Class Note for CHEM 490 at UMass(24)
Class Note for CHEM 490 at UMass(24)
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This 11 page Class Notes was uploaded by an elite notetaker on Friday February 6, 2015. The Class Notes belongs to a course at University of Massachusetts taught by a professor in Fall. Since its upload, it has received 19 views.
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Date Created: 02/06/15
Processing nicuc eoiides Base modificai ion iQNi i rRNA mRN i processing in eukaryoies 5 cap and Bipoy id enhance siabiiiiy Spiicing om in i rons reiain axons Regula rion of Transcription ac operon Repressors bind DNA ro preven r Tx Ac riva rors bind DNA To s rimula re TX Inducer binding repressor gt releaseDNA Corepressor binding repressor gt bind DNA Lac operon Lac repressor repression relieved by inducer Mon 42709 Processes subject to regulation Gene DNA Transcription h Prim wvvvvw Nucleotides transcript Fns runszripliunul protesslng mRNA degradation Mature mRNA W Translation Protein Amino acids inactive 0 Pus ranslutionul 39u39u Protein 1 rocessin P g degradation Mudified protein 3 V active if Protein targeting and transport Control A Regmator sites Structural genes gene far 4 1 B Lactose operon mumw HFlremnndnd mummy Activator stimulates transcription Activator RNA polymerase D NA b w Activator A 5 r 0 Ligand O Ligand 1 O a j W i v 5 An activator with bound ligand An activator stimulates transcription In the stimulates transcription presence of ligand the activator is inhibited um Flgule 11gtquot Printlplesnmlnmemlslmue 39 m m5 Peanut Pumice Hill wFfen ligandbound OR when ligandfree L ooactivator Repressor prevents transcription Repressor 0 Ligand a r o Ligand 8 V In the absence of ligand the repressor does not bind to DNA Repression occurs only when ligand 39 corepressor is present 1 mm mammwm u 5 Emymumwwm quot A repressor prevents transcription Binding of ligand inducer to the repressor inactivates the repressor and allows transcription mm u liivhen ligandfree OR when ligandbound L inducer L oorepressor HO BGalactosidase pg CHZOH CHZOH H20 0 H OH O OH 5 BGalactosidase OH lndose Lactose removed 4 2 Ladose added 0 30 60 90 Total bacterial protein 9 CHZOH CHIOH 0 0H 0 0 OH OH HO OH OH OH Gulmlose Glucose W i mmu Repressor bound to operator 1 site prevents transcription of z y and a Lactose Galadoside Outside permease p i 7 WM 3 Iquot H r H I quotgt W Insde mmm Repressor bound to operator cHoH canon 11 site prevents transcription HO H H 0 ofz and a bw w I y H on H on quotmm mm Hmquot Lactose mum l galauosidase 0 CH2 39 man an mRNA H H OH H OH NO H AllulacmSE H Oquot tlnducer o 3 Gaactosidase Permease Transacetylase HIGH HO H DH H H on Repressorinducer H quot H Ho H complexdoes not H OH H OH bind DNA Galactosa Glucose hams n Hm mnmm mm m mm Lactose Galadoside rpevmease Oulside CH10H H OH H OH Lactose l galaunsidase cl cH2 H quot quot CHZOH Allalactns H OH 0 CH3 0quot H s c H I Hon OH H CH3 Ho H DH H H H OH H OH H H H HO H H H H oH H o Galaduse Glucose lsopropylthlogalactonde IPTG 5 TGTGTGGAATTGTGAGC aGATAACAATTTCACACA 3 3 ACACACCTTAAC ACTCGCCTAATGTTAAAGTGTGT 5 Operator DNA K 1 la repressor 15 Arg a helix Figure 31 gt2 Hinzhzmv39svy Sim waww MW Major groove binding H 0 I R N C C R 39139 I H R N c C R39 Arglnlne lmz CH2 H CH2 Glutamlne I CH2 orasparagine CH2 TH C 0 N HN S H N H CH3 H H H H OlnH I N N 391 N7 7 Hmo H39quot 5 N N N Nk N N Nquot39 6 7 7 0 N X N OI gt H N N H ThymineAdenine CytosineEGuanine
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