×
Log in to StudySoup
Get Full Access to OleMiss - BISC 336 - Study Guide - Midterm
Join StudySoup for FREE
Get Full Access to OleMiss - BISC 336 - Study Guide - Midterm

Already have an account? Login here
×
Reset your password

OLEMISS / BISC / BISC 336 / What is polymerase chain reaction?

What is polymerase chain reaction?

What is polymerase chain reaction?

Description

School: University of Mississippi
Department: BISC
Course: Genetics
Professor: Erik hom
Term: Fall 2016
Tags: Bisc336, dr.hom, epigenetics, DNA, RNA, Proteins, Genetics, geneticengineering, Recombinants, recombination, Probability, CRISPR, Cas9, Enzymes, aminoacids, and Studyguide
Cost: 50
Name: Genetics Test #2 Studyguide
Description: BISC 336 Dr. Hom Epigenetics Genetic Engineering Recombinants DNA, RNA, Proteins CRISPR/ Cas9 Enzymes 20 Amino Acids and Traits
Uploaded: 10/09/2017
5 Pages 51 Views 4 Unlocks
Reviews


Studyoguide by: Erica Podocht


What is polymerase chain reaction?



Genetics Test #2, Bisc 336

Dr. Hom

Study soup

Study Soup

Central Dogma tuman home

DNA onomics transcription

RNA - Transcriptomies translation

Protein proteomics

omics: niahlu parallel analysis w) a

Targe of samples exons expressed


What is sanger sequencing?



Don't forget about the age old question of What is the meaning of kouros?

studysound

introns Spliced out

POR Polymerase Chain Reaction

Add Primers Denature-950 doubne Strands separate Don't forget about the age old question of How to approach reconstruction began during the war?

Annualing - 55-65°C

primers anneal 4 extension- 72°C

DNH Synthesis Reaction Mixture

DNK template Fwd+ Rev. primers dNTPs inuele onde building blocks) DNA polymerase (tag on PFU)


What does epigenome mean?



We also discuss several other topics like Worldview means what?

Buffer mixture [+Mq21) target size from POR

*perfect #:

Nn2n-2nn #of rounds

in thermocycor general concepts:

2 (1-2); n 45 (small) Don't forget about the age old question of How are time and space intertwined?

2n in> 5 charge) Sanger Sequencing flourésently labeled ddNTP (dideoxy-) uses primeru polymerase terminator dyes - nudeotides can4 be added Use capillary elechophoresis

w light tosee labeled dNTPs anome sequencing ODNA Sequencing

s. What's the text 2 gene-finding

what does it mean? 6 Annotating We also discuss several other topics like Declaration of independence refers to what?

data/notes about one

- The Human genome was first seguenced

in February Of 2001. -haploid ginome- 3.23 x 10 bp -20,000 coding genes ~ 1.59. Of genome -50% of genome is repecits We also discuss several other topics like What are the conformations of cyclohexane?

210 nucleotide repeats → microsatellite 10-60 nucleotide repeats → minisatellite 2500 nucleotide repeats → SINES

>500 nucleotide repeats - LINES - 14. of lenome are exons that truly

code for protein -.1% varies between individuals epigenomics -DNA modifications (methylation) - histone modifications (aceltulation) -non-coding RNA'S -human body has 10 cells -microbes (mainly bacterial) 1-3x more cells

on the human body

Study Soup

StudySoup

Study Soup

Study Soup

) Study Soup

dy Soup

repeats cas: "crispy associated"

.

double stranded DNA Nickase: only cuts i strand of

the double stranded DNIA;

gene mapping & linkage .

determining *think back

gene function: to Mendel's 2nd postulate,

Oisolate normal gene DNA independent assortment!

o generate mutant allele *remember what happens during

introduce mutant to host

crossing over!

& determine phenotype Recombination might not be a perfect 100%

Retroviruses: Viruses insert ganchic

material into specific DNA Sites - fara part on chromosome = unlinked - the tendency of alleles to be inherited GMOs & Crispr/casas

together frono meiosis is distant dependente - linked <501: unlinked >50%.

GMO: genetically modified Steel

Organism; knock-in gine -ru recombination rate, probability of recomb. CRISPR clustered regularily -M= morgams, cm= 100; expected # of crossover interspaced short palindromic

between two loci, indicates map distance - Map unit-loma 19recombination frequency

casq: proteinlenzyme that cuts - Map function-mapping Mrs. r - "Parental" sum=1-5

"recombinant" Sum=r - Double Crossing over Dco) two cross over areas mutant enzyme

track RNA trans acting crRNA *if independent events, multiply probabilities CrRNI: Crispy RNA, non-coding

! How to Solve Map Problems:

PAM s proto spacer adjacent order data into reciprocal pairs (non-sister chromatids)

recognition 3 look at NCO (parental) - most frequent

dcasq: catalytically dead, has

no cleavage, it just binds 3) look at Dco (double crosses) - least frequent o look at sco (single crosses) two sets + determine distance epigenetics

O reversible chemical modificatie -interference (I)- 1-C

Of DNA - observed # of double recombinants

-methylation hydroxymethylatim

© Chromatin remodeling via expected # of double recombinants

Chemical modification of histones non-coding RNA molecules

-SiRNAs, mRNAs, piRNAs in RNA forward: classical genetics - Start w/ phenotype

*example: methulahon of reverse: Start wl gene, perturbit-phenotype,

agouti gene in mice stud find function of gene

Cancer

p53 gene: tumor surpressor gordas forward mutations: Spontaneous + random,

hyper methylation: suppresses

p ene, randome xray, chemical, insertional

methylated in larea & under more

methylated everywhere else, more reverse mutations: random mutagenesis, targeted

DNA mutation muragenesis, + phenocopying

hupo methylation: fewer methylated sites:

oncogene activation, chromosome instability, high mutation rates

motif; critical for target

Forward + Reverse Genetics

Knock out: inhibition of a gene

) Study Soup

Know how to udyson

Shotgun assemble

- longest overlap sequence read electrophoresis gels

find a mutant gene in a sequence map a chromosome explain a specific research study read the Studies Hom passed

Out in class

Study Soup

or

apply ethics Know CRISPR/Cas9 and how

it works Know PCR & its phases &

how many target Stranda buie were created pey cycle

deamination: makes molecule more

reactable histone modifications:

O acetylation

methylation

phosphorylation s ubiquitination

- Group is added on, common in bio * 207. of histones are positively charged

bic DNA is very negative (backbone is -) chromatin immunoprecipitation (ch IP); Stue

antibodies that recognize histone or

DNA modification Non-coding RNA'S SiRNA

degrades mRNA, inhibits expression,

very specific microRNA

made in nucleus, double stranded,

not specific PiwiRNA

very little known about them, but they function somehow in our

germline ethical Principles

Primany

do ro harm (non-maleficence) do good (beneficence)

do not violate individual liberty (autonomy) @ be fair (justice) Secondary

O tell the truth (honesty)

Kelp promises (fidelity) 6 respect privacy (confidentiality) 4 how much ham should be risked for good?

(risk-benefit analysis) © avoid exceptions (special cases + slippery slope) *know how to apply to a situation

Study Soup

Study Soup

Study Soup

amino acids

StudySoup

Study Soup

Y

OH

NHL

Nit, 'Olt

glycine, 6

honpolar

alanine, A -non-polar -greasy

NH2 valine, v

-non-polar -greasy

glutamic acid, E

Pka=4.1 - acidic (-charge)

-polar

Study Soup

OudySoup

OH

Carboxamide

H N

OH

OH

NH2

4

KY OH o NH2

3 L 8 NH2 aspartic acid, D

Asparagine, N - polar -acidict-charge)

-incharged

glutamine, a soup

Nitz leucine, L

- non-polar

- polar

-polar su

Uncharged

OH

o

oysoup

NH, OH

оние NH2 SOUP threonne, T

он NH2 serine, s

S

Study Soup

isoleucine, I UP-non-polar

- Polar

-polar

Uncharged

uncharged

Cysteine, c - quirky - sulfur atom soup -disulfide bonds

NH

HN

PKG =

2.2

W

ow parts

OH PK4 =1.9

HAN

N.

OH

Studi

OH

u

NH2

(HNINH2

imidazole

aminos NH3 lysine, K

o

histidine, t

NH2 Methionine, M

Arginine, R -quincy

-polar -satom

-basic + charge) - disulfide bonds soup

-polare

basic (+ charge)

-aromatic -singlering

StudySoup

Qlanine

H

HOT

TO CHRYSOU

HO

NH

Phenylgroup

OH

Study Soup

Phenylalnine, F

-aromatic -Single ring

tyrosine, y

- aromatic -singlering

indole group tryptophan, w

-aromatic -double ring

Study Soup

StudySoup

OH

study soup

opka=10.966 Proline, P

- quirky -non-aromatic ring

studySoup

Good luck!

) Study Soup

Page Expired
5off
It looks like your free minutes have expired! Lucky for you we have all the content you need, just sign up here