New User Special Price Expires in

Let's log you in.

Sign in with Facebook


Don't have a StudySoup account? Create one here!


Create a StudySoup account

Be part of our community, it's free to join!

Sign up with Facebook


Create your account
By creating an account you agree to StudySoup's terms and conditions and privacy policy

Already have a StudySoup account? Login here

CHEM-C127 Milk Separation Lab Notes

by: Kathryn Brinser

CHEM-C127 Milk Separation Lab Notes Chemistry C127

Marketplace > Indiana University > Chemistry > Chemistry C127 > CHEM C127 Milk Separation Lab Notes
Kathryn Brinser
GPA 4.0

Preview These Notes for FREE

Get a free preview of these Notes, just enter your email below.

Unlock Preview
Unlock Preview

Preview these materials now for free

Why put in your email? Get access to more of this material and other relevant free materials for your school

View Preview

About this Document

These notes cover the lecture material in preparation for the separation of milk lab, including the separation diagram.
Principles of Chemistry and Biochem I Lab
Dr. Norman Dean
Class Notes
chem-c127, chem c127, Chemistry, Chem, c127, milk separation, lab
25 ?




Popular in Principles of Chemistry and Biochem I Lab

Popular in Chemistry

This 2 page Class Notes was uploaded by Kathryn Brinser on Monday February 22, 2016. The Class Notes belongs to Chemistry C127 at Indiana University taught by Dr. Norman Dean in Fall 2016. Since its upload, it has received 645 views. For similar materials see Principles of Chemistry and Biochem I Lab in Chemistry at Indiana University.


Reviews for CHEM-C127 Milk Separation Lab Notes


Report this Material


What is Karma?


Karma is the currency of StudySoup.

You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!

Date Created: 02/22/16
C127 Lecture 7 Notes- Milk Separation 2-25-16  Exam questions relatively evenly split between 4 experiment o Any kind of calculator o Lab notebook o 3x5 note card allowed o Sunday 7:15 Q/A review  FIRST FORMAL LAB REPORT  Learning Goals o Perform natural product separation o Identify major components of milk o Assign polar/nonpolar properties to a component based on separation scheme o Utilize qualitative analytical tests- chemical tests, ie. color change, precipitate that indicates a particular component is present  Safety and Waste Disposal o Review safety agreement, especially sections on chemical spills, protective clothing, and injuries o Follow instructions for disposal of various materials o Since a volatile organic solvent will be used (ethyl acetate), no open flames should be used, work in fume hood  Keep boiling beakers from boiling over  Components of Milk o Proteins (casein- precipitates, whey protein) o Fats o Carbohydrates (lactose- type of sugar) o Minerals (calcium, phosphate) o Vitamins (fat soluble- behave like nonpolar molecules, water soluble- behave like polar)  Separation diagram o (1) Add acid and heat (gently); filter o (2) Extract with ethyl acetate  Casein precipitates; behaves like micelle- outside somewhat hydrophilic, inside hydrophobic  When acid added to casein, it gains a proton and reacts less with water; loses its hydrophilic outside and becomes neutral o As more casein molecules get protonated, they clump together o Gets big enough that it can’t stay soluble, precipitates  Components of milk that are insoluble in water move inside casein protein  Fats get precipitated with casein by ethyl acetate  Any fat soluble vitamins end up with fats/ethyl acetate; use qualitative tests to detect their presence o (3) Heat vigorously; filter  ???????? +2 and ????????43 present in solution with lactose, along with water-soluble vitamins  Protein Structure o Casein- globular protein found as a colloid (molecules wrap in ball-like structure) o Whey proteins- water-soluble, smaller in mass o Amino acids make up proteins  Amino acid condensation- forms water − +  Two amino acids react, one loses ???????? , one loses ???? , water forms  Peptide bond forms between remaining parts of amino acids to make protein or part of one  Proteins are long polypeptides o Proteins can have sheet-like (primary) or helix (secondary) shapes, among others  Interaction of primary/secondary structures lead to tertiary or quaternary structures  Often keep reacting and becoming more complex o Fibrous and globular proteins  Lactose o Type of complex carbohydrate o Organic molecule, water-soluble  Almost every part has ????????s and ????s- gives the name carbohydrates o Made up of 2 simpler sugars o Galactose + glucose = lactose  Enzyme lactase used to separate lactose into these  Will use glucose test strip to prove presence of glucose  Qualitative Tests o Fehling’s Test- carbohydrate forms brick-red precipitate  Lactose tested for by testing for glucose after decomposition o Calcium test- treat with ammonium oxalate, white precipitate o Phosphate test- nitric acid and ammonium molybdate, forms precipitate o Biuret Test- ????????????????, ????????????????4give purple-pink color


Buy Material

Are you sure you want to buy this material for

25 Karma

Buy Material

BOOM! Enjoy Your Free Notes!

We've added these Notes to your profile, click here to view them now.


You're already Subscribed!

Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'

Why people love StudySoup

Jim McGreen Ohio University

"Knowing I can count on the Elite Notetaker in my class allows me to focus on what the professor is saying instead of just scribbling notes the whole time and falling behind."

Amaris Trozzo George Washington University

"I made $350 in just two days after posting my first study guide."

Bentley McCaw University of Florida

"I was shooting for a perfect 4.0 GPA this semester. Having StudySoup as a study aid was critical to helping me achieve my goal...and I nailed it!"


"Their 'Elite Notetakers' are making over $1,200/month in sales by creating high quality content that helps their classmates in a time of need."

Become an Elite Notetaker and start selling your notes online!

Refund Policy


All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email


StudySoup has more than 1 million course-specific study resources to help students study smarter. If you’re having trouble finding what you’re looking for, our customer support team can help you find what you need! Feel free to contact them here:

Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to

Satisfaction Guarantee: If you’re not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.

Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.