Scaling BIO 152
Popular in Biology of the organism
Popular in Biology
This 2 page Class Notes was uploaded by Samuel Croteau on Monday May 16, 2016. The Class Notes belongs to BIO 152 at Massachusetts College of Pharmacy & Health Sciences taught by Dr. Demasi in Fall 2016. Since its upload, it has received 11 views. For similar materials see Biology of the organism in Biology at Massachusetts College of Pharmacy & Health Sciences.
Reviews for Scaling
Report this Material
What is Karma?
Karma is the currency of StudySoup.
You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!
Date Created: 05/16/16
Melanie LeMay Scaling Reaction Calculations Purpose: The purpose of scaling a reaction down in size or increasing it is to be able to run an experimental procedure that fits the experimenters needs. If the procedure was run on a large scale previously, but only a small portion of the yield was needed, it can be scaled down to create less waste and use less of the reactants. To Perform Scaling: Simple stoichiometric calculations are required for scaling a reaction, it can be done in two different ways. 1. Calculate and determine the reagent being used, the amount used, molecular weight, and moles to ensure that the literature values are accurate and correct. 2. Calculate the theoretical yield. Moles of product expected (molecular weight of product) = theoretical yield. 3. You can then calculate the needed amounts of reactants by using proportions, the theoretical yield, and known literature values. Ex: If you want 11 grams of NaThy, you set up the proportion accordingly to determine how many grams of NaOH you would need. 11 ? 63 26 g NaOH The first part of the proportion remains the same when trying to find how many grams of Thyminate would be needed in the reaction to yield 11 grams of the NaThy. 11 ? 63 83 grams Thy The bottom number in the proportion to help determine the grams of reactant needed is the molecular weight of the reactant that is trying to be determined. The proportion is also the same when being used to determine how many ml of different solvents are needed as well. In the examples case, 400 ml of H2O was used and 500 ml of EtOH. To scale these down to fit the reaction size, the amount in the literature was placed on the bottom, and X was found. Calculations not using proportions (between different reactions): To calculate not using proportions for calculating between different reactions. To find 11 what is needed to crate 11 grams of NaThy, 11 grams is placed over x which is set equal to the number of moles 0.65. 11 grams = 0.65 moles X= 17 grams X Stoichiometry is then used. To find Thymine needed (17 grams NaThy/ 148 g/mol NaThy ) * (1 mole Thy/1 mole NaThy) * 126 g/mol Thy/ 1 mole) = 14.4 grams of Thymine To find NaOH needed: .11 moles (which was calculated previously) * 40 g/mol NaOH = 4.4 g NaOH needed for the reaction.
Are you sure you want to buy this material for
You're already Subscribed!
Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'