Class Note for MIC 205A at UA
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This 10 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at University of Arizona taught by a professor in Fall. Since its upload, it has received 15 views.
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Date Created: 02/06/15
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already mequot durlng mspeallon and addnlcnal le l mal lurlhnrdasalba anddlllemnllaln the unbeawumvlishm ll Imliul 31m ylched spam mlmmalmn an mummy mumhulngy mm column an Sam media ms Process kwwn ussubclllll lw Imclvalnkmgatiny nu olgmwlh m an imam mluny m lmculalmg 3 54pm medlum mg ism way in mm a pun cullms thatcnnuim anlya singln species a mlcmha mlcmbex SDeclnlizsalem lncludehmhemlml lasls la delermme melnbnlx ulwille swllic lo the was Immunnl aiclm nm weliclnalyvs Mammal Eel m mm EnnImmn 4 rain w uninV il Mcmxm w ovwn quotmm in quotml ccla u mots warm vmblo M smug cox m MNIHYJ mum m an anthem niw Why might there be more than one microbe in the sample Streak Plate Note This method only works ii the spreadlng tool usually an inoculating loop IS reslerili zed aner each oi sieps 174 1 l Q m tamp f 1 V 2 3 4 a Steps in a Slreak lee l Appearance of Streak Plate After Culture Loop Dilution in sun in L500 niiunun Spread Plate mamy sllch39 1 ImSulenlSprIld m Chemical Content Synthetic all components known and quanti ed Nonsynthetic Complex some components not known or quanti ed Functional Types of Media Enriched Selective Differential Microbial Growth Incubate at correct temperature and atmosphere Inspect to verify pure culture Identify microscopic appearance biochemical tests genetics Maintenance and disposal me m tttt an Parts of the W Light l Microscope an quot 1mm naln at light tummy mle Resolution The ability ofthe lens to distinguish two points a speci ed distance apart The shorter the wavelength the greater the resolution Best resolution of light microscopes is 02 pm 2000X Refractive Index 0 Measure of lightbending ability of the medium 0 Changing the refractive index of the specimen relative to the medium increases the contrast between the two 0 Staining is used to change the refractive index Immersion Oil 0 Air has a different refractive index than glass 0 Most light would be lost if it had to travel through air between the specimen and the objective lens 0 Immersion oil has about the same refractive index as glass and increases the resolving power of the lens Unvelvacted all Immersion ligm objectlvs lens Wlmoul immerslon oll mos Iigh is retracted lmmevsmn oll lenses Condenser Iris diaphragm Light source Bright eld Illumination Usual configuration of the light microscope 0 Light passes through the specimen and is seen in the eyepiece Staining gives increased contrast so cells can be seen Light Microscopy Cyanobacteria Light Microscopy Fungus Infected Macrophage Leishmania Light Microscopy Darkfield Illumination An opaque disk is put between the light and the specimen Only light refracted by the specimen reaches the eyepiece Emphasizes edges of structures against a dark background unstained live specimens Darkfield Treponema PhaseContrast Light comes through an annular diaphragm donut Direct rays and refracted rays are combined at the eyepiece Where rays are in phase see light Where out of phase see dark PhaseContrast Used for detailed examination of living cells View internal details Phase Contrast Macrophage Phase Contrast Phse Contrast Bacteria 9 139 S quot m r Fluorescence Microscopy 0 Fluorescence molecules absorb UV light emit visible light 0 Fluorochromes are attached to antibodies that bind specifically to organisms or cells Fluorescence Microscopy l o n ma mmwmnumm mum mm llmmnlum Immunofluorescence Fluorescence Bacteria in Paramecium Laser beam scans various depths of the specimen Electron Microscopy 0 Uses electron beam instead of light 105 shorter wave length 2 20nm Focused with magnets Transmission TEMthin section Scanning SEM 3D 0 Specimen dried stained with metals Electron Microscope Eleelron beam El Eleelron gun Electromagnetic condenser lens Electromagne l oelsmlve lam Viewing eyepiece Eleelromngnerl projeelor lens Fluorescent screen or phamguphic plate Vibrio cholerae a in intestine 39 TEM TEM Bacterial Flagella f r SEM Bio lm on Contact Dried Acnnthnmcrba Cysls Roulshapea naclcna Staining for Light Microscopy Simgle stains increase contrast 0 Differential stains differentiate between organisms or structures 0 Gram stain differentiates between two kinds of cell wall structures Gram and Cell Walls Meow l Gram Stain Gram Positive Bacteria Acid Fast Stain Gram Niegatlvefagena Mycobacteria I Spore Stain Capsule Stain 1 7 in re I quot Veggie e w a A Asmm imry What You Should Know Approximate size of eukaryotic cell bacterium and virus What limits ability of microscope to magnify specimen Advantages of brightfield darkfield phase contrast fluorescence microscopy and electron microscopy What You Should Know 0 Difference between electron microscopy and light microscopy 0 Purpose and types of information you get from Gram stain spore stain and capsule stain 10
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