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by: Kayla Notetaker

Microscopy Bio 306

Kayla Notetaker

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About this Document

How to work a light microscope
General Microbiology
Dr. Jim Burritt
Class Notes
25 ?




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"What an unbelievable resource! I probably needed course on how to decipher my own handwriting, but not anymore..."

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This 1 page Class Notes was uploaded by Kayla Notetaker on Friday February 5, 2016. The Class Notes belongs to Bio 306 at University of Wisconsin - Stout taught by Dr. Jim Burritt in Winter 2016. Since its upload, it has received 27 views. For similar materials see General Microbiology in Biology at University of Wisconsin - Stout.


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Star Star Star Star Star

What an unbelievable resource! I probably needed course on how to decipher my own handwriting, but not anymore...



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Date Created: 02/05/16
Kayla Christianson Lab 1/21/16 12:20-2:20 A microscope is a vital piece of technology for many branches of science. A microscope is a very complex machine and requires a rather detailed understanding of how it works to be able to use it properly. When using a microscope, handling is important. To properly handle the microscope, grip the handle with one hand and place the other hand underneath the microscope until you can feel the rubber feet and it is resting comfortably on that hand, then be sure that the eyepiece is facing towards your chest while transporting the microscope. When it comes to actually using the microscope, there is a series of steps that is important to follow to get the proper results. Lower the stage all the way down and make sure you have the smallest objective in position (4X) before placing the slide into position on the stage. Once the slide is in place, you can move the stage up and down to focus in on the sample. Once the sample has been found then adjusting the light can help make it clearer. The light is able to be adjusted by the diaphragm and the condenser; the diaphragm controls the amount of light that is able to enter the condenser by layers of plates, and the condenser is the lens that focuses the light from the large light source into a more concentrated light underneath the slide. When it comes to the light, you can adjust the fine focus knob which adjusts the contrast of which you are looking at and determining the difference between the background and actual material. There is also something called resolution, which is a skill that is important to master, and that is just how well you can tell the difference of two objects from each other. The level of which you are seeing is called total magnification, which is what you get when you multiply the eyepiece power by the objective lens that is being used. The slides that we looked at today under the microscope were a stained sample of a mouse lung and a wet mount sample of a honey bee. The stained sample had a lot of purple blobs and smears of varying sizes, some a very dark purple and others almost a pink. The wet mount sample had a lot of different colored circles. There was also a lot of yellow dots, which means that a large amount of pollen was also extracted. In the wet mount sample we found feather looking things, which I guessed to be the bees hairs and it turned out I was right so that was pretty cool! Unfortunately we did not see any nosemas in any of our samples.


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