Exam 2: Chapter 15 part 1 Recombination
Exam 2: Chapter 15 part 1 Recombination PCB 4522
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This page Class Notes was uploaded by Dylan Diljohn on Monday February 8, 2016. The Class Notes belongs to PCB 4522 at University of Florida taught by William Gurley in Spring 2016. Since its upload, it has received 17 views. For similar materials see Molecular Genetics in Microbiology at University of Florida.
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Date Created: 02/08/16
Dylan Diljohn University of Florida PCB 4522 Molecular Genetics Exam 2 Chapter 15 part 1 0 Recombination Evolution genetic recombination mutation 0 Separate Favorable form unfavorable shuf ing genes Types of Recombination 0 Homologous O Males meiosis 0 Female in the formation of gametes 0 Sitespecific phage integration 0 Bacterium host chromo 0 Transposition specific enzymes are use 0 Immune sys shuf ing and mating type switch in yeast At meiosis only 2 of the 4 chromatids are affected undergo recombination not all 4 at once 0 So a single recombinant event Will produce a 50 recombinants HR homologous recombination occurs btW synapsed chromo recombinant jointscrossing overchiasma of the gametes Females lst Meiotic Prophase 0 Leptotene 0 DNA rep is has haulted O Chromo are visible 0 Zygotene 0 Pairing begins in chromos synapsis homologous pairing 0 Pachytene O Synaptonemal complex SC complete 0 Recombination is complete 0 Diplotene O Chromos are separated but held together by chiasmata Diakinesis O Chromo condense detach from envelope 0 Chaismata are visible Dylan Diljohn University of Florida PCB 4522 Molecular Genetics 1DoubleStrand Break Repair meiosis 0 DSBDS ends resection cutting 5 endsss invasion of DNA Dlooprecombinant jointljbranch migrationsynthesis of both DNA strandsIlligationResolutionDSB repair W crossing over or Wout crossing over 0 Most common in HR 0 5 end capture patch or crossover 0 Resolution of HR 2 O Crossing Recombinant vertical cut two outer strands 0 Patch Recombinant horizontal cut two inner strands Spoll protein that generate meiotic DSB double strand break 0 Covalently attached to 5 end 0 Topoisomerase 2 family HRhybrid DNA 0 44 ratio normal crossover 0 35 ratio hybrid DNA that s not repaired 0 26 ratio hybrid DNA that s repaired 2 Mitotic recombination SDSA O Discovered in yeast mating type switch 0 First steps are the same DSB resectionstrand invasionEISDSAsynthesisdep strand annealing in DSB repair model except 1 No 2nd end capture 2 No holiday structure formed 3 No crossing over 3 NHEJ nonhomologous endjoining can repair DSB 0 Happens in the G1 phase I the cell cycle 0 Important in the immune sys shuf ing 0 Requires the entering of the Ku protein Ku70 and Ku80 at the DSB 0 Insertiondeletion mutation are created to the ligation step 0 XRCC4LigIV enzyme that are recruited and fills gap 0 Involves in antibody gene rearrangements Dylan Diljohn University of Florida PCB 4522 Molecular Genetics 4 SSA Singlestrand annealing DSB repair 0 No DLoop 0 DSB repeat seq anneal to bring the ends together eleimination of nonrepeating seq btW the two repeats 0 DSBDannealing lligation 3 tail 0 Repeats involves 0 Intervening seq deleted 0 No strand invasion 0 No cross over 5 BIR BreakInduced Replication repair ds ends 0 Repair nonhomologus chromo ends that has a repeated seq 5 BIR repair ds break 0 Annealing repeated seq on nonhomologous chromo 0 No end capture Synaptonemal complexes 0 Begin forming in late Leptotene Forms after DSB 0 Homologous chromo re paired in the synaptonemal complex SC 0 Block SC 0 Rate of recombination is less distance crossover is no longer controlled 0 DSB blocks formation of SC 0 Rad50 mutation 0 Blocks the formation of 3 ss end 0 Recombination would not occur since 3 end at the DSB would not be processed O Prevent SC formation Processing of the ds break 1 Exonuclease activity a Create 5 recessed end rab50 protein 2 Strand invasion a Dcml humans protein b Rad51 yeast protein 2 indep phase of meiotic recombination 0 Phase lzDSBrepair Dylan Diljohn University of Florida PCB 4522 Molecular Genetics 0 Phase 2 SDSAsynthesis dep strand annealing 0 Binds to protein present in early recombination nodules and initiates formation of the SC 0 In rad50 mutant not spoll mutant 0 Localized at the axial associations and DSB breaks are a requirement 0 Can tell btW homologous or nonhomologous the protein just go the DSB 0 Initiate formation of the SC Zip2 and Zipl mutant 0 Forms at axial associations but no SC 0 Zip2 is recruit before Zipl at DSB Hop2 mutant 0 Form SC but contain nonH chromo 0 Don t pair chromo Hop2 control chromo pairing MSH4mismatch repair Dmcl and Rad51 homologs of RecA Meiosis is block if no crossover occur cells do not segregate
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