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Introduction to Developmental Biology

by: Mrs. Lester Zboncak

Introduction to Developmental Biology 002 104

Marketplace > University of Iowa > Biology > 002 104 > Introduction to Developmental Biology
Mrs. Lester Zboncak
GPA 3.7


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This 4 page Study Guide was uploaded by Mrs. Lester Zboncak on Friday October 23, 2015. The Study Guide belongs to 002 104 at University of Iowa taught by Staff in Fall. Since its upload, it has received 45 views. For similar materials see /class/228034/002-104-university-of-iowa in Biology at University of Iowa.


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Date Created: 10/23/15
002104 Introduction to Developmental Biology Study Guide 111 This handout is an outline of the material covered in the discussion of gastrulation and the subsequent differentiation of the three germ layers The material is from portions of Chapters 67 and 9 Anything discussed in lecture is fair game If you work through all the concepts and understand the details of the experiments and their results you should do well on the exam 1 Gastrulation general concepts from Chapter 5 and lecture What is the end result of this stage How do the blastomeres change position and why is this important De ne the 3 primary germ layers and their positions relative to each other Understand that the mode of gastrulation varies with yolk quantities State differences of gastrulation among isolecithal moderate telolecithal and telolecithal eggs What is meant by tube within a tube body plan 2 Cell Movements from Chapter 5 and lecture Describe and draw the basic cell movements involved in gastrulation Which 3 types of movements involve expansion on the surface Which 3 types are responsible for displacement to the interior Note evidence for cytoplasmic factors controlling migration Lillie Expt Ingression de ne the sea urchin primary mesenchyme Understand interactions of these cells with the extracellular matrix Describe at least 4 components are in the extracellular matrix ECM 3 Amphibian frog Gastrulation Chap 5 lecture and lab How does amphibian gastrulation dilTer from sea urchin gastrulation hint cell number and yolk content make it more complex What is a Fate Map Be able to define dorsal ventral gray crescent Explain the Vital dye technique and how it can be applied to fate mapping Where is gastrulation initiated in the frog and what is the marginal zone Define blastopore archenteron bottle cells yolk plug How do the vegetal cells initiate buckling what cellular component is involved What cells invaginate first and what do they become What is chordarnesoderm and when do these cells involute 4 Dorsalventral axis Chap 5 lecture and lab How does the blastopore lip change during gastrulation What determines the position the blastopore in normal fertilization Understand the importance of the cortical rotation Experiments disrupting this cytoplasmic rotation How does this correlate with the dorsalventral axis How can you experimentally induce 2 sites of gastrulation Describe the experiments that localize the gastrulation inducing factors Is the ability of bottle cells to initiate gastrulation an innate property difference between frogs and salamanders 5 Mechanics of gastrulation Chap 5 and lecture Understand the importance of the ECM and blastocoel roof in migration What is fibronectin know the structure and two functions Experiments showing the importance of the blastocoel roof in involution Salamander injection expts Excised blastocoel roof expts Outline the orchestration of events during frog gastrulation overview 6 Mammalian Gastrulation Chap 5 and lecture Define primitive streak Origin of hypoblast and epiblast tissues and what do they become Compare to chick gastrulation Define Hensens node be able to outline general cell movements Know Figure 636 First differentiation ICM vs Trophoblast during cleavage stage Followed by further differentiation to generated germ layers and support structures Cytotrophoblasts Syncytiotrophoblast Karyokinesis hormone production 7 Neurulation Lecture and chap 7 What are Von Baer s four generalizations What is organogenesis and how is it initiated Be able to diagram and describe the events of this stage Define Neural plate neural groove neural crest Describe the 4 distinct stages and the mechanics involved formation interaction with underlying mesoderm shaping microtubules and microfilaments bending intrinsic and extrinsic forces closure Importance of Ncadherin in the separation of neural tube frog expt 8 Neurulation in Humans Lecture and chap 7 Evidence for neural tube closure initiated in several places spina bi da anecephaly 9 Differentiation of Neural tube Lecture and chap 7 Overview anatomical level tissue level and cellular level What is ballooning and what causes it What is occlusion where and when does it occur expt in chick Formation of the brain regions g 78 What region generates segmental pattern and expt Determining developmental fates chick 10 Tissue Architecture of the Central Nervous System Lecture and Chap 7 What is the CNS and what is it s function Generation and differentiation of the layers De ne mantle marginal ependymal white matter gray matter Correlate cell cycle with position in the neural tube Cerebral organization general idea migration differential growth and cell death 11 Neural Migration Lecture and chap 7 De ne glia neuron neuroblast Glial guidance 4 steps What is the major protein involved in adhesion experimental disruption of function antibodies What are weaver mice Explain their defect and phenotype 12 Neuronal types Lecture and chap 7 Sketch a typical motor neuron and label structures What are the functions of the axon soma dendrite myelin sheath What is a neurotransmitter Identify 3 types What is the Autonomic Nervous system what does it control sympathetic vs parasympathetic system antagonism neurotransmitters involved Somatic Nervous System 2 types of nerve bers sensory and motor outline functionslocations 13 Peripheral Nervous System lecture and chap 7 What is the PNS what is it s function What is the PNS composed of describe placodes and what they give rise to 14 Neural Crest NC Cells lecture and chap 7 What are they where are they derived Involvement of Ncadherin in their migration List the Cell types of the cephalic NC trunk NC Neural Crest Migration in Chick and Quail Outline NC graft experiments Are the cells pluripotent What is pluripotent Paths of Migration Ventral and the involvement of sclerotome EXpts Manipulating somite number and NC migration Dorsolateral path 15 Epidermis lecture and chap 7 generation of layers de ne periderm basal layer spinous Diagram the layers of the human epidermis What comprises the Malpighian layer what are the characteristics of these cells What is keratin Where do melanosomes come from What is TGFoc and what is its function What is psoriasis and what is this disorder linked with Outline the Transgenic mice with activated TGFoc eXpt What structures are derived from the epidermis


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