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MCDB 2150 Exam 1 Study Guide What is the purpose of PCR and how does it work? What is an allele and how many do we have of a single sequence? What are short tandem repeats? What is transcription and what is the end result? What enzyme synthesizes RNA on a DNA template? What is the difference between a nucleoside and a nuDon't forget about the age old question of vbs 2032
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cleotide? Where does transcription begin? How can you figure out which strand is the coding strand and which strand is the template strand? What is the region called where RNA polymerase binds? Which direction is RNA transcribed and which direction is the template? What is a codon? What is the difference between unambiguous and degenerate with regard to the DNA code?What is the “wobble” hypothesis? What initiates translation? Where does transcription occur? Where does translation occur? What is the difference between introns and exons? How does the process of alternative splicing work? What are the types of point mutations and what do each of them do? What is a frameshift mutation? How do most mutations arise? Explain the type of mutation that causes sickle cell anemia What is a single nucleotide polymorphism? What is an allele specific oligonucleotide(ASO)? What are restriction fragment length polymorphisms(RFLP’s)?\