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Jacksonville State University - BY 323 - Class Notes - Week 1

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Jacksonville State University - BY 323 - Class Notes - Week 1

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background image Microbiology Notes    •  Louis Pasture  -  Saved the brewing industry by having breweries sterilize with boiling  water after so many brews.  -  When there was an anthrax outbreak in a field he used one of his older  cultures of anthrax and injected his herd with it, effectively vaccinating 
-  He used a swan neck flask and left the end open so air would enter and  sterilized the broth in the flask. The broth within stayed sterile until the 
flask was tipped, and the broth was allowed to contact the particles 
trapped in the bend of the flask. Disproved spontaneous generation 
    -  He did the same as he did with anthrax with the rabies virus and made a  rabies vaccine. Russia sent over 100 people to get the vaccine from him.  -  Spontaneous generation – life comes from nothing (just poofs into  existence).  •  Robert Koch  -  Germ theory- germs cause disease 
-  Koch’s postulates: 
1. Bacteria (pathogens) must be present in all cases of disease 
2. the bacteria (pathogen) must be isolated in pure culture and grown. 
3. an individual that has never been exposed to the disease is exposed 
and will have the same symptoms as all other cases of infected individuals.  4. Must be isolated in pure culture again.  •  Edward Jenner:  -Used cow pox to vaccinate people against small pox 
background image Microbiology Notes    - He noticed milk maids would get cow pox when first milking cows and 
they never got small pox. 
•  Microbial structure  -  Bacilli- rod shaped bacteria 
-  Cocci- circular shaped bacteria 
-  Vibrio- comma shaped bacteria 
-  Spirochetes- spiral bacteria 
•  Bacterial arrangements for cocci:    •  Differential stains- they are stains where all types of the bacteria are  stained, but specific types get a specific marker. Examples are gram stains. 
-steps to a gram stain: 
1. Put crystal violet on slide for 1 minute. Crystal violet has a partial 
positive to attract it to the negative charged bacteria. Dyes purple. 
2. Put mordant(iodine) on slide for 1 minute. The iodine enhances the 
crystal violet, so the dye sticks better.  3. put acetone on slide for 10-15 seconds to dissolve the membranes.  This will also take the color out of gram negatives. 

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School: Jacksonville State University
Department: Biology
Course: Microbiology
Professor: Benjamin Blair
Term: Spring 2018
Tags: Microbiology, cells, GramPositive, and GramNegative
Name: Microbiology BY 323 Notes from week 1-2 (minus the ice week)
Description: Notes from class for the first couple of weeks since we had the ice week and no class.
Uploaded: 01/29/2018
5 Pages 28 Views 22 Unlocks
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