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Practice Questions

by: mythili venkateswaran

Practice Questions CHEM4360

mythili venkateswaran
Texas State
GPA 2.9
Molecular Biology
Dr. Lewis

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About this Document

Practice questions ch 6, 10, 11, 12, 13, 14 and 15
Molecular Biology
Dr. Lewis
Study Guide
50 ?




Popular in Molecular Biology

Popular in Chemistry

This 3 page Study Guide was uploaded by mythili venkateswaran on Sunday September 6, 2015. The Study Guide belongs to CHEM4360 at Texas State University taught by Dr. Lewis in Summer 2015. Since its upload, it has received 52 views. For similar materials see Molecular Biology in Chemistry at Texas State University.


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Date Created: 09/06/15
Recommended Endof Chapter Problems New to the 2nd Edition Molecular Biology Principles and Practice Chapter 6 8 A trinucleotide structure is shown below N NH lt I A N NH2 Problem 68 Molecular Biology Principles and Practice Second Edition 2015 Macmillan Education a As drawn which end of the trinucleotide 5 or 3 is at the top b What is the threeletter sequence of this trinucleotide c Is this trinucleotide RNA or DNA Chapter 10 2 In eukaryotic chromatin approximately what length of DNA is typically associated with a single histone octamer 4 Within the histone structure do protein modi cations occur primarily near the Nterminus or near the Cterminus What features distinguish the structure where modi cations occur Chapter 11 2 A DNA polymerase cannot synthesize a DNA strand de novo from a mixture of deoxynucleotide triphosphates Explain the role of the primer and template in the reaction promoted by this enzyme 7 A key step in the initiation of replication in bacteria is to load the DnaB helicase on to the DNA at the replication origin What proteins function at the replication origin during initiation prior to the loading of DnaB 9 Five DNA polymerases have been identi ed in E coli Two of them DNA polymerases IV and V lack a 3 95 exonuclease activity What is the function of the 3 95 exonuclease activity present in many DNA polymerases How does the absence of such an activity in DNA polymerases IV and V make sense in the context of the cellular function of these enzymes 10 In eukaryotes DNA replication occurs in the S phase of the cell cycle However important preparatory events occur in the G1 phase prior to S phase Describe these events and the status of eukaryotic replication origins as a cell enters S phase 15 The enzyme telomerase is considered a reverse transcriptase synthesizing DNA on an RNA template What is the source of the RNA used in the synthesis of telomeres where is it located and how many nucleotides of the RNA are actually used as template for DNA synthesis Chapter 12 7 What type of mutation is most likely to result from the following lesions a Deamination of cytosine GEC 9 b Formation of 8oxoguanosine GEC 9 c Deamination of adenine AT 9 Chapter 13 5 Describe the three steps that initiate almost all processes related to homologous genetic recombination the recombinational DNA repair of doublestranded breaks Chapter 14 3 If two FRT sites are present in a single DNA molecule in the same orientation the Flp recombinase will promote a deletion of the DNA between the two sites If the FRT sites are altered so that their core regions have a perfectly symmetric palindromic sequence instead of the usual asymmetric sequence how might the fate of the intervening DNA in the reaction change Ignore any intermolecular reactions between the DNA molecules Chapter 15 1 The residues of promoters tend to be rich in A and T residues Suggest why this is so 13 In most organisms specialized DNA repair systems are closely linked to transcription Suggest a biological rationale for this close relationship


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