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UB / Biomed Engr/Joint / BIO 201LLB / What is the meaning of designing control experiment?

What is the meaning of designing control experiment?

What is the meaning of designing control experiment?

Description

School: University at Buffalo
Department: Biomed Engr/Joint
Course: Cell Biology
Term: Spring 2019
Tags: cellular biology and Biology
Cost: 50
Name: bio 201, cell biology
Description: lab final guide
Uploaded: 05/03/2019
5 Pages 35 Views 1 Unlocks
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Lab exam guideIf you want to learn more check out What is the meaning of bilingual education?

Lab 1 - Designing control equipment.

  1. Independent variable - the cause is suffered as independent variable and is independent of weather they hypothesis is correct or wrong.
  2. Dependent variable - the effect is suffered as dependent variable and it depends on whether the hypothesis is correct or wrong.

We also discuss several other topics like What is the purpose of industrial psychology?

Testing a hypothesis.

Observation.If you want to learn more check out What is the relationship between protein synthesis and gene expression?

Question.We also discuss several other topics like What does the goal- setting theory propose?

Check accumulated scientific data.

Form a testable hypothesis.(finish the hypothesis)

Design an component. (revise the component)Don't forget about the age old question of What is advertising designed to create a positive image of an organization in hopes of drawing in favorable feelings?

Analysis of data gatheredIf you want to learn more check out What are subsistence needs?

Conclusion

communication(publish)

Example -

Hypothesis - deem food is fattening.

Independent variable - deem food

Dependent variable - weight.

Negative control - rules out other alternative explanations. Usually sets elements independent variable.eg. - weight of the people living.

Positive control - positive control is something that affects the dependent variable sequences of some hypothesis being true or false.

Eg. weight of the people with high caloric intake and little calorie burning.

Replication - the process of separating the experiment and rule out the possibility of certain effect of unknown variable.

Lab 2 Biological buffers

  1. mole

1 moles = 6.02 x elementary antics.

(avogadro's number)

Mole =

Eg. what is the mass of 10 moles of

Moles =

Weight = moles x molar mass

= 10 x 18 = 180g

  1. Concentration.

        

Molarity =

        Eg. what is concentration of 10 moles of fructose in 0.5L water.

        Molarity =

        = 20m

  1. pH

pH is the hydrogen ion concentration.

pH = -log[

Acid - pH<7, bases pH>7

Eg. what is the pH of 1m HcL

pH = -log [1]

= 0

  1. Buffers

        

        Buffers are the solutions preventing rapid changes in pH.

  1. Dissociation constant - how much an acid dissociates or base dissociates.

        

        Ka =

HA → M+ + A-

  • Phosphate buffers
  •  =  =
  • Mostly its ion
  • Water is a mixture of hychoney (and hydroxyl (ions
  • Equilibrium: When the concentration of reactants and products is greater than zero and stable
  • pH changes can affect how proteins fold and ultimately changing their structures.
  • Safety precaution - HCl is strong acid
  • Positive control - phosphate buffer
  • Negative control - water
  • PPE: Personal protective equipments
  • pH meter is the special instrument used in the lab.

Lab 3: Brightfield Microscopy

  • Compound Microscopy:

i) 2 magnifying lense -         a) Objective lense

                                b) Ocular lense

ii) eyepiece is the ocular lense. It magnifies and focuses light rays in the plane of retina

  • Objective Lense

        i) Numerical Aperture (NA): It is the measure of how much light a lens can

        gather from a sample. It is best measure of quality of Lense

                N.A.=n(sin)

                n = refractive index

                = ½ of total angle of incoming light

ii) Limit of resolution: Smallest distance between two points that can be distinguished from each other. It is dictated by N.A.

        Abbe equation:

iii) Shorter wavelength, lower limit of resolution, better image quality

iv) N.A is the better measure of image quality than magnification

  • Working distance -

i) Field View - Total area that can be observed at one time. High NA, low field view

ii) High NA, small working distance (Lens must be very close to the sample)

  • Ocular Lense

        i) Total Magnification - Magnification of ocular X Magnification Of objective

  • Substage condenser

↙                ↘

        Condenser lens                Aperture diaphragm

Focus light on sample                Determines angle of cone of light matching                                                 condenser

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