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IU / Biology / BIOL 211 / biology 211 exam 1

biology 211 exam 1

biology 211 exam 1

Description

School: Indiana University
Department: Biology
Course: Molecular Bio
Professor: Joseph pomerening
Term: Fall 2014
Tags: DNA
Cost: 50
Name: BIOL-L 211 Exam One Study Guide (Lectures 1-6)
Description: here is a practice exam with answer key to help for the upcoming exam
Uploaded: 02/07/2017
8 Pages 148 Views 0 Unlocks
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SDS helps denature proteins through what mechanism?




A peptide bond within a protein is an example of what type of interaction?




Approximately 44% of the human genome is made up of what type of DNA sequence?



BIOL-L 211 Spring 2017 – Zentner Exam 1 Thursday, February 2, 2017 Choose ONE answer for each question. Each question is worth 2 points. 1. Approximately 44% of the human genome is made up of what type of DNA sequence? A. Transposable elements B. Protein-coIf you want to learn more check out 5.55 as a fraction
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ding genes C. Enhancers D. Non-protein coding genes 2. A peptide bond within a protein is an example of what type of interaction? A. Covalent B. Ionic C. Hydrogen bond D. Van der Waals interaction 3. Which of the following statements best describes Chargaff’s rule? A. The ratios of A:G and C:T in a given DNA molecule are approximately equal B. The ratios of A:C and G:T in a given DNA molecule are approximately equal C. The ratios of A:T and G:C in a given DNA molecule are approximately equal D. All DNA bases are present in equal proportions in a given DNA molecule 4. Which of the following DNA molecules do you predict has the highest Tm? A. ATCGAATATT B. ACCCGATTTA C. GCCTAGCGCT D. AGAATATACC 5. You have discovered a new restriction enzyme that cleaves GAATTC, which is also  cleaved by EcoRI. You new RE is thus an: A. Isomer B. Icosamer C. Isoschizomer D. Isoleucine 6. SDS helps denature proteins through what mechanism? A. Coating of proteins with negative charges B. Coating of proteins with positive charges C. Reduction of disulfide bridges D. None of the above 7. PCR consists of three general steps. Choose the correct order of these steps. A. Denaturation, extension, annealing B. Annealing, denaturation, extension C. Denaturation, annealing, extension D. Extension, denaturation, annealing8. Which of the following DNA sequences is complementary to 5’-ATAACGATAC-3’? A. 3’-TATTGCTATG-5’ B. 3’-ATAACGATAC-5’ C. 3’-CATAGCAATA-3’ D. 3’-GTATCGTTAT-5’ 9. You have isolated a new protein from cat fur that appears to help the fur resist  breakage when pulled. What structural feature do you suspect is responsible for the  protein’s tensile strength? A. Greek key B. Coiled-coil C. β-strand D. β-sheet 10. SDS-PAGE separates protein by: A. Charge B. Size C. Charge and size D. Number of subunits 11. In the context of DNA structure, hybridization and annealing both refer to what? A. Separation of the strands of the double helix B. Interaction of complementary single-stranded molecules to form a double stranded molecule C. Over-twisting of the double helix D. Binding of a protein to DNA 12. Which of the following is true of a covalent bond? A. Can form at many angles B. Very weak C. Act at short distances D. Forms at specific angles 13. You are studying the structure of a mutant protein that has lost a characteristic bend in its structure. What amino acid do you suspect has been mutated? A. Proline B. Tyrosine C. Histidine D. Tryptophan 14. Which of the following short stretches of DNA is most likely to be a restriction  enzyme recognition sequence? A. ATATTC B. GATTAT C. ACGGCA D. GAGCTC15. You have discovered a new folded structure in several proteins and determined that  this structure is stabilized by hydrogen bonds. What level of protein structure does this  new fold belong to? A. Primary B. Secondary C. Tertiary D. Quaternary 16. What is the proper sequence of one-letter designations for the amino acid sequence  methionine-arginine-valine-lysine-tryptophan-glycine? A. MAVLTG B. ARVLWG C. MRVKWG D. MRVKYG 17. An imperfect, nonfunctional copy of a gene is called a(n): A. Pseudogene B. Transposon  C. Intron D. Promoter 18. DNA strands in the double helix are _____________, meaning that they run in  opposite directions. A. Anti-parallel B. Parallel C. Antagonistic D. Complementary 19. Which of the following amino acids contains a ring structure in its side chain? A. Serine B. Glutamic acid C. Cysteine D. Tyrosine 20. In RNA, thymine is replaced by which of the following bases? A. Uracil B. Methylcytosine C. Carboxymethylcytosine D. Inosine 21. Amino acid side chains are also referred to as which of the following? A. Alpha carbon groups B. R-groups C. Amino groups D. Carboxyl groups 22. Which of the following classes of amino acids would most likely be found packed into  the interior of a protein, away from water? A. AcidicB. Basic C. Aliphatic D. Sulfur-containing 23. Retroviruses, in contradiction to the original formulation of the Central Dogma, can  convert their RNA genomes into DNA via what process? A. Translation B. Replication C. Transcription D. Reverse transcription 24. In their studies to determine the nature of the hereditary material, Hershey and  Chase labeled protein with radioactive sulfur. What amino acids were they labeling with  this technique? A. Serine and threonine B. Serine and cysteine C. Threonine and methionine D. Methionine and cysteine 25. What type of molecular interaction holds protein secondary structures such as α helices together? A. Ionic bonds B. Hydrogen bonds C. Disulfide bonds D. Peptide bonds 26. You have isolated a DNA sequence that, when mutated, reduces the expression of  the gene closest to it. However, that “closest gene” is almost 20 kilobases away! Based  on these observations, what type of genomic element do you think your sequence is? A. Promoter B. Silencer C. Operator D. Enhancer 27. A single turn of the DNA double helix consists of approximately how many base  pairs? Due to differing instructions on how to answer this mislabeled question, we  accepted C or D A. 3.4 B. 34 D. 10 C. 20 28. What type of bond forms the backbone of polynucleotides? A. Phosphodiester B. Peptide C. Disulfide D. Hydrogen 29. The 5’ end of a polynucleotide has what chemical group?A. Hydroxyl B. Phosphate C. Amino D. Carboxyl 30. What is the correct sequence of three letter amino acid codes for the peptide  sequence glutamic acid-arginine-asparagine-aspartic acid-glycine-glutamine? A. Gln-Arg-Asp-Asn-Gly-Glu B. Glu-Arg-Asp-Asn-Gly-Gln C. Glu-Arg-Asn-Asp-Gly-Gln D. Gln-Arg-Asn-Asp-Gly-Glu 31. What protein structure is formed by the interaction of multiple α-helices? A. Coiled-coil B. β-coil C. β-strand D. α-coil 32. Chargaff’s rule is linked to what feature of the Watson-Crick model of DNA? A. Base pairing B. Number of bases per helical turn C. Distance between base pairs D. Helix diameter 33. Which of the following genomes has the highest density of protein-coding genes? A. Escherichia coli B. Arabidopsis thaliana C. Homo sapiens D. Drosophila melanogaster 34. A nucleoside is made up of what two molecules? A. A base and a triphosphate B. A base and a sugar C. A sugar and a triphosphate D. A base and an amino acid 35. What are the three major functional classes of RNA in a given cell? A. mRNA, rRNA, snRNA B. mRNA, tRNA, gRNA C. mRNA, rRNA, tRNA D. miRNA, rRNA, tRNA 36. What class of enzymes is responsible for unzipping double-stranded DNA in cells? A. Helicases B. Polymeraes C. Topoisomerases D. Primases37. Which of the following amino acids are basic? A. Tryptophan and leucine B. Arginine and leucine C. Lysine and arginine D. Lysine and tryptophan 38. PCR uses which of the following denaturation methods to separate the DNA strands  of the double helix? A. Heat B. Alkali C. Helicases D. Low salt  39. Point centromeres are found in which of the following organisms? A. Maize B. Human C. Fission yeast D. Budding yeast 40. Which of the following amino acids contains sulfur? A. Methionine B. Serine D. Glycine E. Histidine 41. What is the most common element of RNA secondary structure? A. β-strand B. α-helix C. Coiled-coil D. Stem-loop 42. Which of the following amino acids does NOT have an ionizable side chain? A. Aspartic acid B. Alanine C. Histidine D. Tyrosine 43. The force of attraction between two atoms can be expressed as: A. Chargaff’s rule B. The transforming principle C. Coulomb’s law D. pKa 44. Hemoglobin is an example of a: A. Monomer B. Heterodimer C. Homotetramer D. Heterotetramer45. What class of RNA encodes proteins? A. mRNA B. piRNA C. rRNA D. tRNA 46. A small, independently replicating DNA circle is called a: A. Phage B. Chromosome C. Plasmid D. Clone 47. Human centromeres contain numerous copies of what type of repetitive sequence? A. LINE B. α-satellite C. SINE D. Kinetochore 48. Most bacterial chromosomes are circular and thus lack what features? A. Centromeres B. Telomeres C. Origins of replication D. Repeat regions 49. Disulfide bonds may participate in which two levels of protein structure? A. Primary and quaternary B. Secondary and quaternary C. Secondary and tertiary D. Tertiary and quaternary 50. Which of the following is not an example of functional non-protein-coding DNA? A. Enhancer B. Silencer C. Intron D. Operator EXTRA CREDIT QUESTIONS 51. Why do restriction enzymes degrade phage but not host cell DNA? A. The phage and host cell DNA sequences are different B. The host cell DNA is protected by protein C. The host cell DNA is methylated D. Restriction enzymes are tethered to phage injection sites 52. You are studying a protein that spans a cell membrane. Which of the following amino  acids do you expect to be prevalent in the portion of the protein that is embedded within  the membrane’s lipid bilayer? A. Aspartic acid B. GlutamineC. Isoleucine D. Asparagine 53. Which of the following classes of TE does not encode its own reverse transcriptase? A. TEs with LTRs B. LINEs C. SINEs D. None of the above 54. You have performed a PCR to amplify your favorite gene from your favorite  organism’s genome. When you run some of the PCR out on a gel, however, you see that  in addition to a strong band at the size you expect, you see several weaker bands of  various sizes. How could you increase the specificity of your PCR? A. Decrease the annealing temperature B. Increase the annealing temperature C. Increase the denaturation time D. Decrease the extension time

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