GEN 3000- CHAPTER 9
GEN 3000- CHAPTER 9 GEN 3000
Popular in Genetics
Popular in Biomedical Sciences
This 2 page Class Notes was uploaded by Jomary Arias on Tuesday February 23, 2016. The Class Notes belongs to GEN 3000 at Clemson University taught by Dr. Kate Tsai in Spring 2016. Since its upload, it has received 48 views. For similar materials see Genetics in Biomedical Sciences at Clemson University.
Reviews for GEN 3000- CHAPTER 9
Report this Material
What is Karma?
Karma is the currency of StudySoup.
You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!
Date Created: 02/23/16
GENETICS 3000 Kate Tsai Chapter 9 Notes CHAPTER 9- Genetic Material Key Terms Tetranucleotide Theory- DNA is made up of units of 4 bases in a fixed sequence DNA vs. Protein as a means of encoding information. DNA- composed of nucleotides that consist of a phosphate, sugar, and nitrogenous- base. Ribose- Sugar + Base, contains Oxygen Deoxyribose- Sugar + Base, does NOT contain Oxygen Phosphate- gives DNA it’s negative charge and makes acidic substance Chargaff’s Rule- A= T, G = C Reassociation Kinetics- the amount of time it takes for hydrogen bonds to renature into a double stranded helix after being denatured using heat. Important People Levene- proposed tetranucleotide theory. Nucleotides are the most basic components of DNA, made of sugar, phosphate and base. Miescher- studied the medical relevance of puss and discovered nuclin (DNA) in the nuclei of WBC. Kossel- determines that DNA contains nitrogenous bases known as Adenine (A), Thymine (T), Guanine (G), and Cytosine (C). There is an equal relationship between bases since they’re fixed and paired. Griffth- demonstrates the transforming principle-early efforts to ID. Used Streptococcus Pneumonia and identified two strains: 1) Virulent Strain- has polysaccharide coat, smooth 2) R Strain- DO Not have polysaccharide coat, rough Some R strains could spontaneously become smooth. Hershey and Chase- demonstrates that DNA is genetic material in viruses Avery MacLeod & McCarty- demonstrated that DNA is the genetic material in bacteria. Isolated transforming principle Chargaff- isolated DNA and found varied in composition. X-ray crystallography was key to unlocking DNA structure, x-rays are beamed at crystal sample and hit detector( photographic plate) resulting in a diffraction pattern. Watson & Crick- used chemistry & X-ray diffraction to solve DNA structure. Solved B- DNA GENETICS 3000 Kate Tsai Chapter 9 Notes Properties of Genetic Material 1. Must be capable of being replicated faithfully 2. Must encode & store complex info. 3. Must contain enough info. To yield phenotype 4. Must have variation as result of mutation (yields various phenotypes because of different alleles Purine Pyrimidine Includes: Adenine (A) and Guanine (G) Includes: Cytosine ( C ), Thymine (T), and Uracil ( U) DNA Structure (Watson & Crick) Pyrimidine + Pyrimidine = DNA too Thin (Small in size) (Small) Purine + Purine = DNA too thick (Large) (Large) Purine + Pyrimidine = DNA thickness compatible with X-ray data DNA polynucleotide strand RNA polynucleotide strand - Contains Sugar- Phosphate backbone - Contains Sugar- Phosphate back bone - Strands run in opposite directions: - Generally single stranded Antiparallel - Thymine is switched for Uracil 3 types of DNA: A Form B Form Z form - Alpha helix - Alpha helix (RIGHT - LEFT handed helix - Shorter & wider spiral) - Zigzag backbone - Probably does NOT - 10 bp per rotation - Sites of active genes exist in nature - Exists in the presence can make Z-DNA of water - Most stable of DNA’s under physiological conditions - Alpha helix -
Are you sure you want to buy this material for
You're already Subscribed!
Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'