New User Special Price Expires in

Let's log you in.

Sign in with Facebook


Don't have a StudySoup account? Create one here!


Create a StudySoup account

Be part of our community, it's free to join!

Sign up with Facebook


Create your account
By creating an account you agree to StudySoup's terms and conditions and privacy policy

Already have a StudySoup account? Login here

Exam 2: Chapter 15 part 1 Recombination

by: Dylan Diljohn

Exam 2: Chapter 15 part 1 Recombination PCB 4522

Marketplace > University of Florida > Microbiology > PCB 4522 > Exam 2 Chapter 15 part 1 Recombination
Dylan Diljohn
Molecular Genetics
William Gurley

Almost Ready


These notes were just uploaded, and will be ready to view shortly.

Purchase these notes here, or revisit this page.

Either way, we'll remind you when they're ready :)

Preview These Notes for FREE

Get a free preview of these Notes, just enter your email below.

Unlock Preview
Unlock Preview

Preview these materials now for free

Why put in your email? Get access to more of this material and other relevant free materials for your school

View Preview

About this Document

Exam 2: Chapter 15 part 1 Recombination
Molecular Genetics
William Gurley
Class Notes
PCB4522, PCB 4522, Molecular Genetics, Genetics, Gurley, Gurley William, Dr. Gurley William, UF, University vof Florida
25 ?




Popular in Molecular Genetics

Popular in Microbiology

This page Class Notes was uploaded by Dylan Diljohn on Monday February 8, 2016. The Class Notes belongs to PCB 4522 at University of Florida taught by William Gurley in Spring 2016. Since its upload, it has received 17 views. For similar materials see Molecular Genetics in Microbiology at University of Florida.


Reviews for Exam 2: Chapter 15 part 1 Recombination


Report this Material


What is Karma?


Karma is the currency of StudySoup.

You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!

Date Created: 02/08/16
Dylan Diljohn University of Florida PCB 4522 Molecular Genetics Exam 2 Chapter 15 part 1 0 Recombination Evolution genetic recombination mutation 0 Separate Favorable form unfavorable shuf ing genes Types of Recombination 0 Homologous O Males meiosis 0 Female in the formation of gametes 0 Sitespecific phage integration 0 Bacterium host chromo 0 Transposition specific enzymes are use 0 Immune sys shuf ing and mating type switch in yeast At meiosis only 2 of the 4 chromatids are affected undergo recombination not all 4 at once 0 So a single recombinant event Will produce a 50 recombinants HR homologous recombination occurs btW synapsed chromo recombinant jointscrossing overchiasma of the gametes Females lst Meiotic Prophase 0 Leptotene 0 DNA rep is has haulted O Chromo are visible 0 Zygotene 0 Pairing begins in chromos synapsis homologous pairing 0 Pachytene O Synaptonemal complex SC complete 0 Recombination is complete 0 Diplotene O Chromos are separated but held together by chiasmata Diakinesis O Chromo condense detach from envelope 0 Chaismata are visible Dylan Diljohn University of Florida PCB 4522 Molecular Genetics 1DoubleStrand Break Repair meiosis 0 DSBDS ends resection cutting 5 endsss invasion of DNA Dlooprecombinant jointljbranch migrationsynthesis of both DNA strandsIlligationResolutionDSB repair W crossing over or Wout crossing over 0 Most common in HR 0 5 end capture patch or crossover 0 Resolution of HR 2 O Crossing Recombinant vertical cut two outer strands 0 Patch Recombinant horizontal cut two inner strands Spoll protein that generate meiotic DSB double strand break 0 Covalently attached to 5 end 0 Topoisomerase 2 family HRhybrid DNA 0 44 ratio normal crossover 0 35 ratio hybrid DNA that s not repaired 0 26 ratio hybrid DNA that s repaired 2 Mitotic recombination SDSA O Discovered in yeast mating type switch 0 First steps are the same DSB resectionstrand invasionEISDSAsynthesisdep strand annealing in DSB repair model except 1 No 2nd end capture 2 No holiday structure formed 3 No crossing over 3 NHEJ nonhomologous endjoining can repair DSB 0 Happens in the G1 phase I the cell cycle 0 Important in the immune sys shuf ing 0 Requires the entering of the Ku protein Ku70 and Ku80 at the DSB 0 Insertiondeletion mutation are created to the ligation step 0 XRCC4LigIV enzyme that are recruited and fills gap 0 Involves in antibody gene rearrangements Dylan Diljohn University of Florida PCB 4522 Molecular Genetics 4 SSA Singlestrand annealing DSB repair 0 No DLoop 0 DSB repeat seq anneal to bring the ends together eleimination of nonrepeating seq btW the two repeats 0 DSBDannealing lligation 3 tail 0 Repeats involves 0 Intervening seq deleted 0 No strand invasion 0 No cross over 5 BIR BreakInduced Replication repair ds ends 0 Repair nonhomologus chromo ends that has a repeated seq 5 BIR repair ds break 0 Annealing repeated seq on nonhomologous chromo 0 No end capture Synaptonemal complexes 0 Begin forming in late Leptotene Forms after DSB 0 Homologous chromo re paired in the synaptonemal complex SC 0 Block SC 0 Rate of recombination is less distance crossover is no longer controlled 0 DSB blocks formation of SC 0 Rad50 mutation 0 Blocks the formation of 3 ss end 0 Recombination would not occur since 3 end at the DSB would not be processed O Prevent SC formation Processing of the ds break 1 Exonuclease activity a Create 5 recessed end rab50 protein 2 Strand invasion a Dcml humans protein b Rad51 yeast protein 2 indep phase of meiotic recombination 0 Phase lzDSBrepair Dylan Diljohn University of Florida PCB 4522 Molecular Genetics 0 Phase 2 SDSAsynthesis dep strand annealing 0 Binds to protein present in early recombination nodules and initiates formation of the SC 0 In rad50 mutant not spoll mutant 0 Localized at the axial associations and DSB breaks are a requirement 0 Can tell btW homologous or nonhomologous the protein just go the DSB 0 Initiate formation of the SC Zip2 and Zipl mutant 0 Forms at axial associations but no SC 0 Zip2 is recruit before Zipl at DSB Hop2 mutant 0 Form SC but contain nonH chromo 0 Don t pair chromo Hop2 control chromo pairing MSH4mismatch repair Dmcl and Rad51 homologs of RecA Meiosis is block if no crossover occur cells do not segregate


Buy Material

Are you sure you want to buy this material for

25 Karma

Buy Material

BOOM! Enjoy Your Free Notes!

We've added these Notes to your profile, click here to view them now.


You're already Subscribed!

Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'

Why people love StudySoup

Steve Martinelli UC Los Angeles

"There's no way I would have passed my Organic Chemistry class this semester without the notes and study guides I got from StudySoup."

Anthony Lee UC Santa Barbara

"I bought an awesome study guide, which helped me get an A in my Math 34B class this quarter!"

Bentley McCaw University of Florida

"I was shooting for a perfect 4.0 GPA this semester. Having StudySoup as a study aid was critical to helping me achieve my goal...and I nailed it!"


"Their 'Elite Notetakers' are making over $1,200/month in sales by creating high quality content that helps their classmates in a time of need."

Become an Elite Notetaker and start selling your notes online!

Refund Policy


All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email


StudySoup has more than 1 million course-specific study resources to help students study smarter. If you’re having trouble finding what you’re looking for, our customer support team can help you find what you need! Feel free to contact them here:

Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to

Satisfaction Guarantee: If you’re not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.

Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.