Class Note for OPTI 696D at UA
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Date Created: 02/06/15
Fluorescence Spectral Characteristics of Fiber Optic Imaging Bundles Josh Udovich November 30 2005 Outline IWhy fiber bundles I Current uses of fiber optic imaging bundles I Autofluorescence of these bundles really is an issue I Testing setup I Fiber fluorescence I Conclusions Why Coherent Fiber Bundles 1 Point to point image relays Flexible Variable lengths 1 Used for remote imaging applications Uses of Imaging Bundles I Endoscopy I Nonrigid image guides I Two photon microscopy I Confocal microscopy Limitations of Coherent Bundles I Resolution of fibers limited by ber size and spacing I Crosstalk between fibers I Losses high outside of visible region I Autofluorescence from fibers can obscure optical signals Fluorescence Spectrum of Syto 16 citation E 9 w m 4 O x D a o i Ci 0 a 2 O 3 LI Fluorescence emission 400 450 500 550 600 650 700 Wavelength nm J Excitation peak is at 488 nm 1 Emission peak is at 518 nm Multispectral Images 3 w s Q Q N Q a b 39R quot 39 f 39 g g h i 939 539 0 e I s3 7 0 O o c on IR Autofluorescence pf Fiber Cores O O i 39 0 o l Top Image Q 39 g O 488 nm eXCItation I t 39 a c 39 515 nm emission D 30 nm bandpass o I Bottom image o O 488 nm excitation 515 nm Iongpass emission filter to dichroic cut off Fibers Used I Sumitomo IGN0830 I Both bundles have I Fujikura FIGH30 30k imaging fibers 850N 2 tun diameter 3 tun center to center spacing l 720 um active area l 1 mm in total diameter I Germanium doped silica cores Measurement System 1 Double grating excitation emission fluorometerwith PMT J Delivered through a N quartz Test Fiber 1 Variable parameters Excitation and Emission Wavelength Excitation Bandwidth Emission Bandwidth Integration Time Fiber Fluo escence EEM Sumitomo Fiber Fluorescence FUQeUUB 0539DDB 12919UDE 8359UDB 5119UDS 19558005 lll A E C v C O Iquot M F U X J 1949DUS 259DD4 i5149 D4 m8596 4 10009000 Emissionnm Sumitomo Line Scan at 488 nm 1nm steps in emission detection 1 nm excitation and emission bandwidths Fiber Fluorescence EEM Sumilomo Fiber Fluorescence Fujikura Fiber Fluorescence 70925000 751mm 7 053mm 272mm 7 29mm 743mm J i l BSEMZIEIE 9549 A g 37724005 E 7 anwa05 5 5 c S 2 90024005 2 9502005 2 E U EBBEMUS m 10420005 I 53420305 270mm l 03520005 EMEWUA 573mm 7 0592mm r032003 h 39 000 000 600 a 300 400 500 600 700 800 EmISSIONmM Emlsslonmm SHED 15000 l l l l l l ZEUEI ZUUU r 10000 7 3 E g l g 0 3 E E E E 1000 7 7 5000 r EDD n l l l l l u l l l 405 500 505 10 515 520 525 530 m 695 500 505 5m 515 520 525 Emlsemn Mn Emlsslun run Fujikura Fiber Fluorescence Spectra Fujikura Fiber Fluorescence A E N r o z m E U x IIJ magmas T Emissionnm Raman Scattering D V 1 1 C incident scattered D l z 2 1 l I For 488 nm incident and 499 nm scattered peak Conclusions I In UV there is significant autofluorescence due to material properties of the fibers I Throughout visible region fiber auto fluorescence is close to excitation wavelength IAppropriate filter and dye selection may be able to reduce fiber autofluorescence due to Raman scattering Acknowledgements I Ned Kirkpatrick Urs Utzinger Art Gmitro I Andrew Rouse
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