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BIOC405 Exam
Study Guide
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This 8 page Study Guide was uploaded by Andrew on Sunday November 22, 2015. The Study Guide belongs to 406 at DCH Regional Medical Center taught by Hurley in Fall 2015. Since its upload, it has received 29 views. For similar materials see Biochemistry in Chemistry at DCH Regional Medical Center.


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Date Created: 11/22/15
Biochemistry Exam 1 VERSION B October 23, 2015 Instructions: 1. BE SURE TO PUT YOUR NAME, STUDENT NUMBER, AND EXAM VERSION (B) ON THE ANSWER SHEET . 2. Mark the answers on the answer sheet. 3. No books or notes are to be used during the exam. 4. 4. No calculators are to be used during the exam. 5. The test consists of 26 questions. 6. Each question has one correct response. 7. Each question is worth the same number of points. 8. This is a 50 minute exam. Biochemistry 405 Exam 1 VERSION B October 23, 2015 1) Of the various types of metabolic control we discussed in class, which one of the following statements is correct for glycolysis? A. Glycolysis uses only allosteric regulation and global regulation at the level of the organ. B. Glycolysis uses only covalent modification and allosteric control. C. Glycolysis uses only covalent regulation and transcriptional regulation. D. Glycolysis uses only global regulation at the level of the organ and covalent modification. E. All of the above-mentioned types of regulation occur in glycolysis. 2) Which one of the reactions of the citric acid cycle listed below is not reversible under physiological conditions? A. the reduction of fumarate B. the oxidation of succinate C. the reduction of oxaloacetate D. the oxidation of malate E. the formation of α-ketoglutarate 3) Which member of the electron transport chain is also an enzyme in the TCA cycle? A. ubiquinone: cytochrome C oxidoreductase B. alpha-ketogluarate dehydrogenase C. NADH dehydrogenase D. Succinate dehydrogenase E. Cytochrome C oxidase 4) In anaerobic muscle, exercise will cause which one of the following to occur? A. lactate levels will increase B. ethanol will accumulate C. pyruvate conversion of acetyl-CoA will accelerate D. glycolysis will stop E. none of the answers is correct Biochemistry 405 Exam 1 VERSION B October 23, 2015 5) Which one of the following compounds is at higher level of oxidation (more oxidized) than CH3CHO? A. CH2=CH2 B. CH3CO2H C. CH3CH2OH D. CH3CH3 E. None of the compounds above is correct 6) Which type of reaction occurs more than once in glycolysis? A. aldol cleavage B. oxidative decarboxylation C. condensation D. phosphorylation E. dehydration 7) Consider the following metabolic reaction Succinyl-CoA + Acetoacetate  Acetoacetyl-CoA + Succinate G = -1.2 kJ/mol The G for the hydrolysis of Succinyl-CoA is -33.9 kJ/mol. What one of the following is the G for the hydrolysis of Acetoacetyl-CoA in the following reaction? Acetoacetyl-Coa  Acetoacetate + CoA A. +32.7 kJ/mol B. +35.1 kJ/mol C. -35.1 kJ/mol D. -32.7 kJ/mol E. none of the answers is correct 8) Which one of the following citric acid cycle enzymes produces GTP? A. aconitase B. isocitrate dehydrogenase C. succinyl-CoA synthetase D. fumarase E. succinate dehydrogenase 9) The first half of glycolysis includes which one of the following steps? Biochemistry 405 Exam 1 VERSION B October 23, 2015 A. ATP synthesis at the substrate level B. The incorporation of Pi into a triose phosphate C. The reduction of NAD+ to NADH D. The formation of 1,3-biphosphoglycerate E. Formation of fructose-1,6-biphosphate 10) Which one of the enzymes listed below consumes ATP during glycolysis? A. pyruvate kinase B. phosphoglycerate kinase C. glyceraldehyde 3-phosphate dehydrogenase D. enolase E. phosphofructokinase 11) The following is a table of E values (in Volts) for an electron transport sequence of a hypothetical organism. The predicted sequence of the carriers for electron transport is: 1 NAD+ NADH 2 -0.32 2 FProtein b(red) 2 -0.62 3 Cyto Cyto3+ 1 0.22 4 Ferro Ferro 2 0.85 5 Flavo Flavo(a) 2 0.77 A. 2 1 3 5 4 B. 1 2 3 4 5 C. 4 5 3 1 2 D. 4 5 3 2 1 E. 1 2 3 5 4 12) Which one of the following polysaccharides cannot be digested by humans? A. amylopectin B. amylose C. cellulose D. glycogen E. starch Biochemistry 405 Exam 1 VERSION B October 23, 2015 13) Which complex of the electron transport chain is unable to generate sufficient free energy to translocate protons across the inner mitochondrial membrane? A. Complex I B. Complex II C. Complex III D. Complex IV E. All the complexes are able to generate sufficient free energy 14) Which one of the following is the number of isomers that exist for an aldose hexose monosaccharide? A. 14 B. 10 C. 16 D. 15 E. 18 15) Which one of the following is a cofactor used in the decarboxylation reaction of alpha-keto acids such as pyruvate? A. ADP B. FAD C. TPP D. Coenzyme A E. NAD+ 16) Which one of the following is a cofactor used in the decarboxylation reaction of alpha-keto acids such as pyruvate? A. ADP B. FAD C. TPP D. Coenzyme A E. NAD+ 17) Which one of the following enzymes is known to be regulated by phosphorylation? Biochemistry 405 Exam 1 VERSION B October 23, 2015 A. enolase B. hexokinase C. malate dehydrogenase D. citrate synthase E. pryuvate dehydrogenase 18) The isomerization of citrate to isocitrate: A. is a major regulatory step for the citric acid cycle B. is an oxidation reaction C. is the only unnecessary step of the citric acid cycle D. protects cells from the toxic effects of arsenite ion E. converts a tertiary alcohol, which cannot easily be oxidized, to a secondary alcohol that can be oxidized 19) Which one of the following enzymes in glycolysis is inhibited by citrate? A. hexokinase B. pyruvate kinase C. enolase D. phosphofructokinase E. glyceraldehyde-3-phosphate dehydrogenase 20) One reason why arsenic is poisonous is that arsenite ion (AsO3) reacts with reduced lipoamide to form a stable complex. This derivative of lipoamide cannot be reoxidized. Which one answer below lists the metabolic concersion(s) that cannot occur in the presence of arsenite? A. ketoglutarate  succinyl-CoA + CO2 B. isocitrate  ketoglutarate + CO2 C. pyruvate  acetyl-CoA + CO2 D. pyruvate + CO2  acetaldehyde + CO2 E. A and C 21) Which one of the answers below lists the arrow(s) pointing toward a “high energy” phosphoanhydride bond? Biochemistry 405 Exam 1 VERSION B October 23, 2015 A. 3 B. 4 C. 4 and 5 D. 2 E. 5 22) For each acetyl CoA entering the citric acid cycle, two carbon dioxide molecules are formed together with: A. 2 NADH and 1 FADH2 and 1 GTP B. 2 NADH and 1 FADH2 and 2 GTP C. 3 NADH and 3 FADH2 and 1 GTP D. 3 NADH and 2 FADH2 and 1 GTP E. 3 NADH and 2 FADH2 and 1 GTP 23) Consider the following two half reactions: Acetaldehyde + 2H + 2e- <__> ethanol E = -0.197V NAD+ + 2e + H <__> NADH E = -0.32 V What is the E for the combined reaction? A. -0.123 V B. +0.123 V C. +0.517 V D. -0.517 V E. none of the answers is correct 24) Which ONE of the following is the water soluble electron carrier in the thylakoid lumen that donates electrons to oxidized P700 in Photosystem I? A. O2 B. Plastoquinone C. Protons D. Antenna pigments E. Plastocyanin 25) Which ONE of the following is NOT an electron carrier in the respiratory chain of mitochondria in animal cells? Biochemistry 405 Exam 1 VERSION B October 23, 2015 A. FMNH2 B. Ubiquinol C. Reduced cytochrome c. D. Reduced plasoquinone E. A reduced iron-sulfur protein 26) Which ONE of the statements about respiratory complexes (a-d) is INCORRECT? Or are all of the statements (a-d) correct (e)? A. complex I oxidizes NADH and it reduces ubiquinone B. complex II oxidizes FADH2 and it reduces ubiquinone C. complex III oxidizes ubiquinol and it reduces cytochrome c. D. complex IV oxidizes cytochrome c and it reduces O2 E. All of the statements (a-d) are correct


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