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# Class Note for ESRP 531 with Professor Felsot at WSU 17

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This 3 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at Washington State University taught by a professor in Fall. Since its upload, it has received 17 views.

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Date Created: 02/06/15
WASIHNGTON Slim Instructor Allan Felsot UNIVERSITY afelsottricitywsuedu 50 Response median Numbers Responding Lecture 6 DoseResponse Relationships Fall 2005 Dose mgkg 100 Mor all Population Response y Plotquot 2 Cumulative 10 8 72 g S 20 4 16 3 gt 50 so 4 48 5 40 4 75 o g E o 50 5 00 o E 60 5 25 7O 5 52 0 80 5 84 Log Dose Dose mgkg 90 628 Adverse Response M l ll quotHill I 95 FL 100 i i Analogous to the 95 confidence interval or limits calculated for univariate statistics i an experiment was conducted 100 times then the CL or FL represents the distribution of the data or interval of data that is predicted to capture the mean CL or median FL response or any measured variable 95 times Thus there is a 5 chance that the mean or median response was not captured by the 0 estimated interval Dose Lo Dose For any two measured populations it the g estimated 95 FL tor the response overlap then we cannot conclude there is a difference between Note that the FL are narrower about the median response them With less than 5 PrObability 0f Type error Probit Tr 50 7 Important Information Contained in the A Slope of the DoseResponse Curve Median Hpcpons i Represents variability in population response t Characterizes margin of safety Response of different species exhibiting same median response to same chemical The slope is the key to understanding the variation in population response and it can be used to compare two or more populations or alternatively how different factors affect the response of one population CumUIatiVe Response Numbers Responding Log Dost ie Var at on n response of two d fferent spec es react ng to the same chem ca in Var at on n response of 5 ng e spec es react ng to two d fferent chem ca 5 Numbers Responding W l 7 Dosc Dosage Concentration Dose Dosage concemratlon L Response 50 50 Log Dose Lng Dnse Cumulative Response 2002 Experiments t39f lt 3k Example 1 Testing the response of neonate codling moth larvae to different concentrations of insecticides on leaves Trea leaf disks wl h differen concen ra ions of lnsec lclde solu ion Expose 5 larvae per leaf in mul lple replica e con alners Wal specified lme period e g 3 h or 24 h and coun 0 al larvae and dead larvae Transform da a o morally Correc for con rol un rea ed leaves mor all y if necessary Momsquot ly 0 l 001 l 0 U 1 1 3910 Cancnnnannn rm Lml Sumlcv m nml 100 ugcmz 90 LC50 LC95 80 Assail 0007 0166 Guthion 0019 0095 Mortality kGuthion 10 G l l l l 1 000001 00001 0001 001 01 1 Concentration on Leaves pgcmZ 2003 Experiment in Somet mes we want to know how ong t takes or an organ sm to respond For example this might be an advantage in pest control it Take treated eaves or other env ronmenta samp es and ho d arvae on them for spec fied per ods of t me fconduct experiment in the lab you can still use matrices with different concentrations just vary the time to observation of response at enough intervals to be able to validly estimate a time response function Calculated Pvgbn Rimka 952quot Lower Flmzoal lell 9 0 l p 9501 Upper Flducxai LIIHII Z 397 00 E 50 3 40 e a 30 Observed Data 1 V 10 0 7 100 50 0 50 100 150 200 250 Time minutes I Ll t 71 min 1 52 all Recovery of Assail residues and significance relative to dose response function for neonate codling moths on leaves Type of Orchard Sprayer p333 Assail Airblast 9 Proptec 015 Mid Canopy TOP Canopy 0102030 4050 0102030405060 Days After First Cover Spray i How long would it take codling moth larvae to die if exposed to apple leaves directly sprayed or apple leaves from the opposite side of the tree that were not directly sprayed W Orvsewed Modality I swayed Me of tree 0 Unzp ayud sud of tree Mortality 2 LED 71 mml an 10 L150 54 mm 45 631 0 l V 400 100 150 200 Time mlnules

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