Class Note for MCB 422 at UA
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This 4 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at University of Arizona taught by a professor in Fall. Since its upload, it has received 21 views.
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Date Created: 02/06/15
102908 Sex in yeast Mating combines haploid genomes to diploid genomes us39 female amp male produce egg amp sperm yeast mating types a and alpha produce spores dauer state that produce haploid cells active state our human haploids are shortlived gamets but yeast haploids are free living organisms that are virtually identical to diploids 102908 2n 2n some strains can proliferate as diploid cells 2n meiosis and sporulation usually immediately after conjugation Spores hatch conjugation triggered by starvation I proliferation of haploid cells yeast exists as diploids haploids and haploid spores Q the two sexes of haploids a and alpha are Virtually indistinguishable except a cells produce a pheromone that is highly attractive for alpha cells alpha cells produce a pheromone that is highly attractive for a cells upon pheromone detection cells stop cell cycle mitosis etc and get ready for mating technically they schmoo toward each other upon touching cells and nuclei start fusing 102908 102908 Life c 016 of east Mitosis Mating amp Conjugation o x hard tim es Teens schm 00 hapl 01d Ascus Wlth Meiosis amp 4 spores Sporulation 102908 diploid Mating amp Conjugation Ascus with Sporulation 4 spores o 1 rLi 31 1139 L 77 Sexy Yeast aspecific genes ON by default alphaspeci c genes Off by default a1 gene 11 haploidspeci c genes a CC gt ON by default aspeci c genes gt OFF 31131132 alphaspecific genes ON alphalH haploidspecific genes ON by default alpha cell 1029 08 aspeci c genes aspeci c genes I gt OFF alpha speci crlebsy default 3Eal haz alphzieci c genes ON 31 gene OFF by defamt alphal haploidspeci c genes haploid ecific genes 3 0611 ON by default alpha cell gt ON aspeci c genes lEha2l default on OFF gt alphaspecific genes gt OFF a1 gene default off haploidspeci c genes OFF default on 102908 cell 102908 102908 Topic of Path nder Phases of the Cell Cycle M PHASE mitosis l nuclear 39 onl cytokinesis cytoplasmic 5 PHASE lDNA replicationl 1 02908 Behavior of temperaturesensitive cell cycle control mutants example mutation in Gl A PERMISSIVELOWJTEMPERATURE Bl RESTRICTIVEHIGHlTEMPERATURE uselive m 61 Ga 61 gt Q a 39 At restrictive temperature cell s can t divide At permlsswe temperature 06quot dIVIde normal since the cell cycle will be arrested at normal and are found In all phases ofthe the mutated phase CYCIS Consequently all cells are found in the same 102908 phase ofthe cycle
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