Class Note for CHEM 490 at UMass(1)
Class Note for CHEM 490 at UMass(1)
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This 15 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at University of Massachusetts taught by a professor in Fall. Since its upload, it has received 20 views.
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Date Created: 02/06/15
RNA Meiabolism Ch 29 Transcription amp processing Transcripfion DNA gt mRNA Similari39fies and differences vs replicaHon Promo reri consensus sequence Foofprin39iing Ini riai39ion Elongaiion Termination Processing Fri 42409 1 nucleo rides Base modifica rion TRNA rRNA mRNA processing in eukaryo res 539 cap and 339polyA Tail enhance s rabili ry Splicing ou r in rrons re rain exons Dyad symmetry O CG S WACCUGGCUCAGGACCUUCCUGAGCACACU39WV B39 RNA Figure 218 Principles of Biochemistry Ale 6 2005 Pearson Prentice Hall Inc 585 mmnn 455 Nucleotide modification Mature rRNA Figure 2943 Bipzhzmisvy Sixth Editinn moozw mama mnuunv Cleavage Methyl groups and pseudouridine d1 185 rRNA 585 rRNA III II llll 285rRNA A N N NGMethyladenyiate msA Dihydrouridylate D H C T CHZ H CH2 N o N N lt 6J1 lt NH NGlsopentenyladenyiate anSinate 16A 1 H0C O O I o HNgZLmH Hzc EkNH 4 5 5 c o NKO Pseudouridylale Uridylate 1 5oxyacetic acid ribose at C5 cmo U 2 OMethylaled nucleotide quotquot50 Nm Figure 21 25 Principles of Biochemistry Ale 9 2006 Pearson Prentice Hall Inc 5 Methylcytidylate 7Melhylguanylate lm7G 3Methylcytidylate mJC Leader E39unmmmmaua quotl I 39 quot339 Processing Anticodon Early lrunstripl Mature RNA Figure 29 2s 51mmva smn Edifian wanw HFleemnnund calw 7Methyl guanosine 7 0 539 s39rTriphosphate linkage P I a 9 Sume imas me hylaled Sometimes methylamd Cap at 5 end of eukaryotic mRNA Lipoprotein LDL receptar assembly binding Apo 3100 TTranslation 539 C All 3 Unediied mRNA RNA Editing by deamination NH 5 U A A 3 Ediled mRNA lTranslation Apn 348 Figure 29 2a Bimhzmv39sny Sith samequot mzuuzw unmanm mnp39uuy SiIe of 3 39I39riose phosphate cleavage and p isomerase gene polyadenylalion I v a 39 3r v 1 I I I I I I I I I l I I I l I I I Exon I Exon 2 Exon 3 Exon 4 Exon 5 Exon 6 Exon 7 Exan 8 Exon 9 Transcription Translated sequence messing mRNA m7GTP IIAIIIIAMWIIIIIIEBI Spliced mRIIA Figure 21293 Principles of Biochemistry4le 2005 Pearson Prentice Hall Inc Cap ponA tail 5 splice site 3 splice site Branch site i l I GUAAGU A PyNCAG j lt lntron Figure 2949 Binrhzmv39stry 5139th Edikian m 2qu w HFeeman and mum ln frame 5 stop coden 3 ll quot39 39l Guis39rogt Immune cu AG premRNA PremItIIA m GUmG mumGU AG mutation lt in an inlrnn New 5 splice site Dr 6U u Normal mature mRNA Abnormal mature mRNA Degraded Figure 35 TABLE 294 Selected human diseases attributed to defects in alternative splicing Disorder Gene or its product Acute intermittent porphyria Porphobilinogen deaminase Breast and ovarian cancer BRCA1 Cystic brosis CFTR Frontotemporal dementia 1 protein Hemophilia A Factor VIII HGPRT de ciency Lesch Nyhan syndrome Hypoxanthineguanine phosphoribosyltransferase Leigh encephalomyelopathy Pyruvate dehydrogenase E101 Severe combined immunodeficiency Adenosine deaminase Spinal muscle atrophy SMNI or SMNZ PremRNA I In neuronal In thyroid cells cells A A I CGRP Calcitonin Figure 2945 mummy sixm 541mm m mmw mgmwm mum Guanosine binding site 5 Spiced 539 quotm Upstream AW W UAK muvmuww axon Intr f 339 5 539 DH lt G 0quot gt o I I G Downstream K 0quot exon 3 339 gt gt 414 399 395 3 3 m RNA Regula rion of Transcription ac operon Repressors bind DNA ro preven r Tx Ac riva rors bind DNA To s rimula re TX Inducer binding repressor gt releaseDNA Corepressor binding repressor gt bind DNA Lac operon Lac repressor repression relieved by inducer AcTivaTion Processes subject to regulation Gene DNA Transcription Primary W Nucleatides transcript Pns ranszriplionul Frimessing mRNA degradation Mature mRNA W Translation 6 Protein Amino acids inactive 9 D Fusttmnslutionul 39 i Protein a rocessin P g degradation Modified i protein I r active a Win Protein targeting and imnspnn
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