Class Note for MIC 433 at UA
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This 3 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at University of Arizona taught by a professor in Fall. Since its upload, it has received 15 views.
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Date Created: 02/06/15
Afew Important Mechanisms of Viral Gene Expression DNA viruses Use nucleus to copy DNA Exception Pox Code forviral proteins to preferentially initiate DNA synthesis on the viral DNA Large DNA viruses bring their own DNA Pol etc RNA viruses Code fora RNA Pols Use cytoplasm to replicate Exception In uenza needs a nucleus Afew Important Mechanisms of Viral Gene Expression Transcription to produce mRNA mRNA in a eukaryotic cell is translated as ONE protein Viruses therefore Make mRNA coding for a polyproteins which is cleaved by a proteases or Make monocistronic mRNAs Some can use splicing machinery to make mRNAs if replication is in the nucleus Afew Important Mechanisms of Viral Gene Expression Transcription continued mRNA in a eukaryotic cell is made in the nucleus and capped at the 5 prime end m7o5 ppp5 uw 3 and polyadenylated at the 3 prime end m7G5 ppp5 NAAAAS Viruses that replicate in the cytoplasm therefore Make their own CAP or a substitute or have an IRES internal ribosomal entry site Make RNA that may or may not be polyadenylated EMC virus IRES Send Translation to produce proteins Translation is sometimes controlled by readthrough of stop codons or by reading frame changes The retrovirus example xii quotarm Splicing of mRNA by nuclear enzymes in retroviruses Translation to produce proteins Reading frame changes a slippery sequence allows the ribosome to slip and start reading in another frame The retrovirus example v hrnugl n m mm RF 1 gt1RF2 gt A Reading Frame Shi Ribosomal Frameshi ing on a retrovirus mRNA A Reading Frame Shi What causes it A RNA hairpin structure causes the slippage Translation to produce polyproteins cont d Puiypruteins are prunessed via virai pruteases re pruduce functiunai pruteins pm Praiease 3c 39Aeenwms Praiease w Prmease Prerems may aisu be prunessed by giycusyiatiun myristyiatiun
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