New User Special Price Expires in

Let's log you in.

Sign in with Facebook


Don't have a StudySoup account? Create one here!


Create a StudySoup account

Be part of our community, it's free to join!

Sign up with Facebook


Create your account
By creating an account you agree to StudySoup's terms and conditions and privacy policy

Already have a StudySoup account? Login here

Genomes & Replication

by: Mallory McMullen

Genomes & Replication 4100

Marketplace > Ohio State University > Microbiology > 4100 > Genomes Replication
Mallory McMullen

Preview These Notes for FREE

Get a free preview of these Notes, just enter your email below.

Unlock Preview
Unlock Preview

Preview these materials now for free

Why put in your email? Get access to more of this material and other relevant free materials for your school

View Preview

About this Document

Dr. Daniels' lecture notes from Wednesday February 10- Friday February 12
Charles Daniels
Class Notes
25 ?




Popular in Microbiology

Popular in Microbiology

This 2 page Class Notes was uploaded by Mallory McMullen on Friday February 12, 2016. The Class Notes belongs to 4100 at Ohio State University taught by Charles Daniels in Fall. Since its upload, it has received 31 views. For similar materials see Microbiology in Microbiology at Ohio State University.


Reviews for Genomes & Replication


Report this Material


What is Karma?


Karma is the currency of StudySoup.

You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!

Date Created: 02/12/16
Friday, February 12, 2016 Genomes & Replication ▯ Wednesday Feb. 10- Friday Feb. 12 - Genome: all of the genetic information present in a cell including chromosomes, plasmids, and prophage ▯ - Prokaryotes use vertical inheritance (parent to child) and horizontal (conjugation)▯ - Operon: a unit encoding multiple genes▯ - Regulon: multiple transcription units (genes or operons) controlled by a single regulatory molecule▯ - Base + sugar= nucleoside; base + sugar + phosphate= nucleotide ▯ - The sugar backbone in DNA is deoxyribose; in DNA it’s ribose (extra alcohol)▯ - The major groove in the double helix can be a site for recognition proteins in DNA▯ - Nucleoid: series of loops/domains that supercoil bacterial DNA▯ Anchored by histone-like proteins ▯ • - If a strand breaks and rejoins on the other side, it will wind counter-clockwise to relieve the stress and become negatively supercoiled by topoisomerases ▯ • Type 1: single protein that cleaves one strand and rejoins it in the right place▯ Type 2: multiple subunit that uses ATP to introduce negative supercoils ▯ • - e.g. Gyrase- said to “relieve” positive supercoils ▯ - Chromosomal replication moves two replication forks in opposite directions ▯ - Chain growth is in the 5’-3’ direction with a template strand, a terminal -OH on the growing strand, and dNTPs (deoxyribonucleotide triphosphates)▯ - Each replication fork has a leading continuous strand and a lagging strand with intermediate Okazaki fragments of short RNA-DNA▯ - There is an error rate of only 1 in 10^8 with proofreading▯ - Ribonuclease hybrid: removes RNA primers ▯ • Then DNAP I synthesizes a DNA patch using the terminal -OH as a priming site, then ligase repairs the phosphodiester nick using ATP▯ 1 Friday, February 12, 2016 - DNA polymerase III runs only in the 3’-5’ direction; DNAP I does both for repair ▯ - Archaea genome replication is similar to eukaryotes ▯ - The start of replication is triggered by the ratio of DNA to cell mass ▯ - Replisome: two DNAP III with primase and helicase▯ • Primase acts as short RNA primer that provides the needed terminal -OH so the lagging strand can be synthesized in steps because as synthesis takes place in the 5’-3’ direction the replication fork moves along ▯ - Tus: (terminus utilization substance) binds to terminator sequences and acts as a counter-helicase ▯ - Plasmid: circular DNA molecules that replicate separately from the chromosomes▯ • Code for gene products that offer a selective advantage (non-essential)▯ • Can replicate bidirectionally (starts at a single origin and goes out in 2 directions) or rolling-circle (starts at a single origin and moves in one direction)▯ • Usually rely on the host for polymerase, ligase, primase, and helicase 2


Buy Material

Are you sure you want to buy this material for

25 Karma

Buy Material

BOOM! Enjoy Your Free Notes!

We've added these Notes to your profile, click here to view them now.


You're already Subscribed!

Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'

Why people love StudySoup

Bentley McCaw University of Florida

"I was shooting for a perfect 4.0 GPA this semester. Having StudySoup as a study aid was critical to helping me achieve my goal...and I nailed it!"

Amaris Trozzo George Washington University

"I made $350 in just two days after posting my first study guide."

Steve Martinelli UC Los Angeles

"There's no way I would have passed my Organic Chemistry class this semester without the notes and study guides I got from StudySoup."

Parker Thompson 500 Startups

"It's a great way for students to improve their educational experience and it seemed like a product that everybody wants, so all the people participating are winning."

Become an Elite Notetaker and start selling your notes online!

Refund Policy


All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email


StudySoup has more than 1 million course-specific study resources to help students study smarter. If you’re having trouble finding what you’re looking for, our customer support team can help you find what you need! Feel free to contact them here:

Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to

Satisfaction Guarantee: If you’re not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.

Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.