Midterm Study Guide
Midterm Study Guide Biol 2259 L02-L03
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This 6 page Study Guide was uploaded by Camille Alvin on Thursday October 8, 2015. The Study Guide belongs to Biol 2259 L02-L03 at University of North Carolina - Charlotte taught by Melanie Harris in Fall 2015. Since its upload, it has received 176 views.
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Date Created: 10/08/15
Microbiology Study Guide 2 or more stains Gram 1 Crystal violet 2 Iodine 3 Alcohol 4 Safranin Endospore Stain Costridum and Bacillus Vegetative Cells red Endospore green AcidFast Stain acidfast and non acidfast bacteria Acidfast mycolic acid waxy Red Non acidfast Bue Clinically Important genera mycobacteria infectious diseases tuberculosis resistant to antibiotics Unicellualr yeast Multicellualr molds Aspergillus Penicillin Rhizopus Motility 1 Cilia Paramecium 2 Amoebid Amoeba 3 Flagella 4 No motility Paramecium Amoeba enriched and differential Hemolysins toxins that break down RBC s Differntiate hemolysis 1 Beta complete breakdown of RBC a Clear halo effect 2 Alpha partial breakdown a Greenishpartially clear 3 Gamma no lysis intact blood agar a Clear selective and differential Selective high salt Halophilic salt tolerant Staphylococcus only can grow 1 Pathogenic grow and also ferment mannitol to acidphenol red yellow 2 NonPathogenic grow but cannot ferment mannitol pink seective and differential Selective dyes eosinmethylene select for gram coliforms Coliforms lactose fermenting gram rods Differential lactose fermentation differentiates between coliforms fermenting and noncoliforms nonfermenting Coliforms acid trigger to pink E Coli metallic green Noncoliforms colorless seective and differential Selective bile salt and crystal violet for gram coliforms Differntial lactose fermentation Coliforms pink Noncoliforms colorless Nosocomial hospital acquired infectionsillnesses Ventiator Associated Pneumonia VAP Causes Gram Streptococcus Staphyococcus Gram coiforms E Coli Noncoiforms Pseudomonas ony plates with 30300 colonies are validcountable Hydrogen Peroxide toxic broken down by catalase to form water and oxygen Distinguishes gram cocci from another Staphyococcus catalase Enterococcus catalase Cytochrome Oxidase nal enzyme in ETC reduces oxygen to form water Ex Cytochrome C Oxidase Purpose Discover whether Cytochrome C Oxidase is in ETC Will have gram rods Aerobic Pseudomonas Oxidase Facultative Anaerobes E Coli Oxidase E a l ul f i i i i lTl Eact al Tuba M Emilia Medium Expl mliin If Inmh Fairlyme as h igat w I nlgI amuih pmMET xyg mquim rMIi mum u y mere high cancantralfiun ul39 mmEH ham i usec mm the Lmazi ium L Amm a n H r h z I m Duly WEE minis mm li39i Ml t i 39 ism EJHE EIEHEIE Elf nxylerri r niy amamim lgsrwth n mammal I Hagan Elam ambit ang f hi mm greater HIEWm in magmaE I39 swam Eimwih mum srnl y Where mam f5 rm WWW Emmii is has 51 WhEFL39 M14351 myg n is WHEEFEL but GGEJ PS mmmzj lmuft tuba Emma mind Batman has nu efi mg
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