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# 350 Note for BIOL 13500 at Purdue

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This 20 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at Purdue University taught by a professor in Fall. Since its upload, it has received 24 views.

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Date Created: 02/06/15

BIOL 135 LAB PRACTICAL LIST Calibrate and Use Electronic Balance Calibrate and Use Beamed Balance Calibrate and Use Pipetmann Calibrate and Use pH Meter Calibrate and Use Spec 20 Prepare Microscope for Kohler Illumination Use Sterile Technique to Obtain Liquid Culture Streaking for Single Colony Isolation Calibrate Eyepiece Graticule With A Stage Micrometer BIOL 135 Written Final 1 What are the SI pre xes abbreviation values Example p 10quot12 2 Briefly describe what we mean by independent and dependent variables 3 What do each of the symbols mean 4 What does each color and number represent on a diamond label 5 What do you put on a label of each container of a substance 6 How do you properly dispose of gt1 mL of liquid biohazard waste if you can t send this sample to our department s Media Prep Service to be autoclaved 7 Convert 945 X 10quot7 nmquot2 8 Convert 0031 X 10quot5 nmoIL to umoIL 9 How many cmquot3 are there in 057 uL of water expressed in scientific notation 10 Four plots of land each 356sq feet were planted with a variety of wheat The plot yields were as follows 351 306 369 298 Calculate the range mean and standard deviation 11 In your own words distinguish between an arithmetic mean and a geometric mean 12 As part of a study on plant growth a plant physiologist grew 13 indvidiually plotted soybean seedlings ofthe sam strain She raised the plants in identical conditions and measure the total sem length cm for each plant on day 0 and 16 days later Stem Growth 202 220 197 229 221 215 233 220 209 200 219 194 215 Find the mean standard deviation and standard error 13 What is the max capacity you can use with electronic balances 14 What do we call statistical methods which depend on data sampled from a population with an underlying normal distribution or whose distribution can be made normal by mathematical transformation 15 What is the null hypothesis and alternative hypothesis 16 Do we use a ttest or Xquot2 test with qualitative discrete variables 17 What is the key code word or phrase for using the multiplication rule of probability 18 Suppose there are 5 different history courses 3 different english and 4 diff bio A Ofthese you need to pick 1 hist 1 eng and 1 bio How many ways can you do this b What if you only need to pick one ofall these courses How many ways can you do this 19 What is an allele 20 What are the genotype and phenotype of offspring with the parental cross of a homozygous wild type ebony ee y with a homozygous mutant ebony ee individual 21 What are the genotypes and phenotypes as well as the expected ration of the phenotypes of a cross ofthe offspring of the previous question 22 What are the genotypes and phenotypes as well as the expected frequencies of the phenotypes of a cross of the different categories of offspring in the prior question with a homozygous mutant ebony fly 23 Know how to perform a ttest 24 Draw a cell in in anaphase ofmitosis anaphase l of meiosis and anaphase ll of meiosis stating what is happening It has two pairs of chromosomes 2n4 25 How would you go about doing three sevenfold serial dilutions of a solution with water tubes such that the total volume in the last tube is 700mL What is the dilution ratio in each tube Draw and label a diagram 26 How would you go about making a 120 uL ofa 15vv concentration of colored water and clear water 27 What size Pipetman P2102010020010005000 would you use to make a single delivery ofa 110 uL of solution 28 How would you go about making 1 L ofa 05 M solution of NaH2PO4 H2O MW119976 FW13799 with R0 water 29 You have a 5X stock solution of01 M NaCl What is the concentrationmolarity of the 5X stock solution of NaCl Use 585 g as the molecular weight of NaCl 30 Pure water at 250 contains how many molL of H ions and how many molL of OH ions and what is the ion product of water Kw 31 What is the de nition of an acid and a base according to Bronstead Lowry concept of acids and bases and what is the mathematical description of pH 32 What is the OH of a solution you measure as having a pH of765 33 lfa weak acid has a Ka of 38 X 10quot4 then what is the H ofa 004 M solution of this weak acid 34 lfa weak base has a pr of 748 then what is the H ofa 001 M solution of this weak base 35 You need to make a serial 15 dilution in three tubes and the nal volume of the third tube needs to be 46 mL What volume will you take from the second tube and put in the third tube ofdiluent 36 How many nmquot3 are there in 969889 pmquot3 37 How do you calculate the pKa from Ka of a weak acid 38 A 03M weak acid solution is known to be 95 ionized Find this solution s H OH and pH 39 How would you prepare 500 mL of an NaOH solution have a pH115 Use 40 as w 40 Calculate the pH of a buffer that has 0085 M sodium acetate and 0015 M of acetic acid The pKa of acetic acid is 4756 41 When building your standard curve of known concentration what are your dependent variables and what are your independent variables 42 As you known a spec s record displays both A and T What is A in the following a T56 b T16 43 The BeerLambert law can be expressed as AEdc What is the absorabnce of a solution of 01 mM of pnitropheno if the extinction coefficient of pnitrophenol is 18 X 10quot4 M1cm1 and the length of the light path through the solution is 10mm 44 You dissolve 126 g NaOH in water to make 5440 mL of solution Use 40 for mw What is the molarity of this solution What is the wv of this solution 45 Sea water contains roughly 280 g of NaCl per liter MW5844 What is the molarity of NaCl in sea water What is the NaCl wv of sea water 46 127 g of NaCl and 199 g of NaN3 are dissolved in water to make a 55 mL solution MW for NaCl5844 MW for NaN36501 What is the molarity ofthe NaN3 in the solution 47 The Trinder s assay is an assay for what substance 48 What color does the Trinder s reagent become in the peresnceof the substance the Trinder s assay is used 49 Two concepts very important to keep in mind when doing an assay like the Trinder s assay where we use a colorforming reagent One is that the data used to produce the Standard curve must show a linear relationship between absorabnce and concention ofthe compound What is the other important concept 50 The levels of a substance in the plasma of a healthy human flucates between 70 140 mgdL Forthis problem use the average of 105mgdL How many liters of human plasma do you need for 07kg of this substance 51 You use a spec to build a standard curve ofdifferent concentrations of CoCl2 Conc CoC2 1 10 20 30 40 Absorabnce 002 018 038 058 073 a Plot these points b Do a Linear Regression c What valuses does rquot2 for between What does this tell you about the data d What is the concentration of CoCl2 with an absorbance of 040 52 All amino acids contain what elements and a couple amino acids also contain what element 53 Describe in your own words 4 of the guidelines for sterile technique 54 Describe in your own words how to adjust a Bunsen burner for a hot blue flame 55 What principle is the technique of streaking for single colony isolation based on 56 If you place an object 23 cm from a biconvex converging lens with a focal length of 15cm what is the distance of the image produced from the lens 57 If you place a 5cm high object 24 cm in front of a biconvex converging lens with a focal length of 15 cm a0 what is the distance ofthe image from the lens and b what is the height of the image produced 58 When we say BSA has 607 residues what do we mean by residues 59 How many different amino acids de we find in proteins or polypeptides of organisms 60 A dalton is equal to what 61 When building a standard curve to measure the conc ofa substance in a solution you need to make a duplicate series of concentrations What do you always do when you make your duplicates 62 You need to make a fivefold serial dilution in five tubes You will start with 1 mL of your starting solution and you will use DI as your diluent What is the dilution ratio of each of your five tubes What is the nal volume of your fifth tube 63 As you know glucose levels in the plasma ofa healthy human fluctuate between 70140 mgdL For this prob use the average of 105 mgdL What volume ofhuman plasma do you need for 14 kg of glucose 64 In terms of their source of carbon as a major nutrient most bacteria are characterized as being what 65 What is the term we use to describe rodshaped bacteria 66 What does morphologically indistinguishable mean 67 What is the different between the peptidoglycan layer of a gram positive and a gram negative bacteria 68 If you are counting the density of cells using a hemocytometer and you count 53 cells in the center 25 squares but this count is after a 15 dilution of the original sample of cells how many cells mL1 do you report forthe original sample of cells 69 lfthe diameter ofa spherical cell is 24um then what is this cell s surface areato volume ratio 70 Write out the general exponential functionequation and indicate what terms represent variables and what terms represent constants 71 What do each of the letters in the law of growth equation represent NtNO equotkt 72What are the four general steps in order for preparing tissue for exmination as histological sections 73 What are the three kinds of muscle tissue in vertebrates 74 What is the difference between connective tissue and epithelial tissue 75 Nervous tissue consists of two general type of cells What are they 76 Sketch what a section through each plane would look like and label them Histological Sectioning 77 Perform a Chi Test

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