Cell and Molecular Biology Lab
Cell and Molecular Biology Lab BS 111L
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Helen Blick Sr.
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Helen Blick Sr.
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This 3 page Study Guide was uploaded by Helen Blick Sr. on Saturday September 19, 2015. The Study Guide belongs to BS 111L at Michigan State University taught by R. Joshi in Fall. Since its upload, it has received 39 views. For similar materials see /class/207329/bs-111l-michigan-state-university in Biological Sciences at Michigan State University.
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Date Created: 09/19/15
LCE Study Guide Rajasi JoshiSection 018 and 022 Knowledge Portion 50 pts in recitation Scientific Method I Given an experimental design tell me What the manipulated dependent and controlled variables are and What the positive and negative controls are I Given a scientific problem and a hypothesis design an experiment for me including proper controls I Know What the difference is between your hypothesis and your experimental predictions Biomolecules I Know the general structures and properties of the biomolecules we worked with sugars starch proteins fats I If I give you an assay name tell me What molecule it tests for I lfl give you a biomolecule tell me What assay you would use to test for it Spectrophotometry I Know the nomenclature AA and ODA ie What do those mean and is there a difference between them I Know and be able to apply Beer s Law I Know how to set the wavelength blank the machine and take a reading of a sample I When given a sample to test tell me What solution would make a good blank for that sample Solutions and Serial Dilutions I Be able to apply the equation CiVi Cfo to make different concentrations of stock solutions Understand how to use X solutions If given a dilution scheme and an initial concentration of a stock solution calculate the concentrations of each dilution If given desired concentrations of a dilution series create a dilutions scheme to create the desired concentrations ie What volumes of diluent and stocks would you use If given the molecular weight of a compound be able to tell me how to make solutions of a specified molarity and volume or percentage and volume Enzymes I Know Why enzymes are affected by environmental conditions e g temperature pH and Why they have optimum temperatures and pHs I Know Whyhow substrate concentration affects enzyme rates and Why the rate max es out Signal Transduction I Know What signal transduction means and does I Know the three stages of signal transduction I Know What a ligand is I Know the roles that GProteins and Tyrosine Kinases play in signal transduction I Know What kinase cascades are and how they affect signal transduction What is their advantage I Know What second messengers are and know examples Paper Chromatography Fly eyes I Know how paper chromatography works I If given chromatography results tell me the order of the biochemical pathway I If given a pathway and mutants predict draw What the chrom atogram would look like Be able to calculate the Rf value for a spot on a chromatography paper PCR Know what PCR does and its applications Know the three steps of a PCR cycle Know what is happening to the DNA at each step of a PCR cycle Know what each ingredient of the cocktail is contributing to the in vitra reaction especially the primers Know why Taq polymerase is used rather than a normal DNA polymerase Transformation What do we mean by lateral transfer of genes What are the various methods of lateral transfer of genes that occur in nature and what are their mechanisms Which lateral transfer method did we used in lab Know what is happening in the transformation tube at each step of the procedure e g What is happening when you incubate at 37 C in LB broth What do we mean by a selective medium Be able to interpret transformation results ie why does the culture grow on one medium but not the other Be able to interpret predict transformation results ie on which media will the culture grow amp on which will it NOT grow DNA Fingerprinting amp Agarose Gel Electrophoresis If given a DNA restriction map and set of enzymes with which to cut the DNA tell me the number and sizes of the resulting fragments If given a gel diagram with the marker lane indicated be able to draw where those fragments would migrate on the gel Be able to compare DNA restriction maps and a gel picture of a digestion to tell me which plasmid was loaded on the gel Know which pole DNA moves toward and why Know the three reasons for adding loading dye to your samples before loading the gel If given a gel picture DNA samples and with marker lane estimate the size in base pairs of the DNA fragments in the samples Bench Skills Portion 20 pts in lab 7 Stations not necessarily in this order 1 Pipetting and Micropipetting 74 min 2 Microscopy 74 min 3 Sening up and loading an agarose gel 74 min 4 Aseptic 1 L 39 39 pram 5 Spectrophotometry 74 min 6 Graphing 7 8 min 7 One or more Rest Stations 7 4 min each Techniques to master 0 Be able to use a micropipenor properly u in are place the comb and L u ueak arru ur ular iu our case on an agarose gel 0 Be able to successfully load wells in an agarose gel 0 Be able to focus a microscope 0 Be able to streak an agar plate to obtain isolated ie pure culture colonies 0 Be able to properly perform the spread plate technique D b a elm rlrselectablank vv sp ectrophotometer 4 4 a sample using a 0 Be able to use Excel to make and label a graph from a given set of data Graphing 0 Know what a standard curve is and what it is used for remember not all graphs are standard curves 0 When given a data table be able to use Excel sorry to graph the data points 0 Be able to lable the axis properly 0 Be able to set the scale ofan axis linear or logarithmic 0 Be able to set grid lines properly both horizontal and vertical 0 Be able to the graph a proper title 0 Be able to create a bestfit line 0 Be able to display the equation for that line 0 Know semilog Vs regular graph scales Examples Example Graph 1 mm mm mmquot mmmmmqymnmxm m w nunmap mm m mm mum mm A man 2n Mmmmimnm 50 Example Graph 2 mm Dilemllnillwnol Optimum nmmrm or mwumm am a 5mm 50 20m 250 mu Temwlrllwe New mm
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