Class Note for MCB 411 at UA
Popular in Course
Popular in Department
This 35 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at University of Arizona taught by a professor in Fall. Since its upload, it has received 15 views.
Reviews for Class Note for MCB 411 at UA
Report this Material
What is Karma?
Karma is the currency of StudySoup.
You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!
Date Created: 02/06/15
August 29 2005 oAnnouncements oLast two slides from Thursday Know your molecules review oReview of paper oThe last 6 most important things you should know about DNA oHomework due Thursday Why only ATGC base pairs malor groove may groove 2 H H Y N QllllllIN N rHllllll 0 N NgtHHHmNC H N ANlllllllHrNgtrT 1 N I N NHll lll N463 0 V H quotinor grocNe 39eror growquot5 Figure 7 7 parl1 of 2 Molecular Biology ofthe Cell Am Edlllon Why only ATGC base pairs ma pr groove main groove H H VN OHHHHN N HHHLD N ZfG gNkwa c N ANMHH N T N C N N gtF N f Nme V 0 V y H minor growe Wnor growquota Figure 7 7 part 1 of 2 Molecular Biology onhe Cell am Eamon Hydrogen bonding size Strength of a single bond is not everything One human Chromosome on average is 130 million base pairs in length So that is X 25 assuming average GC is 50 325 million hydrogen bonds plus hydrophobic interactions 2 key concepts so far Molecules interact primarily on the basis of hydrogen bonds salt bridges and water freeing hydrophobic interactions Bases pair because of chemical complementarity size shape and number of hydrogen bonds between bases failure of bases to pair with nonpartners is a critical component of information in DNA know your molecules review Identify bases sugars phosphates nucleotides as such not draw them Know amino acids and properties of side chains Sketch a DNA molecule using symbols for different parts Know linkages to numbered carbons of ribose Contributions by James Watson BREAKDOWN OF MESSENGER RNA DURING IN VITRO AMINO ACID INCORPORATION INTO PROTEINS Tissi res A Watson JD Proc Natl Acad Sci U S A 1962 MESSENGERRNA ATTACHMENT TO ACTIVE RIBOSOMES Risebrough RW Tissi res A Watson JD SOME RELATIONS BETWEEN DNA AND RNA Rich A Watson JD Proc Natl Acad Sci U S A 1954 RICH A WATSON JDPhysical studies on ribonucleic acid Nature 1954 May 22 1988Associate Director of Human genome project 1989 Director Contributions of Francis Crick CODES WITHOUT COMMAS Crick FH Griffith JS Orgel LE Proc Natl Acad Sci U S A 1957 Crick FH Codonanticodon pairing the wobble hypothesis J Mol Biol 1966 Moves to Salk Institute San Diego Linking numbers and nucleosomes Crick FH Proc Natl Acad Sci U S A 1976 Long axons within the striate cortex their distribution orientation and patterns of connection Mitchison G Crick F Proc Natl Acad Sci U S A 1982 Crick F Koch C Kreiman G Fried Consciousness and neurosurgery Neurosurgery 2004 1 Nucleotides Phosphate pentose base cyclic nitrogen containing compound 2 Chargaff s rules 3 Alpha helix for proteins Pauling s triple helix Side view of Bform DNA Minor groove M ajo r groove Tautomers isomeric structures in equilibrium 0 O H l OkN H 0 H I I R R Thymine Thymine etc or lactam form enol or lactim form i H i O O H N N N N r H lt A r I H 2N N III H2N N If R R 5 r Guanine Guanine f keto or lactam form enol or lactim form The contribution of Rosalind Franklin Died of cancer at age 37 quotThe picture that Wilkins showed Watson was Rosalind Franklin s The xray diffraction photograph of the paracrystalline or B form of DNA that Franklin took over the weekend of 1 May 1952 and later pub lished in her paper with Gosling that accompanied Watson and Crick s in Nature 25 April 1953 Photograph courtrsy Maurice Wilkins w 32 How Q92 92 Aform Bform Z form WC 75 H20 95 H20 poldedC What kind of bonds link the three parts together What kind of bonds link the 2 strands together Covalent bonds within strand Hydrogen bonds between strands What does this imply DNA is very stable and efficient Stability is good for your own cells some of which are as old as you are This is useful for forensics or studying fossils This is useful for working with DNA 025 of a mammalian cell is made up of DNA 8a DNA is a dynamic molecule When you heat DNA strands denature Which base pairs melt first replicalion origin II mmlmmllllllllllllmmlmmllmmmllmnmnmnnn WW LOCAL OPENING OF DNA HELIX WWW WWW FINA PRIMER I SYNTHESIS W Wm Hmymmuuwv NEW DNA CHAIN STARTS LEADINGV STRAND SYNTHESIS W 4 7 A FINA PRIMERS START ADDITIONAL NEW DNA CHAINS lagging strand leading strand of forkI offorkz mg mm W m unuqu I wyuy leading strand Iagging strand of fork 1 0f fork 2 FLORK 1 FDRK2 gt Figure 5729 Molecular BIoIogy of the Ce 4th Edition 8b When you boil DNA strands melt When you cool it quickly single strands reanneal to themselves or to other strands 8b When you boil DNA and cool it slowly complementary strands find each other This is the basis of PCR Southern analysis DNA sequencing In situ hybridization Microarray analysis The two strand of DNA are different Watson Crick origin of replication A 439 A Escherichia coli K42 4639221 nucleotide pairs terminus of replication Figure 1730 Molecular Biology of the Cell 4th Edition The two strand of DNA are different Watson Crick The two strand of DNA are different Watson Crick palindromes 10 DNA has 2 faces A major groove and a minor groove minor groove major groove Figure 776 Molecular Binlogy of the Cell 4th Edition mayor groove Jma39xor grameh Fl f K Y r L r merN vquot m Hum V G N HmvaC H 5 N ANumuH N T N y i x NHMM 39H we 1 I quot7mm growa quot39Hnor groo Figure 7 7 part 1 of 2 Molecular Biology of the Cell 11th Edi on maxor groovek mayor groove NiHWI 1 7 nun M Q 3 C NIUHHFFN G N I TN7HHHWNA N 7 2 N quot iggllfsrwth NE w 4 RY g 3 H 1 minor groo lequot Figure 777 pan 2 of 2 Molecuiar Biology of the Cell Am Edition major groove HODC Figure 74 minor groove gt040 ngtcgt MoIecular Bimogy loe Cell 4th Edition gto n KEV Hrbond acceptor 0 Hbond donor 0 hydrogen atom O methyl group 3 5 TTK Finger 1 TTK Finger 2 Figure 7 28 part 1 of 3 Molecular Biology of the Cell 4th Edition 11 The Watson Crick model provided a hint as to how DNA replicates 12 The model for DNA proposed by Watson and Crick fits well with what as being discovered about genetics and cells at the time Crick s key functions of Biology This is the subject of the rest of MCB411
Are you sure you want to buy this material for
You're already Subscribed!
Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'