What is the goal of histological methods? What stain visualize cell bodies only? What is the difference between a CT scan and an MRI scan?

Lectures 8 and 9 Stereotaxic Surgery Sham Group Animals to experience surgery and have cannula inserted; no current or chemical injected (so no lesion); this accounts for incidental damage Histological Methods Goal is to visualize tissue as it was when organism was alive Steps: place brain tissue in fixative (formalin), cut in thin sections, stain and mount sections on slide Called Crystostat Methods rely on freezing the brain so it is hard enough to be cut into thin sections Staining Mouse Brain Nissl Stain visualize cell bodies only (neurons and glia) Doublecortin Stain visualize young neurons- requires immunocytochemistry Immunohistochemistry Antibodies bind specifically to antigens in biological tiss