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Week 3 Notes

by: Marissa Reyes-Hernandez

Week 3 Notes 2330

Marissa Reyes-Hernandez
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These notes are from week 3!!!
Microorganisms and Disease - 18884
Roshanak Jafari
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This 4 page Class Notes was uploaded by Marissa Reyes-Hernandez on Monday February 8, 2016. The Class Notes belongs to 2330 at University of Texas at El Paso taught by Roshanak Jafari in Spring 2016. Since its upload, it has received 141 views. For similar materials see Microorganisms and Disease - 18884 in Science at University of Texas at El Paso.


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Date Created: 02/08/16
What is the use of any kind of microscope that uses visible light to observe specimens? light microscopy In a ______ microscope, the image from the objective lens is magnified again by the ocular lens. compound Total magnification = objective lens x ocular lens What is the ability of a lense to distinguish fine detail and structure between two points? Resolution Do shorter or longer wavelengths of light provide greater resolution? shorter What is the measure of light bending ability of a medium? refractive index What is used to keep light from bending? immersion oil Who was it that realized oil has the same refractive index as glass? Ernst Karl Abbe What is it when dark objects are visible against a bright background? brightfield illumination Light objects are visible against a dark background... darkfield illumination In brightfield illumination, does light reflected off the specimen enter the objective lens or not enter the objective lens? does not In darkfield illumination, does light reflected off the specimen enter the objective lens or not enter the objective lens? does enter In what microscopy can you view detailed examination of internal structures in living microorganisms? phase contrast microscopy What is the ability of substances to absorb short wavelengths of light and give off light at a longer wavelength? fluorescence What two types of microscopy manipulate electrons for the image? phase contrast microscopy and differential interference contrast microscopy How many beams of light does differential interference contrast microscopy use compared to phase-contrast microscopy? 2 What type of microscopy uses UV light and fluorescent substances? fluorescence In fluorescence microscopy, what are the organisms stained with? Fluorescein Fluorescence microscopy is used for _____ differences metabolic In which microscopy is short wavelength (blue) light used to excite the dyes? confocal Confocal microscopy is up to ____ um deep 100 In which microscopy is long wavelength (red) light used to excite the dyes? two photon Which microscopy is used to study cells attached to the surface? two photon Two - photon microscopy is up to ___ mm deep 1 What microscopy uses electrons instead of light? electron Does shorter or longer wavelength of electrons give greater resolution? shorter Which microscopy uses ultrathin sections of specimens? transmission electron (TEM) What microscopy uses a beam of electrons to scan the whole surface of a specimen? scanning electron (SEM) What type of microscopy uses a metal-and-diamond probe inserted into the specimen and also produces 3 dimensional images? atomic force (AFM) What microscopy uses a metal probe to scan a specimen and can be used to see atoms? scanning tunneling (STM) Who made the first compound microscope around 1600? Zaccharias Janssen Specimens must be made to contrast sharply with their what? medium What do we use to preserve the direction of light rays at the highest magnification? immersion oil Is the resolution higher in a differential interference contrast microscope or a phase contrast microscope? differential interference contrast microscope The wavelengths of electrons compared to the wavelengths of visible light are how many times smaller? 100,000 What do electron microscopes use to focus a beam of electrons onto a specimen? electromagnetic lenses What is the final image in an TEM called?


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