Gram Positive Rods Part 1: Listeria, Corynebacterium, Dermatophilus, Nocardia
Gram Positive Rods Part 1: Listeria, Corynebacterium, Dermatophilus, Nocardia CPB 35100
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This 5 page Class Notes was uploaded by Jennie Edmanson on Monday February 9, 2015. The Class Notes belongs to CPB 35100 at Purdue University taught by Dr. Tracy Vemulapalli in Spring2015. Since its upload, it has received 172 views. For similar materials see Microbiology for Veterinary Technicians in Science at Purdue University.
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Date Created: 02/09/15
Gram Positive Rods Part 1 Listeria Corynebacterium Dermatophilus Nocardia l Listeria monocytogenes a totmgr Gram positive rod Nonspore forming Frequently in chains Anaerobic Motile Common pathogen Hosts i Various Most often domestic ruminants ii Humans zoonotic Outcome depends on immune status of host Saprophyte An organism especially a fungus or bacterium that grows on and derives its nourishment from dead or decaying organic matter Environmental contaminant i Air soil water ii Poor quality silage iii Feces of cows 1 Fecal oral route iv Deli meats zoonosis Pathogenesis i Immune competent nonruminant I clearance of pathogen via macrophages ii Immune competent ruminant I results in circling disease iii Immunosuppressed I Meningitis iv Pregnant women I late term abortion Diagnosis i Culturesensitivity ii Gram stain iii Biochemical tests iv Motility 1 Motility tests a Hanging drop method b Stab tube method m Treatment II i Antibiotics Preventive strategies i Avoid poor quality silage ii Vaccine cattle iii Avoid deli meats during pregnancy and when immunocompromised 2 Corynebacterium W Gram positive rod Aerobic Pleomorphic i ii Coccoid Club shaped diptheroidal Non spore forming Cell wall contains corynemycolic acids Corynebacterium renale i ii iii iv Ecology J Considered normal ora of lower urogenital tract 2 Opportunistic diseaseinfection Transmission 1 Ascending infection of normal ora 2 Direct contact and subsequent ascending infection 3 Others 61 Venereal b C renale surVives well in soil indirect transmission Disease 1 Cystitis 2 Ascending pyelonephritis 3 Herd incidence 15 Cattle 1 Places for urine to pool 2 Shortness of female urethra 3 Damage during pregnancyparturition 4 Anatomic abnormalities 5 Urinary tract obstruction Clinical presentation 1 Acute kidney infection pyelonephritis a Fever 9 Anorexia c Polyuria d Hematuriapyuria e Arched back indicating pain 2 Chronic pyelonephritis a Anorexiaweight loss I Decreased milk production 3 Pizzle rot sheath rot 61 Sheep goats intact and castrated 9 Agents 139 C pilosum ii C cystitidis c Opportunistic infection i Preputial ulcerative dermatitis males vi ii Ulcerative vulvovaginitis females breeding d Transmission i Contact Prevention 1 Normalcommensalsl management and decreased stress client education know this g Corynebacterium pseudotuberculosis i ii iii iv vi vii viii Facultative intracellular parasite 1 Requires an obligate host sheep goats Cause of Caseous Lymphadenitis CLA Transmission 1 Direct contact with abscessed materials 2 Fomites a Sheep dip b Sheers c Feeders Pathogenesis 1 Entry skin multiplication local I phagocytized by macrophage I 1 sm CFUs intracellular survival with in phagolysosome I increased local blood vessel permeability I regional lymph nodes I dissemination distant sites 2 Large CFUs APC death I dissemination distant sites Pathology 1 Caseous necrosis a cheeselike appearance b May be surrounded by a fibrous capsule Clinical Disease 1 3050 in affected ocks a Rate may increase with advancing age of herd 2 Chronic systemic I thin ewe syndrome I wasting and death 3 Client Education a Cost of CLA Carcass condemnation slaughter Decreased production wool and milk Leather trade scaring of hide decrease in quality Decreased reproductive performance 9906 Diagnosis 1 Culture and sensitivity 2 Betahemolysin inhibition a CAMP Test in reverse Treatment 1 Isolate all affected animals 2 Lance and ush abscesses a Preferably on concrete oor easy cleanup of pus 3 Antibiotics a Poor penetration in large caseating abscesses 4 Zoonotic ix Prevention 1 Disinfection of potential fomites 2 Cull chronically infected 3 Vaccinate 90 effective 3 Dermatophilus a Aerobic b Gram positive c Branching filamentous rods d Motile zoospores i Zoospores an independently motile spore especially a motile asexual spore esp of algae or lower fungus e Life cycle i Most likely saprophyte in the soil ii Usually associated with we condtions iii Poor pathogen 1 Needs a predisposing factor iv Superficial infections f Dermophilus congolensis i Spread by direct contact and fomites ii Risk factors 1 Infection enhanced by immunosuppression via ticks 2 Wet environment tropical and subtropical areas like Florida 3 Mechanical trauma a Scratches and y bites iii Clinical signs 1 Germination of zoospores in cut I growth through epidermis 2 Characterized by a Matting of hair or wool b Scab and crust formation 3 If in woolcovered areas I lumpy wool disease a Other names Lumpy wool rain rot strawberry foot rot iv Nursing care 1 Remove scabs with mild soap and water 2 Topical application of iodine and copper sulfate v Client education 1 Clinical course can be quite long up to 46 weeks 2 There is no vaccine 3 You don t usually get reinfected 4 Antibiotics only if secondary infection is severe 4 Nocardia a Gram positive Pleomorphic i Rods cocci diptheroids Strict aerobe Nonmotile Saprophyte i Soil decaying vegetation animal feces Nocardia asteroides Dogs i Disease progression and clinical signs 1 Inoculation from soil appearance of a hard nodulepustule I ruptures and pus I multiple modules connect by sinus tracts 2 Occasionally becomes systemic a Lung heart other organs and CNs 3 NOT COMMUNICABLE 4 Systemic a Horses i Skin infection and lymph node abscessation 1 Common presentations ii Occasionally immunosuppressed 1 Respiratory 2 Disseminated disease 5 Diagnosis a Take the correct sample and to take it correctly b FNA c Culture i Blood agar prolonged incubation up to 2 weeks ii Will grow on Sabarouds d Staining Gram and Acidfast 6 Client Education a Long term antibacterial therapy b Some patients Will not respond they require more intensive therapies
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