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by: Stuart Dach


Marketplace > Oregon State University > Botany > BOT 350 > INTRODUCTORY PLANT PATHOLOGY
Stuart Dach
GPA 3.96


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This 3 page Study Guide was uploaded by Stuart Dach on Monday October 19, 2015. The Study Guide belongs to BOT 350 at Oregon State University taught by Staff in Fall. Since its upload, it has received 40 views. For similar materials see /class/224488/bot-350-oregon-state-university in Botany at Oregon State University.




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Date Created: 10/19/15
BOT350550 Practical Study Guide Isolation of Fungal Pathogens I Pathogens with which life strategy e g facultative parasite are incapable of growing on culture media Wh I What is the purpose of surface sterilizing infected plant tissue before culturing it I Why isolate pathogens from the interface between healthy and diseased tissue rather than from the diseased tissue itself I What is selective media What is differential media Remember that media can be both selective and differential I Why are antibiotics commonly added to isolation media for fun i I What special technique is often used to isolate soilborne Oomycetes Why Koch s Postulates I What is the purpose of Koch s Postulates I What are the four steps of Koch s Postulates Note Determine the pathogen is not an acceptable answer for postulate one Oomycetes Water Molds I now two features that distinguish Oomycetes from true fungi I Know oospore oogoniumantheridium zoospore and sporan ium I Which of the above structures are asexual Which are sexual or the direct product of sexual reproduction I Which of the above is a resting or overwintering structure I Know how to recognize sporangia and oospores under the compound microscope I Why does wet poorly drained soil often contribute to the establishment and spread of Oomycete diseases I Diseases white rust downy mildew late blight of potato damping off Ascomycetes Sac Fungi I o Ascomycetes have septate or aseptate hyphae I Are they predominately haploid diploid or dikaryotic I Know ascospores ascus ascocarps cleistotheciumascoma perithecium apothecium sclerotium stroma conidia conidiophores separate synnema sporodochium asexual fruiting bodies acervulus pycnidium I Which of the above are sexual structures Which are asexual structures Remember that regular or teleomorphic ascomycetes have both sexual and asexual structures while asexual or anamorphic ascomycetes have only asexual structures I Which are overwintering structures I What is an anamorph What is a teleomorph I How many ascospores does an ascus contain I Powdery lLi1dews have which of the three ascocarps I What three traits are used to key out powdery mildews I Be able to recognize asci cleistothecia perithecia stroma conidiaconidiophores and asexual fruiting bodies under the compound microscope I Diseases eastern filbert blight white mold tar spot of maple apple scab ergot powdery mildew grey mold early blight Vem39cillium wilt Basidiomycetes Club Fungi I Are Basidiomycetes predom inater haploid diploid or dikaryotic I Are they septate or aseptate I Know basidiospores basidia naked or in basidiocarps basiodocarps teleospores telium sperm atia receptive hyphae spermagonium uredospores uredium aeciospores aecium I Which of the above structures belong to ascocaIp basidiomycetes Which belong to rusts Which belong to smuts I Which of the above spores is the direct product of sexual reproduction Which of the above are overwintering structures I For some rusts the only functional spore is the uredospores how does a rust of this type overwinter I Be able to identify basidia with basidiospores uredospores in uredia and teliospores in telia under the comound microsco e I Define macrocyclic microcyclic heteroecious autoecious I Are smuts monocyclic or polycyclic I Diseases rusts smuts wood rotsdecays Bacteria I What are five methods used to diagnose bacterial diseases and identify bacteria I Define peritrichous and polar agella I Know the basis of the gram stain test and the colors of gram positive and negative bacteria I Know the basis of the hypersensitivity response when testing for pathogenic bacteria including the importance of the type III secretion system I What characteristic of bacteria does the oxidationfermentation test investigate I Diseases soft rot and ring rot of potato fire blight crown ga Viruses I What are five methods used to diagnose viral diseases and identify viruses I Know the basics of how an ELISA test works I What is the difference between a local lesion host and a systemic host when conducting a viral pathogenicity bioassay How do the two differ in resistance I Diseasessymptoms chlorotic mosaics Tobacco Mosaic Virus leaf curls vein clearing stunt syn omes Botrytis Predisposition I Know why Baz ryl is is considered an example of a weak pathogen ie know its life strategy I Why would the addition of rose petals or potato dextrose broth aid in the establishment of Barrylis Race Identi cation of Wheat Strip Rust I Define race and differential cultivar I Do necrosis and chlorosis in wheat cultivars indicate susceptibility or resistance to a particular rust race I How do rgenes fit in Biocontrol of Crown Gall Know the importance of both Agrabacten39um radiabacter and Agrabacten39um tumefaciens in the biocontrol experiment I What is the importance of the production of agrocin inA radiabacler K84 I How does A tumefaciens produce galls in host plants N em atodes I There are ten characteristics commonly used to identify nematodes know five I What is the importance of the stylet in plant parasitic nematodes I What is the difference between endoparasitic ectoparasitic sedentary and migratory nematodes I What are two methods used to extract nematodes from roots or soi I Be prepared to key out or identify nematodes Electron Microscopy The electron microscope is especially useful for viewing which major pathogen group I What differences are there between transmission electron microscopy TEll and scanning electron microscopy SENT I What are positive and negative staining in TEM


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