Why make knockout/knock-in mice? Define what CRISPR-Cas9 Technology is. What is the function of Optogenetics?

Name: Neuroscience 3000, Week 5 Notes
Brand: StudySoup
SKU: 2412679
Price: 45.00 USD
Availability: InStock
Rating: 5 (1 reviews)

Neuroscience 3000: Techniques in Cellular Neuroscience A. Transgenic Mice a. Foreign DNA is injected into a mouse germ line i. by injection of one-cell egg or injection of embryonic stem cell into the blastocyst b. New gene added, native gene still present c. Can’t control where the new DNA integrates into the genome d. Can use to express gene in specific cells i. Can determine the effects on the cell or on circuit function e. Overexpress a gene to make a product f. Mark specific cells with GFP (CFP< RFP< YFP), luciferase, Lac Z g. What can transgenic mice be used for? i. Mark specific organelles ii. Trace axons and dendrites with