4, 2, 3, 1, 5 d. 1, 3, 5, 4, 1 e. 5, 4, 1, 3, 2 17. Processing of pre-mRNA to produce mature mRNA includes all of the following EXCEPT: a. addition of the 5’ 7-methyl-guanylate cap. b. splicing of exons, along with removal of introns. c. addition of the 3’ poly-A tail. d. transport to the cytoplasm for translation. e. Actually, processing includes all of these. 18. Splicing requires all of the following EXCEPT: a. specific sequences at the 5’ and 3’ ends of introns. b. proteins that recognize the splicing sequences. c. two cleavages, excising the introns. d. ligation of the exons.e. Actually, all of these are required for splicing. 19. The human gene encoding for calcitonin contains six exons and five introns and is located on chromosome 11.  The pre-mRNA transcript from this gene can generate either calcitonin or calcitonin gene related peptide  (CGRP) in a tissue-specific manner. Calcitonin produced from the thyroid gland is 32 amino acids long and functions to regulate the calcium while CGRP, which contains 37 amino acids, is produced by the brain cells  and involved in transmission of pain. Which of the following processes makes production of two functionally  and structurally different proteins from the same gene possible? a. Self-spicing introns b. Differential transcription c. Alternative replication d. 5′ capping and polyadenylation e. Alternative RNA splicing 20. Eukaryotic posttranscriptional modification of pre-mRNA helps in following processes except a. Increase of mRNA stability b. Facilitate ribosome binding c. Removal of non-coding introns d. Facilitate degradation of mRNA e. All of the above  s yawla ton era snietorp gnidnopserroc eht dna secneuqes AND. 1: TPECXE gniwollof eht fo lla fo esuaceb setoyrakue ni raeniloc. srucco gnicilps. B. snortni yb detpurretni era secneuqes gnidoc. C. nietorp rof edoc ton od ANR eht fo snoitces. 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What would the result be if a specific sigma subunit of bacterial RNA polymerase were mutated?



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Where are promoters usually located?



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In eukaryotic cells, where does the basal transcription apparatus bind?



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