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by: Matt Pawlush

Translation BIOSCI0716

Marketplace > Biology > BIOSCI0716 > Translation
Matt Pawlush
GPA 101.3
Biology II
Dr. Curto

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About this Document

Biology II
Dr. Curto
Class Notes
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This 2 page Class Notes was uploaded by Matt Pawlush on Friday February 20, 2015. The Class Notes belongs to BIOSCI0716 at a university taught by Dr. Curto in Fall. Since its upload, it has received 187 views.


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Date Created: 02/20/15
Translation Ribosomes are RNAprotein aggregates which function as polypeptide making factories Free ribosomes create proteins for use in the cytoplasm Bound ribosomes create proteins destined for the plasma membrane to be secreted the plasma membrane receptor placement into vesicles or organelles Triplet code in DNA amp RNA Codons and Anticodons Sometimes there are more than one codon for an amino acid Some amino acids only have one codon The correct amino acid is matched with the codon and amino acids are attached together tRNACrick s adapting molecule 7595 bases long with a 5 and 3 end the 3 end always terminates with the sequence CCA 3 hydroxyl of ribose on A attaches to the amino acid There is an acceptor arm and an anticodon arm Wobble hypothesis 5 base on the anticodon does not have to base pair correctly An enzyme unites tRNA with its amino acid Typically there are 20 enzymes one for each amino acid AminoacyltRNA synthetase activates the amino acid It is their substrate specificity that brings the correct amino acid together with its tRNA Ribosomes are complexes of RNA and protein made of two subunits a large and a small The phases of translation are Initiation getting everyone together Elongation polypeptide lengthening Termination finishing up 5 AGGAGGU 3 on the mRNA is a ShineDalgarno sequence in prokaryotes ribosome attaches here for translation small ribosomal subunit binds to the 5 cap in Eukaryotes and looks for the start sequence Elongation A site Arrival of incoming tRNA P site Beptide bond formation gt E site Exit tRNA molecule Peptide bond formation in translation is accomplished by an RNA ribozyme The enzyme is called Peptidyl Transferase and is the catalytic portion on the small subunit A hydrolytic reaction connects amino acids 5 is the amino end 3 is the carboxyl Methionine is usually removed and not present in a mature protein PostTranslation modifications Chemical modifications of certain amino acids by addition of lipids sugars or phosphate groups Removal of one or more amino acids single polypeptide cut into 2 or more pieces signal peptides direct location of protein targeting Many drugs are made to target post translational modifications


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