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by: Renee Lehner


Renee Lehner
GPA 3.73

Merrill Hille

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Merrill Hille
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This 4 page Study Guide was uploaded by Renee Lehner on Wednesday September 9, 2015. The Study Guide belongs to BIOL 401 at University of Washington taught by Merrill Hille in Fall. Since its upload, it has received 25 views. For similar materials see /class/192319/biol-401-university-of-washington in Biology at University of Washington.




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Date Created: 09/09/15
Biology 401 Winter 2008 Study Questions page 1 Lecture 2 Chapter 3 1 There is one important key player that activates both S phase and M phase To understand the cell cycle one must understand how this key player is activated and its function a Describe the enzymatic activity of a Cdk b How does the phosphorylation of threoninelSO by Cdkactivation kinase CAK activate a Cdk and how does the consensus sequence of the Cdk substrate bind to the active site in the Tloop of Cdk c Describe how two other proteins inhibit or activate Cyclin dependent kinases Cdks J The entrance to S phase must be regulated to be sure the cell is ready for replication of the chromosomes and cell proliferation a Describe the function of 2 proteins that block the entrance into S phase b When a mitogen activates S phase GlCdk and G1 S Cdk are first activated How do these activate complexes lead to the activation of S Cdk U It is important that mitosis is entered in an allornone fashion One way is to have the activation be rapid a Describe how MCdk is activated at the end of G2phase b Describe what is synthesized to drive the cell into mitosis and one feed back loop that rapidly increases the activity of M phase Cdk A The anaphase promotion complex functions at many places in the cell cycle For instance it is important for keep S Cdk inactive in G1 phase as well as stimulating the entrance to anaphase and separation of the sister chromatids a What is the enzymatic activity of the anaphase promotion complex APC b What kind of tag does the APC complex add to proteins and how is this addition done c What is the importance of the tag on these proteins d How does a proteasome work Biology 401 Winter 2008 Study Questions page 2 Lecture 3 Chapter 4 5 Double stranded DNA is the basis for the replication of DNA The replication of DNA is however complex because it exists as a double strand with two polar strands running in opposite directions and is extensively packaged as chromatin Finally it is important that the DNA be replicated only once in each cell cycle 0 m 6 CL How does the prereplicative complex block a second replication of DNA during S phase and create the rereplication block When does it bind to the DNA where does it bind on the DNA and what cellular activities are required for its assembly What important proteinenzyme is activated in S phase to initiate replication and what is its general function and specificity Which protein in the prereplicative complex does the signal activate to function in replication Discuss the functions of four enzymes used in replication Explain the statement quotThe formation of an Okazaki fragment is important in copying both strands of the DNA and also in copying the DNA rapidly quot What is the end replication problem that eventually leads to the senescence of cells How is the endreplication problem solved Lecture 4 Chapter 5 and 6 7 A cell must check for complete and accurate replication before it enters mitosis a b What are 2 scenarios that might happen if replication were not complete The cell cleverly binds a protein in the replication fork to check for incomplete replication and then a series of kinase reaction begins Describe how this kinase cascade progresses to block mitosis What protein used in the activation of Mphase cyclin Cdkl cyclinB is finally blocked 8 The goal of mitosis is to separate sister chromatids sequestered in the nucleus What signal drives the cell into mitosis rapidly in an allor none fashion How are the activities of microtubules changed in prophase by this signal How does this activity of microtubules allow sister chromatids to be captured in prometaphase Biology 401 Winter 2008 Study Questions page 3 Lecture 1 Chapter 2 Many model organisms have been prominent in cell cycle research 10 Mammalian tissue culture cells were used to ask what controls the cell cycle In the example below Rao asked what drives a cell into Sphase G1 62 P1 FL r FL I r immediately stays in G2 stays in GZG139 BRIEFS 5 Phase Srphase nucleus phase nucleus S phase nunleus continues DNA emers S phase continues replication according to its replication own timetable Al B C a In A above what protein factor drives the G1 cell rapidly into replication Explain the observation b Explain why the G2 nucleus in B cannot begin replication 0 Explain why the G1 nucleus in C enters the Sphase but the G2 nucleus does no d If you rnixed cells in Mphase with Sphase cells what would happen to the DNA in each nucleus Explain the appearance of the DNA of the S phase cells See lecture 4 e If you rnixed cells in Mphase with G1 phase cells what would happen to the DNA in each nucleus Explain the appearance of the DNA of the S phase cells See lecture 4 Biology 401 Winter 2008 Study Questions page 4 Lecture 1 Chapter 2 Leland Hartwell and Paul Nurse isolated temperature sensitive mutants If the mutant is a temperaturesensitive mutant the cells will grow normally at the normal temperature 25 C but arrest at the higher temperature 36 C 11 If the cells had a mutant in S cyclin the cells would block as shown A PERMISSIVE LOW TEMPERATURE B RESTRICTIVEHIGHlTEMFEHATURE a If the cells had a mutant in cyclin B at which stage would the cells arrest at 36 C How does that happen b If the cells had a mutation in DNA ligase at which stage would the cells arrest at 36 C How does that happen c If the cells had a mutation in APC at which stage would the cells arrest at 36 C How does that happen d If the cells had a mutant in GlS cyclin at which stage would the cells arrest at 36 C How does that happen e If the cells had a mutant in Cdkactivation kinase CAK what would happen to the cells How does that happen You might predict 2 possibilities f If the cells had a mutation in cdc25 at which stage would the cells arrest at 36 C How does that happen


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