Biochemistry Week 5
Biochemistry Week 5 CHEM 3653 - 001
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This 1 page Class Notes was uploaded by Lauran Notetaker on Sunday February 21, 2016. The Class Notes belongs to CHEM 3653 - 001 at University of Oklahoma taught by Dr. Paul A. Sims in Winter 2016. Since its upload, it has received 23 views. For similar materials see Biochemistry in Chemistry at University of Oklahoma.
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Date Created: 02/21/16
February 16, 2016 Flow of genetic info in normal cells is: “Central Dogma” DNA RNA Protein transcription translation 4-Hydroxyproline formation of collagen (connective tissue) - bleeding gums Histones rich in lysosomes Peptide bond - partial double bond planar, constrains how a polypeptide can fold Hydrolysis is exergonic As pH INCREASES the overall charge on a peptide will become more NEGATIVE As pH DECREASES the overall charge on a peptide will become more POSITIVE n Net Charge: (Sigma)qi ﬁ i = 1 monomaric - one sub chain Know where trypsin (c-side), chymotrypsin (c-side after tryptophan), and carboxypeptidase (chews way from C terminal) Cleavage with a chemical reagent: Cyanogen Bromide Edman Degradation: about 50 residues doesn't delete whole sample, only hydrolyzes peptide on end February 18, 2016 Exam 1
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