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by: Alvah O'Conner IV


Marketplace > Iowa State University > Biology > BIOL 212L > PRIN BIOLOGY LAB II
Alvah O'Conner IV
GPA 3.79


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This 1 page Study Guide was uploaded by Alvah O'Conner IV on Sunday September 27, 2015. The Study Guide belongs to BIOL 212L at Iowa State University taught by Staff in Fall. Since its upload, it has received 79 views. For similar materials see /class/214536/biol-212l-iowa-state-university in Biology at Iowa State University.




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Date Created: 09/27/15
Biol 212L Neural and Sensory Systems Student Guide I Nervous System Microanatomy work individually Dl Study a prepared slide showing a smear of cells from a spinal cord using a compound microscope Look through the smear to identify neurons and glial cells and the structural differences between these cell types Look for axons or dendrites and formulate a reason that these are hard to distinguish in this slide D2 Study a spinal cord crosssection prepared slide following instructions in your lab manual D3 neuromuscular junction prepared slide compound microscope Identify muscle cells axons of motor neurons and neuromuscular junctions which are synapses between motor neurons and muscle cells Examine a cross section of the retina of an eye prepared slide compound microscope Use Fig 3210 in your lab manual as a guide Try to identify the layers of structures 7 blood vessels on the outer surface then layers of nerve cells then sensory cells rods and cones are probably not distinguishable Cleanup Return all slides to the correct slide tray 7 do not leave slides on microscope stages You will use microscopes again near the end of the lab II Pig nervous and sensory system dissections D4 5 Spinal cord dissection fetal pig lab groups Use fetal pigs with body cavity organs removed Dorsal dissection to reveal meninges dorsal and ventral roots spinal nerves At each table decide which group will dissect the cervical thoracic or lumbar region When your dissection is complete exchange with other groups so you get to view all three regions Look at the spinal region of your pig from inside the body cavity to see sympathetic trunks amp spinal nerves D6 7 Brain dissection Identify brain regions and cranial nerves using your lab manual as a guide Making sketches is a good way to reinforce learning Fetal pig brains are small and soft and difficult to expose and remove without damaging A skillful group may be able to do this successfully so feel free to test your skills and the quality of your pig Each table will also have one adult pig brain to examine Handle adult brains with care and do not do any unnecessary dissection or damage so that they can be used by other groups if necessary D8 9 Eye dissection work in pairs Follow instructions in your lab manual to identify major structures of a mammalian eye We39ll use eyes from adult sheep Cleanup This the last time you will use your fetal pig Check with your TA to find out if you should save any specimens especially brains If not all animal tissue goes in the tissue waste container Your TA will tell you where to put tissue waste and paper and plastic waste Clean and wipe desktops if any uids are present Wash and dry dissecting tools and rinse dissecting trays III Stretch re ex 7 demonstration Be certain you understand the neural wiring that underlies a stretch reflex and the physiological significance of the reflex response IV Visual system demonstrations blind spot and afterimages Understand why we have a blind spot and why we are not usually aware of its existence D 10 Color vision Understand how your eyes discriminate between colors and how that mechanism is related to the formation of colored afterimages V Auditory system lab work Careful dissection of the middle and inner ears are impractical in the time available D 12 Use models for ear anatomy D 13 Organ of Corti prepared slide Warning preparation artifacts often distort cochlear structures The tectorial membrane may be folded away from the basilar membrane


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