Chapter 12 exam 2
Chapter 12 exam 2 PCB 4522
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This 2 page Class Notes was uploaded by Dylan Diljohn on Thursday February 4, 2016. The Class Notes belongs to PCB 4522 at University of Florida taught by William Gurley in Spring 2016. Since its upload, it has received 44 views. For similar materials see Molecular Genetics in Microbiology at University of Florida.
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Date Created: 02/04/16
Dylan Diljohn University of Florida PCB 4522: Molecular Genetics Exam 2: Chapter 12 Ti plasmids= causes gall disease in plants A. tumefaciens (agrobacterium) can turn plant cells into tumors o Ti plasmid (infectious agent) is carried by the A. tumefaciens (bacterium) Agrobacterium Agrobacterium only infects wounded plants o It transfer T-DNA in the wounds site (the tumors) Acetosyringone is produce by the wounded plant cells (phenolic cmpd) o Vir genes are induced by the phenolic cmpd (acetosyringone), which are expelled from the plant at the wound site. Opines is produce by the tumor and Agrobacterium, in the soil, uses it as a nutrient Ti Plasmid components T-DNA: o Agrobacterium transfer T-DNA to the plant and integrate into the plants’ chromosome (only the T-DNA!) o Functions: Opine syn Plant hormone syn Vir gene (virulence region): o Encodes for the transfer the T-DNA (single strandedlater ds once integrated in the plants genome) o Located outside the transferred region o Vir A,B,G,C,D,E Opine catabolism: o Bacteria are able to use octopine and noplaine nutrient (most soil bacteria lack these genes) Bacterial conjugation (tra): o For the transfer of the pTi btw different Agrobacterium Genes are Arginine derivative (use by the tumor cells) o Shi (Funt: shoot induction) & Roi (Funt: root induction)= hormone syn o Nos (Funt: nopaline syn) & Ocs (Funt: octopine syn)= opine syn Vir Gene VirA: o a receptor for acetosyringone. “always being express (turn on)” o Autophosphorylated on histidine (once bound with inducer) o Activates VirG: by transferring phosphate group VirG: o Positive regulator for other vir genes Increases activity once activated, causes its own promoter to have a new start point 1 Dylan Diljohn University of Florida PCB 4522: Molecular Genetics VirD1/VirD2: o Pilot protein o D1:Recognizing the nicking site (right boundary) o D2:Nicks the DNA stand and binds at 5’ end (right boundary) Contain 2 NLS seq o After nick a primer is created to make a new DNA strand o Termination: when DNA syn reach the left boundary o Transferred to the nucleus VirE2 o SSBP (single-stranded binding protein) o Helps with transferring DNA o Transferred to the nucleus o Contain NLS 2
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