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Week 10 Lecture Notes for BISC 300

by: Jj Lynch

Week 10 Lecture Notes for BISC 300 BISC300

Marketplace > University of Delaware > Biological Sciences > BISC300 > Week 10 Lecture Notes for BISC 300
Jj Lynch

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About this Document

no friday notes because we only did review that day
Carlton Cooper
Class Notes
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This 2 page Class Notes was uploaded by Jj Lynch on Sunday April 17, 2016. The Class Notes belongs to BISC300 at University of Delaware taught by Carlton Cooper in Summer 2015. Since its upload, it has received 9 views. For similar materials see Microbiology in Biological Sciences at University of Delaware.

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Date Created: 04/17/16
Monday Notes  Restriction enzymes are for bacterial immunity against phages  Conjugation o Cell to cell contact o F+ has pilus, coded for in F factor (plasmid), F plasmid o Plasmid replicates and goes through pilus to reassemble in F- o Recipient cells (F-) have receptors on their membrane but lose them when they get and F factor  F+ only conjugates with F-  When F factor integrates into chromosome it’s called Hfr (high frequency recombination) o Still make pilus o Is able to conjugate genes from chromosome piece pulled with factor  More time in contact=more genes transferred  Drug resistance o Keeps drug out of bacteria o Pump out drug (efflux pump, active transport, multi drug resistance) o Inactivate drug through chemical modification (enzymes) o Modify target enzymes or organelle drug would effect o Change metabolic pathway or increase production of metabolic drug targets  These abilities are coded for on the plasmid  Log phase longer when exposed to drugs because all ATP are going to efflux pump, don’t have enough ATP to grow fast Wednesday Notes  Mechanisms of viral diversity  RNA virus (+ or – single strand) o – has to be converted to + and + is treated like mRNA and goes to ribosome to make proteins  to replicate + or – a double strand intermediate is needed (toxic to eukaryotic cells  antigenic drift- elicits immune response, slow change over time form error in RNA replicase  antigenic shift-sudden and drastic  AIDS o Caused by HIV  RNA retroviridae  HIV-1 evolved from chimp SIVcpz  Group M HIV-1 in US  HIV-2 in Africa (not as pathogenic)  HIV life cycle o Provirus-after it’s integrated into host chromosome o Latent-has no symptoms o Chronic infection- starts symptoms o Causes synthesis of viral RNA-viral proteins and viruses that bud from cell  Flu o In respiratory system  RNA, single stranded, 8 genomes o Life cycle  Subtypes: hemaglutinin (HA) and neuraminidase (NA)  Glycoproteins on surface  Function in attachment and virulence  8 genomes are helical capsids  Antigenic shift, multiple virus genomes infect one host and mix into new strain (?????)  Coldnaked, icosahedral, single stranded + RNA, antigenic drift  Flu  helical, enveloped, segmented – RNA, antigenic drift and shift


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