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Microbiology notes

by: UNT_Scientist

Microbiology notes Biol 2041


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About this Document

This is the last of the notes for the test 1
Daniel Kunz
Class Notes
Microbiology, notes, biol 2041
25 ?




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This 3 page Class Notes was uploaded by UNT_Scientist on Wednesday February 10, 2016. The Class Notes belongs to Biol 2041 at University of North Texas taught by Daniel Kunz in Winter 2016. Since its upload, it has received 28 views. For similar materials see Microbiology in Biology at University of North Texas.


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Date Created: 02/10/16
Microbiology notes  ● Enzymes   ○ Speed up chemical reactions   ○ Types  ■ Oxidation   ● Removal of electron   ● Aka take hydrogen away  ● Also know as dehydrogenation   ■ Reduction   ● Gain electron   ● Aka take Oxygen away  ■ Redox reaction   ● Oxidation Paris with reduction   ● 5.9 figure  ■ Cofactors   ● Things used to activate an enzyme before  the substrate   ■ Dehydrogenases   ● Needs a protein  & a cofactor (nonprotein)  and then a substrate forming a holoenzyme  (whole enzyme)  ○ If it's tightly bound its know as a  prosthetic group   ■ Doesn't like to leave   ● Figure 5.3  ○ Denaturation   ■ Loss of tertiary structure isn't the primary structure   ■ Happens due to temperature and pH  ● Conservation of energy   ○ Mechanisms of generating ATP   ■ Substrate­level phosphorylation   ● P­diphosphoglyceric acid­P ­­­>  3­phosphoglyceric acid  ○ 1,3­DPGA­­­>3­PGA  ■ Oxidative phosphorylation   ● Figure 5.14  ● Involves ETC  ○ Electron transport chain   ■ Bound to the mitochondrial  membrane  ■ In bacterial this occurs on the  plasma membrane  ○ Enzymatic complexes   ■ NADH dehydrogenase  (flavoprotein)  ■ Cytochrome b­1​(Fe)  ■ Cytochrome oxidases (Fe)  ■ Photophosphorylation   ● Light­driven  ○ Glycolysis   ■ 4 ATP produced   ■ 2NADH produced     ● Aerobic respiration   ■ C6​ 12​ 6​62​­­> 6C2​ 6H2​  ■ Emben­Meyerhof pathway  ○ Pre­TCA  ■ Occurs in cytoplasm   ■ Everything after occurs in the mitochondria   ○ KRebs cycle, TCA   ■ Fig 5.13  ■ produces ATP via oxidative phosphorylation   ■ Malic acid  picture   ○ Table 5.3 keeps track of the energy bookkeeping   ○ Chemiosmosis   ■ Peter Mitchell Nobel 1978  ■ . Fig 5.16  ■ Yields 3 ATP per 1 NADH  ● What if there is no oxygen?  ○ Glucose fermentation  ■ Yeast   ■ Forms lactic acid and or ethanol  ■ only forms via substrate level phosphorylation  ■ fig 5.18b  ■ Wiseman process  ● How we make acetone  ■ Fig 5.27  ■ Doesn't use electron transport chain   ○ E.coli can live theses ways:  ■ Aerobic   ● Glucose   ○ Electron donor   ● NH​3   ● S  ● P  ● TM  ● O  ○ Electron acceptor   ■ Anaerobic  ● Glucose   ○ Electron donor   ­   ● NO​3​ ● S  ● P  ● TM   ● He  ○ Electron acceptor   ■ Fermentation   ● Glucose   ○ Electron donor  ● N​­3   ● S  ● P  ● TM  ● He   ○ Electron acceptor 


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