Review Sheet for ECOL 320 at UA
Review Sheet for ECOL 320 at UA
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This 10 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at University of Arizona taught by a professor in Fall. Since its upload, it has received 12 views.
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Date Created: 02/06/15
What is the major functional di erence of Theta vs Rolling Circle replication speci cally in regards to the tandem linear repeats found in the R C replication You won t be responsible for this Rolling circle replication may not stop after making one complete genome This happens in some bacteriophage in making extrachromosomal copies of the nuclear rRNA genes in some eukaryotes and possibly in some chloroplasts and mitochondria It can be used to amplify a genome for various purposes It has some interesting genetic consequences CBA CBADCBADCBA Do we need to know all of the information about the lac operon ie lac Y does and the lac operon mutants Yes You need to understand the function of lacI P 0 Z and Y well enough to infer the phenotype of a haploid or diploid cells from their genotypes With respect to the various lac mutants described in the text Do we need to know the information about the scientistsresearchers and who found what Not for this exam However in the next set of lectures I will make a timeline of major discoveries and add names and dates to it as we proceed You will need to know those Of the names we have encountered thus far Mendel and Watson amp Crick will be on it Can you explain conserved regions again A conserved sequence or region is one that varies only a little between species and even less within species They are usually functionally important sequences We will discuss this in detail and explain why in the section on population and evolutionary genetics What would you like us to know about the Wobble mechanism Only that some bases in the anticodon of tRNAs can form H bonds with more than one base in the third codon position of mRNA and this is one of the reasons why the code is degenerate 39 Do we need to know what happens during meiosis in an animal Like that spermatagonia Change into something else Yes you need to know the names of the cells at each stage of meiosis in both male and female animals 0n the sample exam question 6 states the Methionine is the N terminal amino acid in globin My question is Methionine the N terminal amino acid in everything or just globin Yes although in some cases a protein may undergo processing that removes some of the N terminal amino acids The N terminal Met might be modified in some plants so it becomes one of the odd amino acids other than the 20n in the code I m not sure Remember that ATG AUG is always the start codon Also in the notes you stated that Polypeptide is made N terminal to Cterminalbut I cant see that very clear on the diagramcan you go over it a little to make sure I have it right See text p 400 401 p 4 R1 is CHZCHZSCH3 quotiiigg ti quot How do I know this 1 n I I la y 4 L r 1 i H TCL DHH ITI 1C DH H 391 2 cm WWW Hng 1013 H R H R H R R KB npum 1mm I 39V1ikk g mv m H H H H 0 Amino group Carboxyl H77N i w rOU l l l l l H H 0 g p H R R2 R3 Ra I ll 2 3 a H N C C OH Atrium Larlm u m R on carbon Side chain Simple Model of lac System Genotype Nature lacZ 39 Null mutation in lacZ lacY 39 Null mutation in lacY lac0 c lac0 can t bind repressor constitutive always on lacP 39 Promoter can t bind RNA polymerase operon not transcribed lacI S Repressor can t bind inducer superrepressor lac PO Z Y A Repressor protein 4 0 Id 11 EtaVG r f f 4 f RNA polymerase Phenotypes of lac Operon Mutants in Diploids Synthesis of Genotype lac mRNA Lac phenotype 4 v F lacO lLMT39 lacOlncZ Constitutive 2 F luLO 1152 lacOEacZ COHSNlUlIVE A 3 F lucl lacZ r lac39lacZJr lndudble 4 F luLI39illch39 lacl lacZ39 lnducihle 4b 5 F l COLlHCZ lacO lch39 lndudble l 6 F laLOE h1inr lacoJr lacZ Constitutive 7 F lucNacZ Incl lacZ Umnduclble A 8 F lacl acZ39 ladsac Unmducwble 9 F lacP39 lacZ lacP lucz lnducible VD F ladquot lKMZ r tchquotlz1rZ lnduclble H F lacP lacZ IMP39 luLZ Unmduclble 7 12 F lacFquotr lacZ r lacF IBCZ39 lnduclble J acob Monod and collaborators deduced how the lac operon is controlled from these data and from the map position of the mutants Note 1000 and IacP mutants only affect expression of lot gnes on the same chromosome while lac mutants can operate at a distance from another chromosome Tryptophan Auxotrophs Chorismic acid ASase 4 trpE AnthraniW PRTase 4 trpD PEA CDRP InGPSase 4 trpC InGP TSaseB 4 11773 Indole TSaseA 4 mm L Tryptophan G rows On CM MM MMTrp MMindoIe MMInGP MMCDRP trp trpA trpB trpC trpD trpE For some reason question 1 of quiz 2 is confusing me 1 2 Below is a eukaryotic DNA molecule caught in the act of replicating Note that there are discontinuous and continuous strands Letters a d refer to ends of the molecule State Whether each of the following is a 5 or a 3 end a 3 c 5 b 5 d 3 arm r39 Question 125 from text A synthetic RNA molecule has the sequence 5 CGUUACCACAUGUCGCGAACUCG How many reading frames are possible if this molecule is translated in vilTo In vivo The in vilro system does not require an AUG start so it can start anyWhere Thus there are three possible reading frames In vivo translation requires an AUG start so it starts With the first AUG Which uniquely determines the reading frame
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