LabQuiz2StudyGuide.pdf BIO 100
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This 3 page Study Guide was uploaded by Dylan Notetaker on Monday October 19, 2015. The Study Guide belongs to BIO 100 at Eastern Kentucky University taught by Dr. Brammel in Fall 2015. Since its upload, it has received 32 views. For similar materials see Intro to Biology in Biology at Eastern Kentucky University.
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Date Created: 10/19/15
Dylan Sturgell 1 Lab Quiz 2 Study Guide Difference between Aerobic and Anaerobic Respiration 1 Aerobic Respiration requires Oxygen 02 a C6 H1206 602 gt 6H20 ATPS b Glycolysis i Glucose 9Pyruvates ii Occurs in the cytoplasm c Citric Acid Cycle Kreb s Cycle d Electron Transport Chain i Pyruvates 9 2 3638 ATP energy ii Also releases C02 and H20 e Citric Acid Cycle Kreb s Cycle and Electron Transport Chain occur in the mitochondria Aerobic respiration yields 3638 ATPs g Produces CO2 and H20 2 Anaerobic Respiration occurs in the absence of Oxygen a Fermentation 1 C6 H1206 gt Ethanol 1 C02 2 ATP ii C6 H1206 gt Lactic acid C02 2 ATP iii Glycolysis 1 Glucose 9Pyruvates iV Pyruvates 1 9 Lactic Acid 2 Or 9 Ethanol b Anaerobic Respiration yields 2 ATPs c Produces Lactic Acid and Ethanol With CO2 Process of Photosynthesis 1 Photosynthesis takes place in the Chloroplast a 6C02 6H20 gt light energy gt C6 H1206 602 2 Only in Plants 3 Various photosynthetic pigments a Chlorophyll A b Chlorophyll B c Carotenes d Xanthophyll ReVieW of Fermentation experiment Dylan Sturgell 2 1 Tube A Water and Yeast a Did not produce any gas because there was nothing for the yeast to eat 2 Tube B Yeast and Glucose a Produced C02 gas because it had all the ingredients necessary for anaerobic respiration in yeast 3 Tube C Yeast Glucose and NaF a Had all the necessary ingredients like Tube B but we added NaF a cell poison that killed the yeast Difference between Genotype and Phenotype 1 Genotype a Genetic makeup of organism b Represented by letters EE Ee FF Dd etc 2 Phenotype a The appearance of the organism b Physical description c Red Blue Tall Short Dimples no Dimples etc Difference between Homozygous and Heterozygous 1 Homozygous a Two copies of the same allele EE or ee b Can be Recessive or Dominant 2 Heterozygous a One copy of each allele Ee b Can ONLY be Dominant Review of process used to find the murderer in Lab 7 1 4 microliters of sample placed into each well of Electrophoresis Chamber 2 Connected the negative charge to the top of lanes and positive charge to the bottom of lanes 3 Turned on power source The DNA moves from negative end to positive end 5 DNA made of nucleotides a Repetitive sequences are called Short Tandem Repeats STRs P What are the names of equipment used and how do they work 1 Micropipette a Press plunger to uptakeeject liquid b Adjusts from 05 9999 microliters Dylan Sturgell 3 c Twist plunger to adjust 2 Electrophoresis Gel a Samples from the restriction enzyme digests are introduced into the gel b Electric current is applied causing fragments to migrate through the gel c 10 wells d Only 8 wells are used i 2 outside wells are not used 3 Electrophoresis Chamber a Negative Where the sample is submitted b Positive Where the sample is drawn to c DNA is slightly negative i Opposites attract How do you make large quantities of DNA in the Lab 1 DNA can be replicated with PCR methods a Polymerase Chain Reaction i Sample heated to denature DNA ii DNA is cooled to a lower temperature to allow annealing of primers iii DNA is heated to 72 celcius the optimal temperature for Taq DNA polymerase to extend primers iv After 20 cycles a single fragment produces over one million copies
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