4010: Biochemistry and Molecular Biology I - Study Guide
4010: Biochemistry and Molecular Biology I - Study Guide 4010
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This 6 page Study Guide was uploaded by Addison Sanders on Friday October 17, 2014. The Study Guide belongs to 4010 at University of Georgia taught by Glover in Fall. Since its upload, it has received 147 views. For similar materials see Biochemistry and Molecular Biology I in Biochemistry at University of Georgia.
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Date Created: 10/17/14
BCMB 40106010 Lectures Fall 2014 Day 28 1 Test 3 2 Format a Emphasis on my lectures and handouts but I will likely ask at least one question from Garret and Grisham that I did not discuss in class I want you to read the book b A mixture of truefalse multiple choice short answer and problems c See sample tests on electronic reserves and eLC 3 Things to Memorize Carbohydrates a Carbohydrate definition b Carbohydrate nomenclature 1 aldoses ketoses 2 trioses tetroses etc 3 monosaccharides disaccharides etc 4 glycoproteins glycolipids etc c Carbohydrate stereochemistry 1 DL configuration 2 enantiomers diasteriomers epimers anomers d Carbohydrate cyclization 1 hemiacetal hemiketal 2 furanose pyranose 3 on and 3 anomers 4 mutarotation e Carbohydrate conformation 1 envelope twist chair boat 2 equatorial Versus axial f Monosaccharide derivatives 1 aldonic acids uronic acids alditols deoxy sugars amino sugars 2 acetals and ketals glycosides and ketosides g Monosaccharide structures Haworth projections 1 glucose glucuronic acid glucosamine GlcNAc 2 galactose mannose fructose ribose also know the abbreviations for the above h Disaccharide and polysaccharide structures Haworth projections Disaccharide Polysaccharide 1 lactose 2 sucrose 3 maltose starch glyco gen 4 cellobiose cellulose 5 chitin also know the shorthand notations for the above i Three dimensional structure properties and degradation of 1 starch glyco gen 2 cellulose Cell Surfaces a Cell surfaces 1 prokaryotic a cell wall 2 eukaryotic a glycocalyx b extracellular matrix b Prokaryotic membranes and cell walls 1 Gram positive and Gram negative bacteria 2 peptidoglycan Gram positive and Gram negative 3 teichoic acid Gram positive 4 lipopolysaccharide Gram negative Lipids c Glycoproteins 1 O linked linkage 2 N linked linkage and core a high mannose complex hybrid b biantennary triantennary etc d Proteoglycans 1 glycosaminoglycan chains 2 structure and function of typical proteoglycans including cartilage e Cell surface structures 1 peptidoglycan 2 core oligosaccharide of N linked oligosaccharides 3 hyaluronic acid a Lipid definition b Lipid classification c Fatty acid structure nomenclature and notations d Effect of cis trans unsaturation on tail geometry melting temperature e Triacylglycerols fats and oils f Glycerophospholipids 1 stereochemistry sn nomenclature 2 headgroups 3 ether glycerophopholipids g Sphingolipids 1 sphingosine ceramides 2 sphingomyelins 3 glycosphingolipids cerebrosides gangliosides h Eicosanoids 1 precursor arachidonic acid 2 quotcyclicquot pathway prostaglandins thromboxanes 3 quotlinearquotpathWay leukotrienes i Waxes j Terpenes 1 isoprene 2 nomenclature monoterpene sesquiterpene etc k Archaebacterial GDGTs l Steroids m Lipid structures 1 fatty acids including numbering and lettering schemes a myristic palmitic stearic b oleic 2 triacylglycerols a tristearin triolein 3 glycerophopholipids a phosphatidic acid b other headgroups phosphatidylcholine serine ethanolamine 4 isoprene Membranes a Lipid monolayer micelle bilayer 1 permeability of phospholipid bilayer b Biological membranes uid mosaic model 1 composition phospholipid cholesterol protein carbohydrate 2 thickness X ray diffracton a core hydrocarbon peripheral hydrocarbon and headgroup regions b dimensions 12 2 X 09 30 nm 30 2 X 06 42 nm 3 lateral and transverse mobility of lipid and protein 4 lateral and transverse asymmetry of lipid and protein 5 phase transition melting temperature a gel solid ordered So state b liquid crystal liquid disordered Ld state 6 phase separation of membrane lipids and membrane rafts c Membrane proteins 1 definition of peripheral and integral 2 classes of integral membrane proteins a single OLl 1CllX b multiple on helices c large 3 sheet barrel d monotopic 3 membrane protein topology a hydropathy plots 4 lipid anchors a N myristoylation S acylation prenylation GPI Membrane Transport a Membrane transport mechanisms and characteristics 1 passive diffusion simple diffusion 2 channels and pores 3 passive transport facilitated diffusion 4 active transport a primary b secondary b Transporters 1 uniport symport antiport 2 electrogenic versus electroneutral c Free energy difference across a membrane 1 for an uncharged species a chemical potential difference b AG RT ln C2C1 2 for a charged species a electrochemical potential difference b AG RT ln C2C1 ZFA I1 d Kinetics saturable versus nonsaturable e Passive diffusion f Channels and pores 1 potassium channel and ion selectivity 2 porins g Facilitated diffusion 1 alternating conformation model h Primary active transport 1 transport processes driven by ATP a Na K ATPase b Ca2 ATPase c ABC transporters and multidrug resistance 2 transport processes driven by light a bacteriorhodopsin i Secondary active transport 1 ion gradients in various organisms 2 symport and antiport j Omitted sections of Chapter 9 1 quotThe Gastric HK ATPasequot 2 quotBone Remodeling by Osteoclast Proton Pumpsquot 4 Things to Know How to Do a Given the systematic name of a saccharide give the structure and vice versa b Interconvert Fisher linear Fisher ring and Haworth projections using curly arrows c Draw a hexose in its most stable chair conformation d Draw the mechanism of acetalketal formationhydrolysis oxonium ion intermediate e Give the systematic name and symbol of a lipid given the structure and vice versa f Decompose a terpene into individual head to tail and head to head isoprene units g Calculate a diffusion distance given the equation a diffusion constant and time h Calculate the free energy difference across a membrane 5 Miscellaneous a Pencil or pen is fine b The gas constant and Faraday39s constant will be provided c Bring a calculator
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