Class Note for CHEM 490 at UMass(23)
Class Note for CHEM 490 at UMass(23)
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This 18 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at University of Massachusetts taught by a professor in Fall. Since its upload, it has received 14 views.
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Date Created: 02/06/15
OxidaTive Phosphoryla39rion Ch 18 Mon 4609 vElec rron Transpor r NADH To 02 creaTes pH gradien r across membrane H flow down gradient drives ATP synThesis PhoTophosphoryla rion VCalculaTing energy of 6 Transfer H Transporf VMiTochondria v Overview of electron Transfer componen rs Proteins amp cofacfors ElecTron Transporf complexes 1mm 43 ATP syn rhase LL39fr C Uncouplers amp inhibi rors W Low EO Hout ATP Carb e39 H ATP transfer transfer synthesis Lots 002 High Eo v ADP Pi NADH H NAD rumame Sucrjnaxe Matrix ATP Chequotital ATP Eledrlzal potemial synthesis potemial ApH driven by Aw inside protonmotive inside alkaline one negative Mitochondrial membrane Complex IV tunCon Cytochrome c Oxidase cotbb Cys quot 5 Live cm 2WMV01 q 4A1up1r7vu10 qua 1Two molecules of cytochrome sequentially 2Reduced CuB and Fe in transfer electrons to reduce CUB and heme a3 heme a3 bind 01 which 2 Cytochrome c forms a peroxide bridge I Jm w CuACuA e 02 e 2 Cytochrome c s ftrwle Paranaa 1 0H 1 ng 4The addition of two more 3The addition of two more k 0 lm protons leads to the electrons and two more protons release of water cleaves the peroxide bridge Figure 1 81 4 Rinrhamhrn cry uh Intermemhrane space F side 3 NADH w Malrix N side 21 91490110 c E Neurospom 3 Saccharomyces E E Screwworm fly g 3 Penguin Chicken Pigeon Du c Kangaroo 3 Wk Rabbit Pig Donkey Horse rubrum MYL 1 kwa rid HM labia mlc at H M M 7 MDquot 5 M Substrate Substrate 24Dinitrophenol 9 2 quot39 u c I 3 a S T g U u a M 191quot a gt gt 5 5 Time ob w L Time wan 39 39 0 Mammy gr AoP I 1 whtkwdnh 10mg mm Uvm39w i of quot1 r d V 5 WW quot4quot A I dialdblc quot 399 13 wmh mus e e 39m H 39 QC0 0 e 0 Z O a 0 N02 i N N N e e e O T o lt gt 5 0 9 O 3 9 Q 3 N02 m N N N 3 K 40 e e e e 24Dlnltrophenol p a 0 0 0 07 o 24Dinitrophenolate anion Figure 144 Principles of Biochemistry Axle emo Jsk b 2006 Pearson Prentice Hall ln H arde I dissgo bo h WinJ 1M 3quot 9 3 WW MWquot F1 ATPase a W Tao9L ATP ADP Pi Figure 1415 Principles of Biochemistry4le 2006 Pearson Prentke Hall Inc aXBx39y complex V i 397 Cr ADW QuickTime M and a Animation decompressor are needed to see this picture Coverslip m Esour e aw in Migunammwu mmmkw 245th Aircd W FLOquot 9 75 moquot w httpwwwrestlteohaopselbutumalnhtmlrIthselbu rOIBCtgf i ehtm ol W w a on Xe sxJL Hm Matrix D Wquot H 3 H haIfihannel StxlhEdmwl 5 MP 2mm and campmy X My WWM Subunh 1 Inner mitochondrial membrane Matrix 39 Phosphate Malate Pyruvate Phosphate Dicarboxylate Tricarboxylate Pyruvate carrier carrier carrier Figure 133 Blurhzmisvy Sixquot Edikian w mm W MWquot und mummy Transport costs 1 H ATP 3HATP 1 for transfer 4HATP synthesized eukaryotes 2e39 transfer NADH to 02 gt 10 H transfer gt 25 ATmeH F pn hmnhs MVP ATP equivalents Glucose 2ATP gt 2 NADH 2 Pyruvate l gt 2 NADH 2 Acetyl CoA Substratelevel phosphorylation K 2 GTP 6 NADH or ATP 2 QH2 4 Total 32 ATP molecules Figure 1310 Principles of Biochemistry 4e 2006 Pearson Prentice Hall Inc ATP equivalents 5 25 AT NADH Membrane associated electron transport plus ATP synthesis 3 S WQHL quotAWk bM rutenone NADH 0 Cyt b Cy c1 Cyt c mind Mnml antimycin A NADH Q Cyt b Cyt C1 Cyt c NADH Q Cyt b Cyt 1 Cyl c an M be nsva Ho 4W 239 yfr Suva 9 7 W 3 5quot Wkquot oak vol4quotquot I V R m 39 Uvlfw 101 Cyt a a3 gt 02 Cyt a a gt 02 CN39 or CO Cyt a a3 gt 02 H fd TABLE 194 Agents That Inte ere with Oxidative Phosphnryiation Type of interference Compound Targetmode at action lnhlbluon of election transfer Cyanide mm memo mime Carbun monoxide Antimycin A Blocks eieclron lransferfrcm cytochrome b In cytochrome c Myxulhiazo Rutenone Prevent eleclmn transfer from FeS centerm ubiquinune Amyial Piericidm A X Inhibitian of ATP synthase Aumvertin Inhibits Fl Oligomycm Venturicidin DCCD Blacks proton ow through F 7 and CFu uncoupling of phosphmylauan FCCF horn eiectron transfer DNP Hydmphomc plumquot Gamers Valinomycin K ionnphnre Thermagenin In brawn lat forms promuccnducmg pales in inner milochondriai membrane x Inhibition aniPADP exchange Mraclylnside InhihiLs adenine nucleoiide lransianase DCMU is Silardicniomphenylirlrldimemylurea DCCD mcycinhexyicarbhdumide FCCP cyaniderpAmiiumomeumxyphenyihydmmnz DNE urmnirmpirenoi X W 0 conw E Vquot A11 1wa3 mxsvr
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