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Microbial Diversity and the Biosphere

by: Mrs. Willis Mante

38

0

3

Microbial Diversity and the Biosphere MCDB 4350

Mrs. Willis Mante

GPA 3.6

Staff

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COURSE
PROF.
Staff
TYPE
Study Guide
PAGES
3
WORDS
KARMA
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Popular in Molecular, Cellular And Developmental Biology

This 3 page Study Guide was uploaded by Mrs. Willis Mante on Thursday October 29, 2015. The Study Guide belongs to MCDB 4350 at University of Colorado at Boulder taught by Staff in Fall. Since its upload, it has received 38 views. For similar materials see /class/231843/mcdb-4350-university-of-colorado-at-boulder in Molecular, Cellular And Developmental Biology at University of Colorado at Boulder.

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Date Created: 10/29/15
Vignette Poisson Statistics 1 Poisson Statistics describes distributions of discrete and small numbers It is often useful in biology and in thinking about the set up and interpretation of experiments You probably will see a problem similar to these on Midterm I 2 P probability of event Q probability of non event PQ 1 A Remember also that probabilities multiply Pxy Px Py 3 For small discrete numbers x m x Px 6quotquot Where x number of events m mean number of events Px probability of x events 4 This expression is useful for all sorts of experimental considerations and problems eg Problem 1 You are interested in determining the quotburst size of a bacteriophage growing on E coli In order to do that you mix suspensions of phage and E coli as follows 1 ml Ecoli at 5x108ml 20 pl phage at 25x1010ml What portion of the bacteria are infected with exactly one phage Two phage Three phage What portion of the bacteria are uninfected Ie what is P0 Remember here that by convention n0 1 and 0 1 so PO J Continuing with the experiment you dilute the infected culture so that when you put 100 pl into different wells of a multiple well plate you are putting on average In one bacterial cell per well you will incubate to lysis and then assay the number of phage in the indvidual wells to extract burst size In the distribution into wells What fraction of the wells gets exactly one cell What fraction of the wells gets one infected cell Problem 2 What would the phage bacterium ratio have to be so that exactly 95 of the bacteria are infected Problem 3 You are engaged in a genome project and are dancing between costs and maximum coverage You choose a random approach If you sequence single pass one sequence run on a one strand genome equivalent on your library what would be the coverage fraction nucleotides sequenced single pass How many passes for 95 double strand coverage a common goal Problem 4 Make up and solve a problem using Poisson statistics Poisson Problems Solved 1 One ml Eco at 5X108ml 5x108 cells 20 page at 25X1010ml 5x108 phage m 1 phage per cell a Cells with exactly one phage P1 111le39m e391 037 remember that e391 o37 b Cells with exactly two phage P2 122l1e391 e3912 018 0 Cells that are uninfected P0 10ol1e391 e391 037 remember N0 10 1 d Distribute into wells m1 Pone cellwell 037 Pone infected cell 037X037 014 2 m for Pinfection 095 Flip the question P0 005 6quotquot m3 3 Singlepass means m1 a Psequence for any nt e39m 037 b Double vs singlestrand coverage hinges only on the number of nucleotides covered For 95 coverage as above P0 005 6 m 3 For 95 double strand coverage m twice single pass 6

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