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how to calculate amino acid

by: Olubunmi Olakunle

how to calculate amino acid CHEM 357

Marketplace > Chemistry > CHEM 357 > how to calculate amino acid
Olubunmi Olakunle
Biochemistry II
Dr. Ana Soto

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About this Document

It covers how to calculate the amino acid using those equation.
Biochemistry II
Dr. Ana Soto
Class Notes
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This 0 page Class Notes was uploaded by Olubunmi Olakunle on Monday February 1, 2016. The Class Notes belongs to CHEM 357 at a university taught by Dr. Ana Soto in Spring 2016. Since its upload, it has received 5 views.


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Date Created: 02/01/16
Molecular e of Domain 3 using ExPasy and hand calculation Number of amino acids 226 Molecular weight 246335 Theoretical pl 544 Amino acid composition Ala A 23 102 Arg R 18 80 Asn N 3 13 Asp D 14 62 Cys C 0 00 Gln Q 9 40 Glu E 16 71 Gly G 25 111 His H 9 40 lie I 11 49 Leu L 22 97 Lys K 3 13 Met M 1 04 Phe F 7 31 Pro P 19 84 Ser S 12 53 Thr T 11 49 Trp W 3 13 Tyr Y 8 35 Val V 12 53 Pyl O 0 00 Sec U 0 00 B O 00 Z O 00 X 0 00 Total number of negatively charged residues Asp Glu 30 Total number of positively charged residues Arg Lys 21 Atomic composition Carbon C 1098 Hydrogen H 1694 Nitrogen N 316 Oxygen O 330 Sulfur S 1 Formula C1098H1694N316033051 Total number of atoms 3439 Extinction coef cients Extinction coef cients are in units of M1 cm1 at 280 nm measured in water Ext coef cient 28420 Abs 01 1 gl 1154 Estimated halflife The Nterminal of the sequence considered is M Met The estimated halflife is 30 hours mammalian reticulocytes in vitro gt20 hours yeast in vivo gt10 hours Escherichia coli in vivo Instability index The instability index II is computed to be 4150 This classi es the protein as unstable Aliphatic index 8252 Grand average of hydropathicity GRAW 0411 HAND CALCULATION 3550081490012528420 M39lcm39l


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